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Articles 1 - 11 of 11

Full-Text Articles in Cell Biology

#2 - Comparison Of Ion Transport Expression In Lake Sturgeon Exposed To Differing Water Chemistries, Caitlin Forkin Nov 2019

#2 - Comparison Of Ion Transport Expression In Lake Sturgeon Exposed To Differing Water Chemistries, Caitlin Forkin

Georgia Undergraduate Research Conference (GURC)

This project investigated ion uptake by fish endemic to Georgia, known as Lake Sturgeon (Acipenser fulvescens), which were acquired from a hatchery and naturally found in the Coosa River. For this investigation, identical recirculating tank systems provided separated environments for the two types of water treatments. Equal test groups were placed into the different water chemistries, one into the Coosa River and the other into the regular dechlorinated tap water. All subjects consumed the same type of food: Rangen, which is a commercial chow mix, and blood worm. The Coosa River water differs in ionic composition compared to the hatchery ...


Effects Of Fatty Acids From Coconut And Olive Oil On Expression Of The Hmg1 Gene And Feeding Behavior In Tetrahymena Thermophila, Amelia Konen, Taryn Stewart Apr 2019

Effects Of Fatty Acids From Coconut And Olive Oil On Expression Of The Hmg1 Gene And Feeding Behavior In Tetrahymena Thermophila, Amelia Konen, Taryn Stewart

Carroll College Student Undergraduate Research Festival

HMG-CoA reductase is an enzyme involved in the biosynthetic pathways of sterols in many different organisms. The gene in Tetrahymena thermophila which codes for this protein is HMG1, and has many homologs across many other organisms, from fungi to humans. For this project, we wanted to look at the effect of external fatty acids from coconut and olive oil on the expression of HMG1 in T. thermophila. Previous research has shown that exposure to unsaturated oils result in decreased cholesterol levels. We hypothesized that if T. thermophila are introduced to saturated fats and unsaturated fats, they would exhibit a respectively ...


Targeting Pro-Inflammatory Function Of Microglia Using Small Molecules To Combat Neurodegeneration, Gabrielle C. Williams, Priya Prakash, Gaurav Chopra Aug 2018

Targeting Pro-Inflammatory Function Of Microglia Using Small Molecules To Combat Neurodegeneration, Gabrielle C. Williams, Priya Prakash, Gaurav Chopra

The Summer Undergraduate Research Fellowship (SURF) Symposium

Microglia are the brain’s resident immune cells that are responsible for maintaining homeostasis in healthy conditions. During injury or infection, resting microglia get activated and produce pro-inflammatory cytokines such as IL-1b, IL-1a, IL-6, etc. along with reactive oxygen species like nitric oxide (NO) to combat neuroinflammatory diseases such as Alzheimer’s disease (AD). Inflammation is characterized by the activation of resident-immune cells in the brain called microglia that respond to the eat-me signals released by the toxic amyloid beta peptides as well as the dying neurons in the microenvironment. Recent studies have shown that activated microglia induce neuronal death ...


The Effect Of Alcohol On Pfk1 Gene Expression And Feeding Activity In Tetrahymena Thermophila, Evelyn Sowers, Alex Skoulis Apr 2018

The Effect Of Alcohol On Pfk1 Gene Expression And Feeding Activity In Tetrahymena Thermophila, Evelyn Sowers, Alex Skoulis

Carroll College Student Undergraduate Research Festival

The purpose of this experiment was to test whether alcohol induces starvation conditions in Tetrahymena thermophila. Prior research has shown that exposure to alcohol results in decreases in both the frequency of feeding and overall growth. For this experiment, it was hypothesized that these effects are due to a lack of energy available in the organism for feeding. This hypothesis was tested by monitoring food vacuole formation and expression of the PFK-1 gene in Tetrahymena that were exposed to alcohol. The PFK-1 gene was chosen because its encoded protein plays an essential role in cellular metabolism. For the experiment, control ...


Semaphorin3a Increases Focal Adhesion Formation To Shift The Relationship Between Cell Migration And Substratum Concentration Through A Rock-Dependent Mechanism, Frances V. Compere, Scott Gehler Jun 2016

Semaphorin3a Increases Focal Adhesion Formation To Shift The Relationship Between Cell Migration And Substratum Concentration Through A Rock-Dependent Mechanism, Frances V. Compere, Scott Gehler

Celebration of Learning

Cell migration is essential for many life processes, including wound healing, embryonic development and cancer metastasis. Cells move across a surface by interacting and forming adhesions with the molecules in their environment, specifically the extracellular matrix. Past studies have shown that there is an optimal level of cell-substratum adhesive strength that allows for the most cell migration and spreading (DiMilla et al., 1993; Gaudet et al., 2003). The mechanism by which this works is not well understood, however. Semaphorin 3A (Sema3A) has been shown to increase the expression of integrin receptors, which help mediate the formation of the adhesions between ...


Viewing The Extracellular Matrix: An Imaging Method For Tissue Engineering, Michael Drakopoulos, Sarah Calve Aug 2015

Viewing The Extracellular Matrix: An Imaging Method For Tissue Engineering, Michael Drakopoulos, Sarah Calve

The Summer Undergraduate Research Fellowship (SURF) Symposium

The field of regenerative medicine seeks to create replacement tissues and organs, both to repair deficiencies in biological function and to treat structural damage caused by injury. Scaffoldings mimicking extracellular matrix (ECM), the structure to which cells attach to form tissues, have been developed from synthetic polymers and also been prepared by decellularizing adult tissue. However, the structure of ECM undergoes significant remodeling during natural tissue repair, suggesting that ECM-replacement constructs that mirror developing tissues may promote better regeneration than those modeled on adult tissues. This work investigated the effectiveness of a method of viewing the extracellular matrix of developing ...


Electrophoresis Staining: A New Method Of Whole Mount Staining, Mitchell G. Ayers, Sarah Calve, Zhiyu Li Aug 2014

Electrophoresis Staining: A New Method Of Whole Mount Staining, Mitchell G. Ayers, Sarah Calve, Zhiyu Li

The Summer Undergraduate Research Fellowship (SURF) Symposium

Advances in tissue clearing techniques have allowed almost a ten-fold increase in the viewing depth of confocal microscopy. This allows for intact cellular structures to be rendered in 3D. However, viewing tissues to this depth is often limited to endogenous fluorescence as passive diffusion of antibodies via whole mount staining can take weeks. Our lab is developing a new method involving electrophoresis as a driving force that will promote active antibody binding deep into tissue, reducing the amount of time needed to stain for cellular structures. Due to the inherent charge within antibodies, they are able to be directionally forced ...


Phenotypic Variation In The Model Organism, Danio Rerio, Rachel D. Champaigne, Kim H. Brown May 2014

Phenotypic Variation In The Model Organism, Danio Rerio, Rachel D. Champaigne, Kim H. Brown

Student Research Symposium

Model organisms are used to study evolutionary conserved traits. Zebrafish (Danio rerio) are used a model organism because of their highly fecundity, external fertilization, and robust nature, making them highly adaptable to environmental and genetic variation. In an effort to limit data variation that lies outside of topic interest, phenotypic measures of variation must be performed, understood, and taken into consideration for future studies. A common measurement of phenotypic variation in fish is in the maximum (Ucrit) swimming speeds. Inter and intra-strain variation in zebrafish Ucrit swimming speeds will be observed in a swim tunnel. Baseline values will be recorded ...


Two Modifications, One Protein: The Multiple Roles The Histone Acetyltransferase, Pcaf, Plays In Post Translational Modifications., Ronald Shanderson Apr 2014

Two Modifications, One Protein: The Multiple Roles The Histone Acetyltransferase, Pcaf, Plays In Post Translational Modifications., Ronald Shanderson

Georgia State Undergraduate Research Conference

No abstract provided.


Investigating Mitochondrial Protein Trafficking In Crithidia Fasciculata, Jeremiah Arnold Apr 2014

Investigating Mitochondrial Protein Trafficking In Crithidia Fasciculata, Jeremiah Arnold

Georgia State Undergraduate Research Conference

No abstract provided.


Phosphorylation Regulates Myosin Driven Organelle Movements, Peter Andrew Duden Mar 2013

Phosphorylation Regulates Myosin Driven Organelle Movements, Peter Andrew Duden

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Cytoplasmic streaming in plant cells is the continuous flow of cytoplasm and organelles throughout the cell, with the first observation of cytoplasmic streaming being publicized in 1774. However, the mechanism of cytoplasmic streaming remained unclear until components of the cytoskeleton were researched. Research now supports that the motive force generating cytoplasmic streaming is the interaction of myosin XI motor proteins with organelles while sliding along actin filaments. From this, a key topic of interest is how myosin driven organelle movement is regulated. Our research focuses on whether phosphorylation affects the regulation of myosin XI motor proteins. Specifically, the goal of ...