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Physiology

GSBS Dissertations and Theses

Carrier Proteins

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Full-Text Articles in Cell Biology

Pos-1 Regulation Of Endo-Mesoderm Identity In C. Elegans: A Dissertation, Ahmed M. Elewa Apr 2014

Pos-1 Regulation Of Endo-Mesoderm Identity In C. Elegans: A Dissertation, Ahmed M. Elewa

GSBS Dissertations and Theses

How do embryos develop with such poise from a single zygote to multiple cells with different identities, and yet survive? At the four-cell stage of the C. elegans embryo, only the blastomere EMS adopts the endo-mesoderm identity. This fate requires SKN-1, the master regulator of endoderm and mesoderm differentiation. However, in the absence of the RNA binding protein POS-1, EMS fails to fulfill its fate despite the presence of SKN-1. pos-1(-) embryos die gutless. Conversely, the RNA binding protein MEX-5 prevents ectoderm blastomeres from adopting the endo-mesoderm identity by repressing SKN-1. mex-5(-) embryos die with excess muscle at the expense ...


A Role For Intraflagellar Transport Proteins In Mitosis: A Dissertation, Alison R. Bright Jun 2013

A Role For Intraflagellar Transport Proteins In Mitosis: A Dissertation, Alison R. Bright

GSBS Dissertations and Theses

Disruption of cilia proteins results in a range of disorders called ciliopathies. However, the mechanism by which cilia dysfunction contributes to disease is not well understood. Intraflagellar transport (IFT) proteins are required for ciliogenesis. They carry ciliary cargo along the microtubule axoneme while riding microtubule motors. Interestingly, IFT proteins localize to spindle poles in non-ciliated, mitotic cells, suggesting a mitotic function for IFT proteins. Based on their role in cilia, we hypothesized that IFT proteins regulate microtubule-based transport during mitotic spindle assembly. Biochemical investigation revealed that in mitotic cells IFT88, IFT57, IFT52, and IFT20 interact with dynein1, a microtubule motor ...