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Articles 1 - 3 of 3

Full-Text Articles in Cell Biology

Optimizing Microfluidic Design For Cell Separation, Joseph Wakim, Marisel De Jesus Vega, Nese Orbey, Carol Barry May 2017

Optimizing Microfluidic Design For Cell Separation, Joseph Wakim, Marisel De Jesus Vega, Nese Orbey, Carol Barry

UMass Center for Clinical and Translational Science Research Retreat

To evaluate the performance of various designs of crossflow filtration microfluidic devices, blood flow was modeled using computational fluid dynamics software (COMSOL Multiphysics). Velocity profiles were generated and used to analyze four critical design parameters: pillar size, pillar shape, gap size, and wall length. These parameters were optimized to yield greatest flow from an unfiltered main channel into two filtered side channels of the device, thereby maximizing filtration capacity.

Devices containing pillars of 10 µm diameter yielded a significantly greater filtration capacity than devices with pillars of 20 µm diameter. Flow patterns from the main channel to the side channels ...


Differentiation And Containment Of Derived Pancreatic Beta Cells, Caden Duffy, Alonzo Cook Ph.D. Feb 2017

Differentiation And Containment Of Derived Pancreatic Beta Cells, Caden Duffy, Alonzo Cook Ph.D.

Biomedical Engineering Western Regional Conference

Currently, the only permanent cure for Type 1 Diabetes is a pancreatic or islet transplant. With the shortage of donors, we are progressing research towards alternative therapies by differentiating induced pluripotent stem cells into derived pancreatic β-cells that can be transplanted and used as treatment for Type 1 Diabetes. We are investigating methods to increase the yield of fully derived, insulin producing pancreatic β-cells and are additionally researching the use of hydrophilized expanded polytetrafluoroethylene for use in cell containment devices. These devices could offer a future alternative for islet transplantation in human patients.


High Performance Amphiphilic Polymer/Hydroxyapatite Composite Tissue Scaffolds, Artem B. Kutikov, Jie Song May 2014

High Performance Amphiphilic Polymer/Hydroxyapatite Composite Tissue Scaffolds, Artem B. Kutikov, Jie Song

UMass Center for Clinical and Translational Science Research Retreat

There is a critical clinical need for alternatives to autograft and allograft bone for over 500,000 bone grafting operations performed each year in the United States. Current synthetic bone grafts suffer from poor handling characteristics, brittle mechanical properties, and inconsistent bioactivity. By blending a biodegradable amphiphilic polymer with hydroxyapatite (HA), the main mineral component in bone, we developed an improved synthetic bone graft. The polymer/HA composites were fabricated in both 2-D and 3-D forms by electrospinning and 3-D printing. These materials exhibited unique handling characteristics such as high tensile elasticity (>200% failure strain) and self-stiffening properties upon hydration ...