Open Access. Powered by Scholars. Published by Universities.®

Cell Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 3 of 3

Full-Text Articles in Cell Biology

Modelling Β2ar Regulation, Sharat J. Vayttaden Dec 2011

Modelling Β2ar Regulation, Sharat J. Vayttaden

UT GSBS Dissertations and Theses (Open Access)

The β2 adrenergic receptor (β2AR) regulates smooth muscle relaxation in the vasculature and airways. Long- and Short-acting β-agonists (LABAs/SABAs) are widely used in treatment of chronic obstructive pulmonary disorder (COPD) and asthma. Despite their widespread clinical use we do not understand well the dominant β2AR regulatory pathways that are stimulated during therapy and bring about tachyphylaxis, which is the loss of drug effects. Thus, an understanding of how the β2AR responds to various β-agonists is crucial to their rational use. Towards that end we have developed deterministic models that explore the mechanism of drug- induced β2AR regulation. These mathematical ...


Role Of Rap Signaling In The Regulation Of Erk Activation And Cell-Cell Adhesion, Lisa A. Vuchak May 2011

Role Of Rap Signaling In The Regulation Of Erk Activation And Cell-Cell Adhesion, Lisa A. Vuchak

Publicly Accessible Penn Dissertations

Rap is a member of the Ras family of small GTPases. Mammalian Rap proteins regulate many biological processes including cell and matrix adhesion, migration, proliferation, cytoskeletal dynamics, cell polarity and secretion. Rap1 is activated by TSH and cAMP in thyroid cells independently of PKA. TSH regulates differentiated gene expression and proliferation. Many of these effects are mediated through PKA. There is disagreement as to whether cAMP signaling through Rap contributes to any of the cellular effects of TSH. Given that thyroid cells express high levels of B-Raf and that Rap1 activates ERK through B-Raf in many cells, we investigated whether ...


Compensatory Mechanisms Of Neuroprotection By Pkd Signaling Against Oxidative Damage In Experimental Models Of Parkinson's Disease (Pd): Relevance To Pd Drug Discovery Strategies, Arunkumar Asaithambi Jan 2011

Compensatory Mechanisms Of Neuroprotection By Pkd Signaling Against Oxidative Damage In Experimental Models Of Parkinson's Disease (Pd): Relevance To Pd Drug Discovery Strategies, Arunkumar Asaithambi

Graduate Theses and Dissertations

Oxidative stress is a key pathophysiological mechanism contributing to the selective degeneration of dopaminergic neurons in Parkinson's disease. Unraveling the molecular mechanisms underlying various stages of oxidative neuronal damage is critical to better understanding the diseases and developing new treatment modalities. In this study, we identified that protein kinase D1 (PKD1) functions as a key anti-apoptotic kinase to protect neuronal cells against early stages of oxidative stress. Blockade of PKCδ cleavage, PKCδ knockdown or overexpression of a cleavage-resistant PKCδ mutant effectively attenuated PKD1 activation, indicating that PKCδ proteolytic activation regulates PKD1 phosphorylation. We also identified that phosphorylation of S916 ...