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Pharmacology

Alleles; Chaperonins; Endoplasmic Reticulum; Fungal Proteins; Gene Expression Regulation, Fungal; Glycosylation; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins; Heat-Shock Proteins; Membrane Proteins; Mutagenesis; Oligosaccharides; Oxidative Stress; Protein Folding; RNA, Messenger; *Saccharomyces cerevisiae Proteins; Temperature; Yeasts

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Full-Text Articles in Cell Biology

A Role For The Dnaj Homologue Scj1p In Protein Folding In The Yeast Endoplasmic Reticulum, Susana Silberstein, Gabriel Schlenstedt, Pam A. Silver, Reid Gilmore Nov 1998

A Role For The Dnaj Homologue Scj1p In Protein Folding In The Yeast Endoplasmic Reticulum, Susana Silberstein, Gabriel Schlenstedt, Pam A. Silver, Reid Gilmore

Open Access Articles

Members of the eukaryotic heat shock protein 70 family (Hsp70s) are regulated by protein cofactors that contain domains homologous to bacterial DnaJ. Of the three DnaJ homologues in the yeast rough endoplasmic reticulum (RER; Scj1p, Sec63p, and Jem1p), Scj1p is most closely related to DnaJ, hence it is a probable cofactor for Kar2p, the major Hsp70 in the yeast RER. However, the physiological role of Scj1p has remained obscure due to the lack of an obvious defect in Kar2p-mediated pathways in scj1 null mutants. Here, we show that the Deltascj1 mutant is hypersensitive to tunicamycin or mutations that reduce N-linked ...