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2002

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Articles 1 - 13 of 13

Full-Text Articles in Cancer Biology

Historical Development Of The Linear Nonthreshold Dose-Response Model As Applied To Radiation, Ronald L. Kathren Dec 2002

Historical Development Of The Linear Nonthreshold Dose-Response Model As Applied To Radiation, Ronald L. Kathren

The University of New Hampshire Law Review

[Excerpt] "Despite the nearly universal adoption of the linear nonthreshold dose response model (LNT) as the primary basis for radiation protection standards for the past half century, the LNT remains highly controversial and a contentious topic of discussion among health physicists, radiation biologists, and other radiological scientists. Indeed, it has been pointed out that the LNT has assumed the status of a paradigm, synonymous with an ideal, standard, or paragon or perhaps to some, a sacred cow. Reduced to its very basics, the LNT postulates that every increment of ionizing radiation dose, however small, carries with it a commensurate increase ...


Anisomycin Activates Jnk And Sensitises Du 145 Prostate Carcinoma Cells To Fas Mediated Apoptosis, James Curtin, Thomas Cotter Nov 2002

Anisomycin Activates Jnk And Sensitises Du 145 Prostate Carcinoma Cells To Fas Mediated Apoptosis, James Curtin, Thomas Cotter

Articles

Treatment of the hormone refractory prostate cancer cell line DU 145 with sublethal concentrations of chemotherapeutic drugs has been reported to sensitise these cells to Fas mediated apoptosis. However, the mechanism by which this occurs has not been determined. Our group has shown that inhibition of JNK activity completely abrogates the effects of chemotherapeutic drugs. Using anisomycin, a potent JNK agonist, we have demonstrated a role for JNK in Fas mediated apoptosis in DU 145 cells. Inhibition of Caspase 8 and Caspase 9 completely inhibits this process which suggests that DU 145 cells require mitochondrial amplification of the Fas apoptotic ...


Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder Jul 2002

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder

Maria Cekanova MS, RNDr, PhD

Cyclin E, a G(1) cyclin serving to activate cyclin-dependent kinase 2, is the only cyclin gene for which alternative splicing leading to structurally different proteins has been described. Different cyclin E proteins are present in tumor tissues but absent from normal (steady) tissues. Cyclin E contributes to the regulation of cell proliferation and ongoing differentiation and aging. Because trophoblast has invasive properties and differentiates into syncytium and placental aging may develop at term, we examined cyclin E protein variants in human placenta. Placental samples were collected from 27 deliveries between 33 and 41 wk and were compared with ovarian ...


Integrin (Alpha 6 Beta 4) Regulation Of Eif-4e Activity And Vegf Translation: A Survival Mechanism For Carcinoma Cells, Jun Chung, Robin E. Bachelder, Elizabeth A. Lipscomb, Leslie M. Shaw, Arthur M. Mercurio Jul 2002

Integrin (Alpha 6 Beta 4) Regulation Of Eif-4e Activity And Vegf Translation: A Survival Mechanism For Carcinoma Cells, Jun Chung, Robin E. Bachelder, Elizabeth A. Lipscomb, Leslie M. Shaw, Arthur M. Mercurio

Cancer Biology Publications and Presentations

We define a novel mechanism by which integrins regulate growth factor expression and the survival of carcinoma cells. Specifically, we demonstrate that the alpha 6 beta 4 integrin enhances vascular endothelial growth factor (VEGF) translation in breast carcinoma cells. The mechanism involves the ability of this integrin to stimulate the phosphorylation and inactivation of 4E-binding protein (4E-BP1), a translational repressor that inhibits the function of eukaryotic translation initiation factor 4E (eIF-4E). The regulation of 4E-BP1 phosphorylation by alpha 6 beta 4 derives from the ability of this integrin to activate the PI-3K-Akt pathway and, consequently, the rapamycin-sensitive kinase mTOR that ...


Effect Of Acetazolamide On Hypericin Photocytotoxicity, P Solar, I Cavarga, J Hofmanova, Maria Cekanova, P Miskovsky, P Brezani, G Hrckova, A Kozubik, P Fedorocko Jun 2002

Effect Of Acetazolamide On Hypericin Photocytotoxicity, P Solar, I Cavarga, J Hofmanova, Maria Cekanova, P Miskovsky, P Brezani, G Hrckova, A Kozubik, P Fedorocko

Maria Cekanova MS, RNDr, PhD

The photodynamic action of hypericin (HYP) in vitro was evaluated using human leukemic HL-60 and lung carcinoma A549 cell lines. After illumination HYP (1 x 10 (-5) M) reduced the proliferation and/or survival of HL-60 and A549 cells vs. controls to almost to 0 % and 29 %, respectively. A lower concentration of HYP (1 x 10 (-6) M) decreased the proliferation and/or survival only in HL-60 cells. Non-cytotoxic concentrations of the carbonic anhydrase inhibitor acetazolamide (ACTZ) (1 x 10 (-3)-1 x 10 (-6) M) significantly potentiated these effects of HYP (1 x 10 (-6)M) in HL-60, but ...


Gene Profiling Of Cell Cycle Progression Through S-Phase Reveals Sequential Expression Of Genes Required For Dna Replication And Nucleosome Assembly, Caroline M. J. Van Der Meijden, David S. Lapointe, Mai X. Luong, Daniel Peric-Hupkes, Brian C. Cho, Janet L. Stein, Andre J. Van Wijnen, Gary S. Stein May 2002

Gene Profiling Of Cell Cycle Progression Through S-Phase Reveals Sequential Expression Of Genes Required For Dna Replication And Nucleosome Assembly, Caroline M. J. Van Der Meijden, David S. Lapointe, Mai X. Luong, Daniel Peric-Hupkes, Brian C. Cho, Janet L. Stein, Andre J. Van Wijnen, Gary S. Stein

Open Access Articles

The ordered expression of genes after growth factor stimulation in G(1) supportsthe onset of DNA replication. To characterize regulatory events during S-phase when cell cycle progression has become growth factor independent, we have profiled the expression of over 7,000 human genes using GeneChip DNA microarray analysis. HeLa cells were synchronized at the beginning of S-phase by thymidine/aphidicolin block, and RNA populations were analyzed throughout the S and G(2) phases. Expression of genes involved in DNA replication is maximal during early S-phase, whereas histone mRNAs peak at mid S-phase. Genes related to cell proliferation, including those encoding ...


Forced Expression Of The Interferon Regulatory Factor 2 Oncoprotein Causes Polyploidy And Cell Death In Fdc-P1 Myeloid Hematopoietic Progenitor Cells, Ronglin Xie, Andre J. Van Wijnen, Caroline M. J. Van Der Meijden, Janet L. Stein, Gary S. Stein May 2002

Forced Expression Of The Interferon Regulatory Factor 2 Oncoprotein Causes Polyploidy And Cell Death In Fdc-P1 Myeloid Hematopoietic Progenitor Cells, Ronglin Xie, Andre J. Van Wijnen, Caroline M. J. Van Der Meijden, Janet L. Stein, Gary S. Stein

Open Access Articles

The IFN regulatory factor-2 (IRF-2) oncoprotein controls the cell cycle-dependent expression of histone H4 genes during S phase and may function as a component of an E2F-independent mechanism to regulate cell growth. To investigate the role of IRF-2 in control of cell proliferation, we have constructed a stable FDC-P1 cell line (F2) in which expression of IRF-2 is doxycycline (DOX)-inducible, and a control cell line (F0). Both the F2 and F0 cell lines were synchronized in the G1 phase by isoleucine deprivation, and IRF-2 was induced by DOX on release of cells from the cell cycle block. Flow cytometric ...


Ube1l Is A Retinoid Target That Triggers Pml/Rarα Degradation And Apoptosis In Acute Promyelocytic Leukemia, Sutisak Kitareewan, Ian Pitha-Rowe, David Sekula, Christopher H. Lowrey, Michael J. Nemeth, Todd R. Golub, Sarah J. Freemantle, Ethan Dmitrovsky Mar 2002

Ube1l Is A Retinoid Target That Triggers Pml/Rarα Degradation And Apoptosis In Acute Promyelocytic Leukemia, Sutisak Kitareewan, Ian Pitha-Rowe, David Sekula, Christopher H. Lowrey, Michael J. Nemeth, Todd R. Golub, Sarah J. Freemantle, Ethan Dmitrovsky

Open Dartmouth: Faculty Open Access Scholarship

All-trans-retinoic acid (RA) treatment induces remissions in acute promyelocytic leukemia (APL) cases expressing the t(15;17) product, promyelocytic leukemia (PML)/RA receptor α (RARα). Microarray analyses previously revealed induction of UBE1L (ubiquitin-activating enzyme E1-like) after RA treatment of NB4 APL cells. We report here that this occurs within 3 h in RA-sensitive but not RA-resistant APL cells, implicating UBE1L as a direct retinoid target. A 1.3-kb fragment of the UBE1L promoter was capable of mediating transcriptional response to RA in a retinoid receptor-selective manner. PML/RARα, a repressor of RA target genes, abolished this UBE1L promoter ...


Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder Jan 2002

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

No abstract provided.


Preparation Of Novel Hydroxyethyl Amine Isosteres As Potential Cathepsin D Inhibitors, Rose M. Mcconnell, Walter E. Godwin, Amy Stefan, Crystal Newton, Nikki Herring, Crissy Goss Jan 2002

Preparation Of Novel Hydroxyethyl Amine Isosteres As Potential Cathepsin D Inhibitors, Rose M. Mcconnell, Walter E. Godwin, Amy Stefan, Crystal Newton, Nikki Herring, Crissy Goss

Journal of the Arkansas Academy of Science

Cathepsin D is a lysosomal aspartic protease found in all mammalian cells and is considered to be one of the main catabolic proteinases. Cathepsin D has been suggested to play a role in the metastatic potential of several types of cancer. A high activated cathepsin D level in breast tumor tissue has been associated with an increased incidence of relapse and metastasis. High levels of active cathepsin D have also been found in colon cancer, prostate cancer, uterine cancer, and ovarian cancer. Hydroxyethyl isosteres with cyclic tertiary amine have proven to be clinically useful as inhibitors of aspartyl proteases similar ...


Gold Nanoparticle-Mediated Transfection Of Mammalian Cells, Sallie Smith Schneider, K.K. Sanhu, J.M. Simard, C.M. Mcintosh, V.M. Rotello Dec 2001

Gold Nanoparticle-Mediated Transfection Of Mammalian Cells, Sallie Smith Schneider, K.K. Sanhu, J.M. Simard, C.M. Mcintosh, V.M. Rotello

Sallie W Smith Schneider

No abstract provided.


Regulation Of The Immediate Early Gene Product C-Fos By Erk And Rsk: A Mechanism For Sensing And Propagating Erk Signal Duration, Sallie Smith Schneider, L.O. Murphy, R.H. Chen, D.C. Fingar, J. Blenis Dec 2001

Regulation Of The Immediate Early Gene Product C-Fos By Erk And Rsk: A Mechanism For Sensing And Propagating Erk Signal Duration, Sallie Smith Schneider, L.O. Murphy, R.H. Chen, D.C. Fingar, J. Blenis

Sallie W Smith Schneider

No abstract provided.


Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder Dec 2001

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder

Maria Cekanova MS, RNDr, PhD

No abstract provided.