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Cancer Biology Commons

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Full-Text Articles in Cancer Biology

Post-Transcriptional Regulation Of Glutathione Peroxidase Gene Expression By Selenium In The Hl-60 Human Myeloid Cell Line, Sunil Chada, Constance Whitney, Peter E. Newburger Nov 1989

Post-Transcriptional Regulation Of Glutathione Peroxidase Gene Expression By Selenium In The Hl-60 Human Myeloid Cell Line, Sunil Chada, Constance Whitney, Peter E. Newburger

Open Access Articles

We have used a cloned cDNA for the major human selenoprotein, glutathione peroxidase (GPx), to assess the mode of regulation of human GPx gene (GPX-1) expression by selenium. When the HL-60 human myeloid cell line is grown in a selenium-deficient medium, GPx enzymatic activity decreases 30-fold compared with selenium-replete cells. Upon return to a medium containing selenium in the form of selenite, GPx activity in the cells starts to increase within 48 hours and reaches maximal (selenium-replete) levels at 7 days. Steady-state immunoreactive protein levels correlate with enzymatic activity. Cycloheximide inhibits the rise in GPx activity that accompanies selenium replenishment ...


Reversal Of Multidrug Resistance Using Cyclosporin A, Sara Manley Apr 1989

Reversal Of Multidrug Resistance Using Cyclosporin A, Sara Manley

Life and Environmental Sciences Undergraduate Theses

Multidrug resistance is a phenomenon which occurs in cancer cells. These cells become resistant to the drug of treatment as well as resistant to other unrelated chemotherapeutic drugs. Previous studies have shown that calmodulin inhibitors and calcium channel blockers are able to reverse resistance in these cells when used in conjunction with the chemotherapeutic drug. In this study, the ability of the immunosuppressive drug, cyclosporin A to overcome cellular resistance to the chemotherapeutic drug vincristine was analyzed. The Chinese hamster cell line, DC-3F and its multidrug resistant variant, DC-3F/VCRd-5L were used. Analysis revealed that cyclosporin A caused complete reversal ...


Interferon Gamma And Lipopolysaccharide Promote Macrophage Adherence To Basement Membrane Glycoproteins, Leslie M. Shaw, Arthur M. Mercurio Jan 1989

Interferon Gamma And Lipopolysaccharide Promote Macrophage Adherence To Basement Membrane Glycoproteins, Leslie M. Shaw, Arthur M. Mercurio

Cancer Biology Publications and Presentations

The ability of thioglycollate (TG)-elicited peritoneal macrophages, a population of recently recruited monocytes, to adhere to the basement membrane glycoproteins laminin and type IV collagen is not a constitutive function of these cells. Adherence can be induced, however, by treatment with IFN-gamma and LPS. In general, IFN-gamma is more potent than LPS in promoting this adherence. Maximal adherence, however, is observed when IFN-gamma (greater than or equal to 5 U/ml) is used together with LPS (2.0 ng/ml). These requirements parallel the conditions needed to obtain tumoricidal activation of TG-elicited macrophages. Adherence to laminin, in the presence ...