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Helen O'Neill

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Splenic Endothelial Cell Lines Support Development Of Dendritic Cells From Bone Marrow, Geneviève Despars, Helen O'Neill May 2006

Splenic Endothelial Cell Lines Support Development Of Dendritic Cells From Bone Marrow, Geneviève Despars, Helen O'Neill

Helen O'Neill

Although growth factors are commonly used to generate dendritic cells (DCs) in vitro, the role of the microenvironment necessary for DC development is still poorly understood. The mixed splenic stromal cell population STX3 defines an in vitro microenvironment supportive of DC development. Dissection of cellular components of the STX3 stroma should provide information about a niche for DC development. STX3 was therefore cloned by single-cell sorting, and a panel of 102 splenic stromal cell lines was established. Four representative splenic stromal cell lines that support hematopoiesis from bone marrow are described here in terms of stromal cell type and DC ...