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Physiology

GSBS Dissertations and Theses

Cell Cycle

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Full-Text Articles in Cell Biology

Mechanisms Regulating Early Mesendodermal Differentiation Of Human Embryonic Stem Cells: A Dissertation, Jennifer J. Vanoudenhove Jun 2016

Mechanisms Regulating Early Mesendodermal Differentiation Of Human Embryonic Stem Cells: A Dissertation, Jennifer J. Vanoudenhove

GSBS Dissertations and Theses

Key regulatory events take place at very early stages of human embryonic stem cell (hESC) differentiation to accommodate their ability to differentiate into different lineages; this work examines two separate regulatory events.

To investigate precise mechanisms that link alterations in the cell cycle and early differentiation, we examined the initial stages of mesendodermal lineage commitment and observed a cell cycle pause that occurred concurrently with an increase in genes that regulate the G2/M transition, including WEE1. Inhibition of WEE1 prevented the G2 pause. Directed differentiation of hESCs revealed that cells paused during commitment to the endo- and mesodermal, but ...


Cell Size Control In The Fission Yeast Schizosaccharomyces Pombe: A Dissertation, Daniel L. Keifenheim Jun 2015

Cell Size Control In The Fission Yeast Schizosaccharomyces Pombe: A Dissertation, Daniel L. Keifenheim

GSBS Dissertations and Theses

The coordination between cell growth and division is a highly regulated process that is intimately linked to the cell cycle. Efforts to identify an independent mechanism that measures cell size have been unsuccessful. Instead, we propose that size control is an intrinsic function of the basic cell cycle machinery.

My work shows that in the fission yeast Schizosaccharomyces pombe Cdc25 accumulates in a size dependent manner. This accumulation of Cdc25 occurs over a large range of cell sizes. Additionally, experiments with short pulses of cycloheximide have shown that Cdc25 is an inherently unstable protein that quickly returns to a size ...