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Cancer Biology Commons

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1997

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Articles 1 - 8 of 8

Full-Text Articles in Cancer Biology

Cell Cycle Analysis And Synchronization Of Pluripotent Hematopoietic Progenitor Stem Cells, G. Prem Veer Reddy, Cheryl Y. Tiarks, Lizhen Pang, Joanne Wuu, Chung-Cheng Hsieh, Peter J. Quesenberry Oct 1997

Cell Cycle Analysis And Synchronization Of Pluripotent Hematopoietic Progenitor Stem Cells, G. Prem Veer Reddy, Cheryl Y. Tiarks, Lizhen Pang, Joanne Wuu, Chung-Cheng Hsieh, Peter J. Quesenberry

Open Access Articles

Hematopoietic stem cells purified from mouse bone marrow are quiescent with less than 2% of Lin- Hoechst(low)/Rhodamine(low) (Lin- Ho(low)/Rho(low)) and 10% to 15% of Lin-/Sca+ cells in S phase. These cells enter proliferative cycle and progress through G1 and into S phase in the presence of cytokines and 5% heat-inactivated fetal calf serum (HI-FCS). Cytokine-stimulated Lin- Ho(low)/Rho(low) cells took 36 to 40 hours to complete first division and only 12 hours to complete each of 5 subsequent divisions. These cells require 16 to 18 hours to transit through G0/G1 ...


A Framework For Assessing The Rationality Of Judgments In Carcinogenicity Hazard Identification, Douglas J. Crawford-Brown, Kenneth G. Brown Sep 1997

A Framework For Assessing The Rationality Of Judgments In Carcinogenicity Hazard Identification, Douglas J. Crawford-Brown, Kenneth G. Brown

RISK: Health, Safety & Environment (1990-2002)

Arguing that guidelines for identifying carcinogens now lack a philosophically rigorous framework, the authors present an alternative that draws clear attention to the process of reasoning towards judgments of carcinogenicity.


Cytokine-Facilitated Transduction Leads To Low-Level Engraftment In Nonablated Hosts, Ellen L. W. Kittler, Stefan O. Peters, Rowena B. Crittenden, Michelle E. Debatis, Hayley S. Ramshaw, F. Marc Stewart, Peter J. Quesenberry Jul 1997

Cytokine-Facilitated Transduction Leads To Low-Level Engraftment In Nonablated Hosts, Ellen L. W. Kittler, Stefan O. Peters, Rowena B. Crittenden, Michelle E. Debatis, Hayley S. Ramshaw, F. Marc Stewart, Peter J. Quesenberry

Open Access Articles

Using a murine bone marrow transplantation model, we evaluated the long-term engraftment of retrovirally transduced bone marrow cells in nonmyeloablated hosts. Male bone marrow was stimulated in a cocktail of interleukin-3 (IL-3), IL-6, IL-11, and stem cell factor (SCF) for 48 hours, then cocultured on the retroviral producer line MDR18.1 for an additional 24 hours. Functional transduction of hematopoietic progenitors was detected in vitro by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of multiple drug resistance 1 (MDR1) mRNA from high proliferative potential-colony forming cell (HPP-CFC) colonies. After retroviral transduction, male bone marrow cells were injected into nonablated female mice ...


Oral Cancer Screening, Damon Omar Watson Jun 1997

Oral Cancer Screening, Damon Omar Watson

Honors Theses

Cancers of the oral cavity and pharynx have increased throughout the decades. Since oral cancer represents a small percentage of the total cancers in the United States, it is often overlooked. Though this is the case, the results of this cancer can be devastating. To address this issue, an Oral Cancer Screening was organized on August 14, 1996 at the West Michigan Cancer Center in Kalamazoo. This was a joint collaboration between the author, the West Michigan Cancer Center, and the Kalamazoo Valley Dental Society. This event allowed research to take place concerning the data obtained. Confidential results and information ...


Anthropogenic Electromagnetic Fields And Cancer: A Perspective, Charles Tomljanovic, Maxine Wright-Walters, Jules Stephensky Jun 1997

Anthropogenic Electromagnetic Fields And Cancer: A Perspective, Charles Tomljanovic, Maxine Wright-Walters, Jules Stephensky

RISK: Health, Safety & Environment (1990-2002)

The authors review findings of a recent National Research Council report and conclude that, e.g., until a cancer promotion model can be identified for effective testing, the EMF issue will remain open to debate.


Potential And Distribution Of Transplanted Hematopoietic Stem Cells In A Nonablated Mouse Model, Susan K. Nilsson, Mark S. Dooner, Candi Y. Tiarks, Heinz-Ulrich Weier, Peter J. Quesenberry Jun 1997

Potential And Distribution Of Transplanted Hematopoietic Stem Cells In A Nonablated Mouse Model, Susan K. Nilsson, Mark S. Dooner, Candi Y. Tiarks, Heinz-Ulrich Weier, Peter J. Quesenberry

Open Access Articles

Increasingly, allogeneic and even more often autologous bone marrow transplants are being done to correct a wide variety of diseases. In addition, autologous marrow transplants potentially provide an opportune means of delivering genes in transfected, engrafting stem cells. However, despite its widespread clinical use and promising gene therapy applications, relatively little is known about the mechanisms of engraftment in marrow transplant recipients. This is especially so in the nonablated recipient setting. Our data show that purified lineage negative rhodamine 123/Hoechst 33342 dull transplanted hematopoietic stem cells engraft into the marrow of nonablated syngeneic recipients. These cells have multilineage potential ...


Expression Of The Id Family Helix-Loop-Helix Regulators During Growth And Development In The Hematopoietic System, Cathleen L. Cooper, Gerard Brady, Fillio Bilia, Norman N. Iscove, Peter J. Quesenberry May 1997

Expression Of The Id Family Helix-Loop-Helix Regulators During Growth And Development In The Hematopoietic System, Cathleen L. Cooper, Gerard Brady, Fillio Bilia, Norman N. Iscove, Peter J. Quesenberry

Open Access Articles

To better understand the molecular mechanism(s) by which growth and differentiation of the primitive hematopoietic stem cell is initiated, as well as the means by which the maturing cell can commit to development along a specific cell lineage, we elected to study the Id family of helix-loop-helix (HLH) transcriptional regulators. Some members of the HLH family are expressed in a stage-specific manner during hematopoietic development and can regulate the ability of immature hematopoietic cells to terminally differentiate. None of the four Id family genes were detected in the most primitive progenitors. Id-1 was widely expressed in proliferating bi- and ...


Private Pathways To A Common Death, Sallie Smith Schneider, B.A. Osborne Dec 1996

Private Pathways To A Common Death, Sallie Smith Schneider, B.A. Osborne

Sallie W Smith Schneider

No abstract provided.