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Full-Text Articles in Cell Biology

Compensatory Mechanisms Of Neuroprotection By Pkd Signaling Against Oxidative Damage In Experimental Models Of Parkinson's Disease (Pd): Relevance To Pd Drug Discovery Strategies, Arunkumar Asaithambi Jan 2011

Compensatory Mechanisms Of Neuroprotection By Pkd Signaling Against Oxidative Damage In Experimental Models Of Parkinson's Disease (Pd): Relevance To Pd Drug Discovery Strategies, Arunkumar Asaithambi

Graduate Theses and Dissertations

Oxidative stress is a key pathophysiological mechanism contributing to the selective degeneration of dopaminergic neurons in Parkinson's disease. Unraveling the molecular mechanisms underlying various stages of oxidative neuronal damage is critical to better understanding the diseases and developing new treatment modalities. In this study, we identified that protein kinase D1 (PKD1) functions as a key anti-apoptotic kinase to protect neuronal cells against early stages of oxidative stress. Blockade of PKCδ cleavage, PKCδ knockdown or overexpression of a cleavage-resistant PKCδ mutant effectively attenuated PKD1 activation, indicating that PKCδ proteolytic activation regulates PKD1 phosphorylation. We also identified that phosphorylation of S916 ...


Echinacea Species And Alkamides Inhibit Prostaglandin E2 Production In Raw264.7 Mouse Macrophage Cells, Carlie A. Lalone, Kimberly D.P. Hammer, Lankun Wu, Jaehood Bae, Norma Leyva, Yi Liu, Avery K.S. Solco, George A. Kraus, Patricia A. Murphy, Eve S. Wurtele, Ok-Kyung Kim, Kwon Ii Seo, Mark P. Widrlechner, Diane F. Birt Jan 2007

Echinacea Species And Alkamides Inhibit Prostaglandin E2 Production In Raw264.7 Mouse Macrophage Cells, Carlie A. Lalone, Kimberly D.P. Hammer, Lankun Wu, Jaehood Bae, Norma Leyva, Yi Liu, Avery K.S. Solco, George A. Kraus, Patricia A. Murphy, Eve S. Wurtele, Ok-Kyung Kim, Kwon Ii Seo, Mark P. Widrlechner, Diane F. Birt

Food Science and Human Nutrition Publications

Inhibition of prostaglandin E2 (PGE2) production in lipopolysaccharide-stimulated RAW264.7 mouse macrophage cells was assessed with an enzyme immunoassay following treatments with Echinacea extracts or synthesized alkamides. Results indicated that ethanol extracts diluted in media to a concentration of 15 μg/mL from E. angustifolia, E. pallida, E. simulata, and E. sanguinea significantly inhibited PGE2 production. In further studies, PGE2 production was significantly reduced by all synthesized alkamides assayed at 50 μM, by Bauer alkamides 8, 12A analogue, and 14, Chen alkamide 2, and Chen alkamide 2 analogue at 25 μM and by Bauer alkamide 14 ...


Mechanisms Of Somatostatin-Induced Paradoxical Increase In Insulin Secretion In The Presence Of Arginine Vasopressin In Clonal [Beta]-Cell Hit-T15 , Henrique Cheng Jan 2002

Mechanisms Of Somatostatin-Induced Paradoxical Increase In Insulin Secretion In The Presence Of Arginine Vasopressin In Clonal [Beta]-Cell Hit-T15 , Henrique Cheng

Retrospective Theses and Dissertations

We investigated the mechanism underlying somatostatin-induced increase in [Ca2+]i leading to insulin release in the presence of arginine vasopressin (AVP) in clonal beta-cell HIT-T15. Somatostatin increased [Ca2+]i and insulin release in a biphasic pattern, characterized by a sharp and transient increase followed by a rapid decline to the sub-basal level. Pretreatment with pertussis toxin, which inactivates Gi/Go, abolished the effects of somatostatin. U-73122, an inhibitor of phospholipase C, antagonized somatostatin-induced increase in [Ca2+] i. In Ca2+-free environment, somatostatin still increased [Ca2+]i, whereas depletion of intracellular Ca 2+ stores with thapsigargin, a microsomal Ca2+ ATPase inhibitor ...


Utilization Of Fatigued And Non-Fatigued Isolated Guinea-Pig Papillary Muscles And Ventricular Myocytes On The Comparison Of Inotropic, Chronotropic And Intracellular Calcium Changes Induced By Monensin And Digoxin , Ismail Meral Jan 1996

Utilization Of Fatigued And Non-Fatigued Isolated Guinea-Pig Papillary Muscles And Ventricular Myocytes On The Comparison Of Inotropic, Chronotropic And Intracellular Calcium Changes Induced By Monensin And Digoxin , Ismail Meral

Retrospective Theses and Dissertations

Experiment 1. We investigated the effects of monensin and digoxin on contraction force (CF), initial contraction velocity (ICV), average contraction velocity (ACV), initial relaxation velocity (IRV) and stimulus to response time (ST) change in fatigued (defined as the level at which papillary muscle contraction had lost 30% of its original contraction force with the elapse of time) and non-fatigued guinea-pig papillary muscles. The five hours of measurement in Experiment 3 were divided into five periods (T0 was equilibration, T1, T2, T3, and T4 were respectively one, two, three, and four hours after drug administration). We found that both monensin and ...