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Cancer Biology Commons

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Biology

Messiah College

Publication Year

Articles 1 - 3 of 3

Full-Text Articles in Cancer Biology

Prevalence Of A Variant Gastrin Receptor Rna And Correlating Genomic Polymorphism In Human Pancreatic Cancer, Rebekah Jones May 2019

Prevalence Of A Variant Gastrin Receptor Rna And Correlating Genomic Polymorphism In Human Pancreatic Cancer, Rebekah Jones

Honors Projects and Presentations: Undergraduate

Currently, the five-year survival rate of pancreatic cancer is an abysmal 5%. Our lab has focused on the etiology of CCK2i4svR, a hyperactive splice variant of the gastrin receptor (CCK2R), which has been associated with increased pancreatic tumor aggressiveness. To determine if a correlation exists between a single nucleotide polymorphism (SNP) in the receptor, and expression of the variant RNA in patients, we aim to genotype human pancreatic tumor samples and quantify CCK2R and CCK2i4svR mRNA. Initial analysis of RNA samples by Real Time RT‐PCR has demonstrated successful quantification of CCK2R expression in both tumor and normal tissue samples ...


Transfection Of Primary Kidney Cells With Mutated Sv40 T Ag Dna Containing A Cckcr Intron-Derived Sequence, Kaitlyn Grando, Courtnry Burkett, Lawrence Mylin Jan 2015

Transfection Of Primary Kidney Cells With Mutated Sv40 T Ag Dna Containing A Cckcr Intron-Derived Sequence, Kaitlyn Grando, Courtnry Burkett, Lawrence Mylin

Honors Projects and Presentations: Undergraduate

Cholecystokinin-C receptor (CCKCR) is an alternatively spliced version of cholecystokinin-B receptor (CCKBR) found in pancreatic cells. CCKCR is uniquely expressed by cancer cells, making it an important target for immune therapy. Simian Virus 40 Large Tumor Antigen (SV40 T ag) has proven to be a powerful model in the study of cell-mediated immune responses to tumors. Our goal was to construct cells which express a derivative of SV40 T ag that incorporates the retained intron sequence from CCKCR to use as a tool in the study of cellmediated immunity targeting this unique sequence. Two variations of plasmids containing mutated T ...


Construction Of A Novel System To Analyze Recognition Of Mhc Class Ii-Restricted Epitopes By Cd4+ T Cell Hybridoma Clones In Vitro, Samuel Bray, Lawrence Mylin Jan 2013

Construction Of A Novel System To Analyze Recognition Of Mhc Class Ii-Restricted Epitopes By Cd4+ T Cell Hybridoma Clones In Vitro, Samuel Bray, Lawrence Mylin

Honors Projects and Presentations: Undergraduate

The Simian Virus 40 large tumor antigen (SV40 Tag) is an excellent model system for studies in cancer immunology because it is strongly immunogenic, but capable of causing tumors when expressed as a transgene in vivo when protected by immune tolerance. The CD8+ T cell response to Tag in the C57BL/6 murine system is directed against four well-characterized epitopes. However, the CD4+ T cell response in this system remains uncharacterized. Recent work using Tag-specific CD4+ hybridoma clones and immune splenocytes to screen a 175 member, overlapping 15-mer peptide library corresponding to the 708 amino acid Tag protein sequence have ...