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Cancer Biology Commons

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2007

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Articles 1 - 13 of 13

Full-Text Articles in Cancer Biology

Breast Cancer Cell Proliferation Is Inhibited By Bad: Regulation Of Cyclin D1, R Fernando, Js Foster, A Bible, A Ström, Rg Pestell, M Rao, Arnold Saxton, Seung Baek, K Yamaguchi, Robert Donnell, Maria Cekanova, J Wimalasena Jul 2012

Breast Cancer Cell Proliferation Is Inhibited By Bad: Regulation Of Cyclin D1, R Fernando, Js Foster, A Bible, A Ström, Rg Pestell, M Rao, Arnold Saxton, Seung Baek, K Yamaguchi, Robert Donnell, Maria Cekanova, J Wimalasena

Seung J Baek

Recent investigations suggest that functions of the proapoptotic BCL2 family members, including BAD, are not limited to regulation of apoptosis. Here we demonstrate that BAD inhibits G(1) to S phase transition in MCF7 breast cancer cells independent of apoptosis. BAD overexpression inhibited G(1) transit and cell growth as well as cyclin D1 expression. Inhibition of cyclin D1 expression was mediated through inhibition of transcription activated by AP1. Chromatin immunoprecipitation assays indicated that BAD is localized at the 12-O-tetradecanoylphorbol-13-acetate-response element (TRE) and cAMP-response element (CRE) in the cyclin D1 promoter. This was shown to reflect direct binding interactions of ...


Prostate Cancer Cells Differentally Express Anti-Inflmmatory And Pro-Inflammatory Cytokines And Chemokines: Implications For Prostate Cancer Immunotherapy., Kereen Suzetta Bird-Gordon Dec 2007

Prostate Cancer Cells Differentally Express Anti-Inflmmatory And Pro-Inflammatory Cytokines And Chemokines: Implications For Prostate Cancer Immunotherapy., Kereen Suzetta Bird-Gordon

ETD Collection for AUC Robert W. Woodruff Library

Anti-inflammatory specific cytokines and chemokines are elevated in many advanced tumors and correlate with poor prognosis. However, the differential expression of anti-inflammatory cytokines and chemokines in prostate cancer is not known. We investigated the hypotheses that androgen unresponsive DU145 and PC3 prostate cancer cells and androgen responsive LNCaP prostate cancer cells, differentially expressed selected anti-inflammatory and pro-inflammatory cytokines and chemokines and that, dendritic cells pulsed with prostate tumor antigens will induce mainly pro-inflammatory cytokines and chemokines in T cells using mouse models. Our results indicated that anti-inflammatory specific cytokines IL-1 0, IL-4, and anti-inflammatory specific chemokine CCL- 17 (TARC) and ...


Genistein Affects Her2 Protein Concentration, Activation And Promoter Regulation Via Estrogen Receptor-And Non-Estrogen Receptor-Mediated Mechanisms, Mary S. Sakla, Pete J. Ansell, Nader S. Shenouda, Dennis B. Lubahn, Ruth S. Macdonald Oct 2007

Genistein Affects Her2 Protein Concentration, Activation And Promoter Regulation Via Estrogen Receptor-And Non-Estrogen Receptor-Mediated Mechanisms, Mary S. Sakla, Pete J. Ansell, Nader S. Shenouda, Dennis B. Lubahn, Ruth S. Macdonald

Food Science and Human Nutrition Publications

The HER2 proto-oncogene, a member of the epidermal growth factor receptor family, is overexpressed in 20–30% of breast cancers. Genistein, the main soy isoflavone, interacts with estrogen receptors (ER) and it is also a potent tyrosine kinase inhibitor. Previously, our laboratory found that genistein delayed mammary tumor onset in transgenic mice that overexpress HER2 gene. Our goal was to define the mechanism through which genistein affects mammary tumorigenesis inHER2 overexpressing mice. We hypothesized that genistein inhibits HER2 activation and expression through ER-dependent and ER-independent mechanisms. Genistein inhibited total HER2 protein expression and tyrosine phosphorylation in BT-474, an ERα ...


Breast Cancer Cell Proliferation Is Inhibited By Bad: Regulation Of Cyclin D1, R Fernando, Js Foster, A Bible, A Ström, Rg Pestell, M Rao, Arnold Saxton, Seung Baek, K Yamaguchi, Robert Donnell, Maria Cekanova, J Wimalasena Sep 2007

Breast Cancer Cell Proliferation Is Inhibited By Bad: Regulation Of Cyclin D1, R Fernando, Js Foster, A Bible, A Ström, Rg Pestell, M Rao, Arnold Saxton, Seung Baek, K Yamaguchi, Robert Donnell, Maria Cekanova, J Wimalasena

Maria Cekanova MS, RNDr, PhD

Recent investigations suggest that functions of the proapoptotic BCL2 family members, including BAD, are not limited to regulation of apoptosis. Here we demonstrate that BAD inhibits G(1) to S phase transition in MCF7 breast cancer cells independent of apoptosis. BAD overexpression inhibited G(1) transit and cell growth as well as cyclin D1 expression. Inhibition of cyclin D1 expression was mediated through inhibition of transcription activated by AP1. Chromatin immunoprecipitation assays indicated that BAD is localized at the 12-O-tetradecanoylphorbol-13-acetate-response element (TRE) and cAMP-response element (CRE) in the cyclin D1 promoter. This was shown to reflect direct binding interactions of ...


Identification Of Conserved Gene Expression Features Between Murine Mammary Carcinoma Models And Human Breast Tumors, Jason I. Herschkowitz, Karl Simin, Victor J. Weigman, Igor Mikaelian, Jerry Usary, Zhiyuan Hu, Karen E. Rasmussen, Laundette P. Jones, Shahin Assefnia, Subhashini Chandrasekharan, Michael G. Backlund, Yuzhi Yin, Andrey I. Khramtsov, Roy Bastein, John Quackenbush, Robert I. Glazer, Powel H. Brown, Jeffrey E. Green, Levy Kopelovich, Priscilla A. Furth, Juan P. Palazzo, Olufunmilayo I. Olopade, Philip S. Bernard, Gary A. Churchill, Terry Van Dyke, Charles M. Perou May 2007

Identification Of Conserved Gene Expression Features Between Murine Mammary Carcinoma Models And Human Breast Tumors, Jason I. Herschkowitz, Karl Simin, Victor J. Weigman, Igor Mikaelian, Jerry Usary, Zhiyuan Hu, Karen E. Rasmussen, Laundette P. Jones, Shahin Assefnia, Subhashini Chandrasekharan, Michael G. Backlund, Yuzhi Yin, Andrey I. Khramtsov, Roy Bastein, John Quackenbush, Robert I. Glazer, Powel H. Brown, Jeffrey E. Green, Levy Kopelovich, Priscilla A. Furth, Juan P. Palazzo, Olufunmilayo I. Olopade, Philip S. Bernard, Gary A. Churchill, Terry Van Dyke, Charles M. Perou

Open Access Articles

BACKGROUND: Although numerous mouse models of breast carcinomas have been developed, we do not know the extent to which any faithfully represent clinically significant human phenotypes. To address this need, we characterized mammary tumor gene expression profiles from 13 different murine models using DNA microarrays and compared the resulting data to those from human breast tumors.

RESULTS: Unsupervised hierarchical clustering analysis showed that six models (TgWAP-Myc, TgMMTV-Neu, TgMMTV-PyMT, TgWAP-Int3, TgWAP-Tag, and TgC3(1)-Tag) yielded tumors with distinctive and homogeneous expression patterns within each strain. However, in each of four other models (TgWAP-T121, TgMMTV-Wnt1, Brca1Co/Co;TgMMTV-Cre;p53+/- and DMBA-induced ...


Cooperative Oncogenesis And Polyploidization In Human Cancers: A Dissertation, Susan Ann Heilman May 2007

Cooperative Oncogenesis And Polyploidization In Human Cancers: A Dissertation, Susan Ann Heilman

GSBS Dissertations and Theses

A common phenotype observed in most cancers is chromosomal instability. This includes both structural and numerical chromosomal aberrations, which can promote carcinogenesis. The fusion gene CBFB/MYH11 is created by the structural chromosomal inversion(16)(p13.1q22), resulting in the fusion protein CBFβ-SMMHC, which blocks differentiation in hematopoietic progenitor cells. This mutation alone, however, is not sufficient for transformation, and at least one additional cooperating mutation is necessary.

The role of wildtype Cbfb in modulating the oncogenic function of the fusion protein Cbfβ-SMMHC in mice was examined. Transgenic mice expressing the fusion protein, but lacking a wild-type copy of Cbfb ...


Phytosterol Pygeum Africanum Regulates Prostate Cancer In Vitro And In Vivo, Nader S. Shenouda, Mary S. Sakla, Leslie G. Newton, Cynthia Besch-Williford, Norman M. Greenberg, Ruth S. Macdonald, Dennis B. Lubahn May 2007

Phytosterol Pygeum Africanum Regulates Prostate Cancer In Vitro And In Vivo, Nader S. Shenouda, Mary S. Sakla, Leslie G. Newton, Cynthia Besch-Williford, Norman M. Greenberg, Ruth S. Macdonald, Dennis B. Lubahn

Food Science and Human Nutrition Publications

Background

Prostate cancer is an important public health problem. It is an excellent candidate disease for chemo-prevention because prostate cancer is typically slow growing and is usually diagnosed in elderly males. Pygeum africanum (Prunus africana or Rosaceae) is an African prune (plum) tree found in tropical Africa. An extract from the bark of Pygeum africanum has been used in Europe as a prevention and treatment of prostate disorders including benign prostatic hypertrophy (BPH). More recently in the USA, the phytotherapeutic preparations of Pygeum africanum and Saw palmetto have been marketed for prostate health including prostate cancer prevention and treatment.

Methods ...


Characterization Of Microrna Expression Levels And Their Biological Correlates In Human Cancer Cell Lines., Caifu Chen, Arti Gaur, David Jewell, Yu Liang, Dana Ridzon, Jason Moore, Victor Ambros, Mark Israel Mar 2007

Characterization Of Microrna Expression Levels And Their Biological Correlates In Human Cancer Cell Lines., Caifu Chen, Arti Gaur, David Jewell, Yu Liang, Dana Ridzon, Jason Moore, Victor Ambros, Mark Israel

Victor R. Ambros

MicroRNAs are small noncoding RNAs that function by regulating target gene expression posttranscriptionally. They play a critical role in developmental and physiologic processes and are implicated in the pathogenesis of several human diseases including cancer. We examined the expression profiles of 241 human microRNAs in normal tissues and the NCI-60 panel of human tumor-derived cell lines. To quantify microRNA expression, we employed a highly sensitive technique that uses stem-loop primers for reverse transcription followed by real-time PCR. Most microRNAs were expressed at lower levels in tumor-derived cell lines compared with the corresponding normal tissue. Agglomerative hierarchical clustering analysis of microRNA ...


Overexpressed Raf-1 And Phosphorylated Cyclic Adenosine 3'-5'-Monophosphatate Response Element-Binding Protein Are Early Markers For Lung Adenocarcinoma, Maria Cekanova, M Majidi, T Masi, Hussein Al-Wadei, Hildegard Schuller Mar 2007

Overexpressed Raf-1 And Phosphorylated Cyclic Adenosine 3'-5'-Monophosphatate Response Element-Binding Protein Are Early Markers For Lung Adenocarcinoma, Maria Cekanova, M Majidi, T Masi, Hussein Al-Wadei, Hildegard Schuller

Maria Cekanova MS, RNDr, PhD

BACKGROUND: Pulmonary adenocarcinoma (PAC) is the leading type of lung cancer and has a high mortality. The tobacco carcinogen nicotine-derived nitrosamine 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) stimulates the proliferation of human PAC cells and small airway epithelial cells through beta-1 adrenorecptor-mediated transactivation of the epidermal growth factor receptor (EGFR). METHODS: Using the NNK hamster PAC model and human PAC tissue arrays with matched and unmatched normal lung tissues, the authors tested the hypothesis that Raf-1, an effector of the EGFR, and P-CREB, an effector of the beta-adrenoreceptor, are overexpressed in a significant subset of human PACs and are early ...


The Expression And Function Of Ppar And Hif-1 In Human Melanoma, Caroline Mills Jan 2007

The Expression And Function Of Ppar And Hif-1 In Human Melanoma, Caroline Mills

Theses, Dissertations and Capstones

The first part of my dissertation focuses on the expression and function of PPARs in human melanoma. I found that the A375 cells were significantly growth inhibited in response to PGJ2 and troglitazone treatment. HEMn-LP showed significant growth inhibition in response to troglitazone. I found that PPARγ and PPARδ mRNA is present in both the SK-Mel 28 and A375 cells. The relative level of PPARα mRNA expression is highest in SK-Mel 28 cells, ~3 fold higher relative to both the normal human melanocytes and A375 cells. PPARγ protein was ~50% higher in both SK-Mel 28 and A375 cells relative to ...


Hras Intracellular Trafficking And Signal Transduction, Jodi Ho-Jung Mckay Jan 2007

Hras Intracellular Trafficking And Signal Transduction, Jodi Ho-Jung Mckay

Retrospective Theses and Dissertations

Cancer, which is characterized by uncontrolled cell division and proliferation, is the second leading cause of death in the United States. Of these deaths, approximately 30% are the result of mutations in Ras protein. When activated, Ras propagates multiple signal transduction cascades and induces cell proliferation, differentiation, and apoptosis. Within the Ras subfamily, there are several isoforms of Ras which differ in their posttranslational modifications. The modifications include proteolytic cleavage of the CAAX box, farnesylation, and palmitoylation which act as plasma membrane anchors where most signal transduction takes place. This dissertation demonstrates that Ras endocytosis takes place through a novel ...


Lim Kinase 1 Modulates Expression Of Matrix Metalloproteinases And Associates With Gamma-Tubulin: Dual Role In Invasion And Mito, Tenekua Tapia Jan 2007

Lim Kinase 1 Modulates Expression Of Matrix Metalloproteinases And Associates With Gamma-Tubulin: Dual Role In Invasion And Mito, Tenekua Tapia

Electronic Theses and Dissertations

LIM kinase 1 (LIMK1) is a unique dual specificity serine/threonine kinase containing two N-terminal LIM domains in tandem, a PDZ domain and a C-terminal catalytic domain. LIMK1 is involved in modulation of actin cytoskeleton through inactivating phosphorylation of the ADF (actin depolymerization factor) family protein cofilin. Recent studies have shown that LIMK1 is upregulated in breast and prostate cancer cells and tissues, promotes metastasis in animals and induces acquisition of an invasive phenotype when ectopically expressed in benign prostate epithelial (BPH) cells. Furthermore, overexpression of LIMK1 was associated with altered sub cellular localization of the membrane type 1 matrix ...


Mechanism Of Action And Regulation Of Membrane Serine Protease Prostasin In The Prostate And Prostate Cancer, Mengqian Chen Jan 2007

Mechanism Of Action And Regulation Of Membrane Serine Protease Prostasin In The Prostate And Prostate Cancer, Mengqian Chen

Electronic Theses and Dissertations

The glycosylphosphatidylinositol (GPI)-anchored serine protease prostasin (PRSS8) is expressed at the apical membrane surface of epithelial cells and acts as a suppressor of tumor invasion when re-expressed in highly invasive human prostate and breast cancer cell lines. To better understand the molecular mechanisms underlying the anti-invasion phenotype associated with prostasin re-expression in prostate cancer cells, we expressed wild-type human prostasin or a serine active-site mutant prostasin in the PC-3 human prostate carcinoma cells. Molecular changes were measured at the mRNA and the protein levels. The expression of several invasion-promoting molecules is regulated by prostasin re-expression, mediated by a protein-level ...