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Full-Text Articles in Cell Biology

Initiation Of Terpenoid Synthesis In Osmophores Of Stanhopea Anfracta (Orchidaceae): A Cytochemical Study, Kenneth Curry Mar 2012

Initiation Of Terpenoid Synthesis In Osmophores Of Stanhopea Anfracta (Orchidaceae): A Cytochemical Study, Kenneth Curry

Kenneth J. Curry

The terpenoid component of the osmophore fragrance in Stanhopea species is composed of isoprene units synthesized via the mevalonic acid pathway. Localization of hydroxymethylglutaryl CoA synthase in the pathway was achieved using a technique which elicits the formation of an electron- dense precipitate of uranyl ferrocyanide at the point where an acetyl group from acetyl CoA is transferred to acetoacetyl CoA, releasing free CoA-SH. Applying this technique to cells of the osmophore of S. anfracta resulted in a precipitate between the inner and outer mitochondrial membranes, in the smooth endoplasmic reticulum, and, to a lesser extent, on the outer surface ...


Inhibition Of Amyloid-Β Aggregation And Caspase-3 Activation By The Ginkgo Biloba Extract Egb761, Kenneth Curry Mar 2012

Inhibition Of Amyloid-Β Aggregation And Caspase-3 Activation By The Ginkgo Biloba Extract Egb761, Kenneth Curry

Kenneth J. Curry

Standardized extract from the leaves of the Ginkgo biloba tree, labeled EGb761, has been used in clinical trials for its beneficial effects on brain functions, particularly in connection with age-related dementias and Alzheimer's disease (AD). Substantial experimental evidence indicates that EGb761 protects against neuronal damage from a variety of insults, but its cellular and molecular mechanisms remain unknown. Using a neuroblastoma cell line stably expressing an AD-associated double mutation, we report that EGb761 inhibits formation of amyloid-β (Aβ) fibrils, which are the diagnostic, and possibly causative, feature of AD. The decreased Aβ fibrillogenesis in the presence of EGb761 was ...


The Pentameric Vertex Proteins Are Necessary For The Icosahedral Carboxysome Shell To Function As A Co2 Leakage Barrier, Kenneth Curry, Fei Cai, Balaraj Menon, Gordon Cannon, Sabine Heinhorst Mar 2012

The Pentameric Vertex Proteins Are Necessary For The Icosahedral Carboxysome Shell To Function As A Co2 Leakage Barrier, Kenneth Curry, Fei Cai, Balaraj Menon, Gordon Cannon, Sabine Heinhorst

Kenneth J. Curry

Background

Carboxysomes are polyhedral protein microcompartments found in many autotrophic bacteria; they encapsulate the CO2 fixing enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) within a thin protein shell and provide an environment that enhances the catalytic capabilities of the enzyme. Two types of shell protein constituents are common to carboxysomes and related microcompartments of heterotrophic bacteria, and the genes for these proteins are found in a large variety of bacteria.

Methodology/Principal Findings

We have created a Halothiobacillus neapolitanus knockout mutant that does not produce the two paralogous CsoS4 proteins thought to occupy the vertices of the icosahedral carboxysomes and ...