Open Access. Powered by Scholars. Published by Universities.®

Cancer Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

1999

Discipline
Institution
Keyword
Publication
Publication Type
File Type

Articles 1 - 7 of 7

Full-Text Articles in Cancer Biology

The Ccaat Displacement Protein/Cut Homeodomain Protein Represses Osteocalcin Gene Transcription And Forms Complexes With The Retinoblastoma Protein-Related Protein P107 And Cyclin A, Maria F. Van Gurp, Jitesh Pratap, Mai X. Luong, Amjad Javed, Heidi M. Hoffmann, Antonio Giordano, Janet L. Stein, Ellis J. Neufeld, Jane B. Lian, Gary S. Stein, Andre J. Van Wijnen Dec 1999

The Ccaat Displacement Protein/Cut Homeodomain Protein Represses Osteocalcin Gene Transcription And Forms Complexes With The Retinoblastoma Protein-Related Protein P107 And Cyclin A, Maria F. Van Gurp, Jitesh Pratap, Mai X. Luong, Amjad Javed, Heidi M. Hoffmann, Antonio Giordano, Janet L. Stein, Ellis J. Neufeld, Jane B. Lian, Gary S. Stein, Andre J. Van Wijnen

Open Access Articles

Developmental control of bone tissue-specific genes requires positive and negative regulatory factors to accommodate physiological requirements for the expression or suppression of the encoded proteins. Osteocalcin (OC) gene transcription is restricted to the late stages of osteoblast differentiation. OC gene expression is suppressed in nonosseous cells and osteoprogenitor cells and during the early proliferative stages of bone cell differentiation. The rat OC promoter contains a homeodomain recognition motif within a highly conserved multipartite promoter element (OC box I) that contributes to tissue-specific transcription. In this study, we demonstrate that the CCAAT displacement protein (CDP), a transcription factor related to the ...


The T(8;21) Chromosomal Translocation In Acute Myelogenous Leukemia Modifies Intranuclear Targeting Of The Aml1/Cbfalpha2 Transcription Factor, Sandra Marie Mcneil, Congmei Zeng, Kimberly Stacy Harrington, Scott W. Hiebert, Jane B. Lian, Janet L. Stein, Andre J. Van Wijnen, Gary S. Stein Dec 1999

The T(8;21) Chromosomal Translocation In Acute Myelogenous Leukemia Modifies Intranuclear Targeting Of The Aml1/Cbfalpha2 Transcription Factor, Sandra Marie Mcneil, Congmei Zeng, Kimberly Stacy Harrington, Scott W. Hiebert, Jane B. Lian, Janet L. Stein, Andre J. Van Wijnen, Gary S. Stein

GSBS Student Publications

Targeting of gene regulatory factors to specific intranuclear sites may be critical for the accurate control of gene expression. The acute myelogenous leukemia 8;21 (AML1/ETO) fusion protein is encoded by a rearranged gene created by the ETO chromosomal translocation. This protein lacks the nuclear matrix-targeting signal that directs the AML1 protein to appropriate gene regulatory sites within the nucleus. Here we report that substitution of the chromosome 8-derived ETO protein for the multifunctional C terminus of AML1 precludes targeting of the factor to AML1 subnuclear domains. Instead, the AML1/ETO fusion protein is redirected by the ETO component ...


P53 Inhibits Alpha 6 Beta 4 Integrin Survival Signaling By Promoting The Caspase 3-Dependent Cleavage Of Akt/Pkb, Robin E. Bachelder, Mark J. Ribick, Alessandra Marchetti, Rita Falcioni, Silvia Soddu, Kathryn R. Davis, Arthur M. Mercurio Dec 1999

P53 Inhibits Alpha 6 Beta 4 Integrin Survival Signaling By Promoting The Caspase 3-Dependent Cleavage Of Akt/Pkb, Robin E. Bachelder, Mark J. Ribick, Alessandra Marchetti, Rita Falcioni, Silvia Soddu, Kathryn R. Davis, Arthur M. Mercurio

Cancer Biology Publications and Presentations

Although the interaction of matrix proteins with integrins is known to initiate signaling pathways that are essential for cell survival, a role for tumor suppressors in the regulation of these pathways has not been established. We demonstrate here that p53 can inhibit the survival function of integrins by inducing the caspase-dependent cleavage and inactivation of the serine/threonine kinase AKT/PKB. Specifically, we show that the alpha6beta4 integrin promotes the survival of p53-deficient carcinoma cells by activating AKT/PKB. In contrast, this integrin does not activate AKT/PKB in carcinoma cells that express wild-type p53 and it actually stimulates their ...


Protein Kinase C-Dependent Mobilization Of The Alpha6beta4 Integrin From Hemidesmosomes And Its Association With Actin-Rich Cell Protrusions Drive The Chemotactic Migration Of Carcinoma Cells, Isaac Rabinovitz, Alex Toker, Arthur M. Mercurio Sep 1999

Protein Kinase C-Dependent Mobilization Of The Alpha6beta4 Integrin From Hemidesmosomes And Its Association With Actin-Rich Cell Protrusions Drive The Chemotactic Migration Of Carcinoma Cells, Isaac Rabinovitz, Alex Toker, Arthur M. Mercurio

Cancer Biology Publications and Presentations

We explored the hypothesis that the chemotactic migration of carcinoma cells that assemble hemidesmosomes involves the activation of a signaling pathway that releases the alpha6beta4 integrin from these stable adhesion complexes and promotes its association with F-actin in cell protrusions enabling it to function in migration. Squamous carcinoma-derived A431 cells were used because they express alpha6beta4 and migrate in response to EGF stimulation. Using function-blocking antibodies, we show that the alpha6beta4 integrin participates in EGF-stimulated chemotaxis and is required for lamellae formation on laminin-1. At concentrations of EGF that stimulate A431 chemotaxis ( approximately 1 ng/ml), the alpha6beta4 integrin is ...


Selection For Androgen Receptor Mutations In Prostate Cancers Treated With Androgen Antagonist, Mary-Ellen Taplin, Glenn J. Bubley, Yoo-Joung Ko, Eric J. Small, Melissa P. Upton, Barur R. Rajeshkumar, Steven P. Balk Jun 1999

Selection For Androgen Receptor Mutations In Prostate Cancers Treated With Androgen Antagonist, Mary-Ellen Taplin, Glenn J. Bubley, Yoo-Joung Ko, Eric J. Small, Melissa P. Upton, Barur R. Rajeshkumar, Steven P. Balk

Open Access Articles

The role of androgen receptor (AR) mutations in androgen-independent prostate cancer (PCa) was determined by examining AR transcripts and genes from a large series of bone marrow metastases. Mutations were found in 5 of 16 patients who received combined androgen blockade with the AR antagonist flutamide, and these mutant ARs were strongly stimulated by flutamide. In contrast, the single mutant AR found among 17 patients treated with androgen ablation monotherapy was not flutamide stimulated. Patients with flutamide-stimulated AR mutations responded to subsequent treatment with bicalutamide, an AR antagonist that blocks the mutant ARs. These findings demonstrate that AR mutations occur ...


Evidence That Distinct States Of The Integrin Alpha6beta1 Interact With Laminin And An Adam, M. S. Chen, E. A. Almeida, A. P. Huovila, Y. Takahashi, Leslie M. Shaw, Arthur M. Mercurio, J. M. White Feb 1999

Evidence That Distinct States Of The Integrin Alpha6beta1 Interact With Laminin And An Adam, M. S. Chen, E. A. Almeida, A. P. Huovila, Y. Takahashi, Leslie M. Shaw, Arthur M. Mercurio, J. M. White

Cancer Biology Publications and Presentations

Integrins can exist in different functional states with low or high binding capacity for particular ligands. We previously provided evidence that the integrin alpha6beta1, on mouse eggs and on alpha6-transfected cells, interacted with the disintegrin domain of the sperm surface protein ADAM 2 (fertilin beta). In the present study we tested the hypothesis that different states of alpha6beta1 interact with fertilin and laminin, an extracellular matrix ligand for alpha6beta1. Using alpha6-transfected cells we found that treatments (e.g., with phorbol myristate acetate or MnCl2) that increased adhesion to laminin inhibited sperm binding. Conversely, treatments that inhibited laminin adhesion increased sperm ...


Characterization Of A Mutant T Cell Hybridoma Line With Defects In Tcr-Mediated Apoptotic Pathway, Sallie Smith Schneider, G. Morgan, S.W. Smith, L. Pak, A. Marshak-Rothstein, R. Fissore, B.A. Osborne Dec 1998

Characterization Of A Mutant T Cell Hybridoma Line With Defects In Tcr-Mediated Apoptotic Pathway, Sallie Smith Schneider, G. Morgan, S.W. Smith, L. Pak, A. Marshak-Rothstein, R. Fissore, B.A. Osborne

Sallie W Smith Schneider

No abstract provided.