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Cancer Biology Commons

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Articles 1 - 5 of 5

Full-Text Articles in Cancer Biology

Impact Of Ros Presence On Oncogenic Ras Activity, Chris Andersen Jan 2018

Impact Of Ros Presence On Oncogenic Ras Activity, Chris Andersen

Summer Research

Previous research has suggested a connection between oncogenic Ras and the cell’s levels of Reactive Oxygen Species (ROS). The underlying cellular mechanism is not well understood. To investigate this connection, we applied the UAS-GAL4 system in Drosophila melanogaster flies to control the expression of Ras and Keap1, a key redox regulator.2 We expected the activity of Ras to vary with its redox environment and thus impact protein activity downstream of Ras signaling cascades. In monitoring three proteins downstream of Ras—Dcp-1, Akt, and MAPK—we aimed to determine which pathways were impacted by ROS modulation.


Using Crispr To Induce A Knock-Out Of Dprl-1 In Drosophila Melanogaster, Alicia Walker Jan 2018

Using Crispr To Induce A Knock-Out Of Dprl-1 In Drosophila Melanogaster, Alicia Walker

Summer Research

Phosphatase of regenerating liver (PRL) is a protein that controls cell processes such as growth and division which has unknown targets. PRL has been found to have both oncogenic and tumor suppressive properties. This study aimed to create a knock out of PRL in Drosohpila melanogaster in order to assess its role in development and in order to illuminate its activity when it is expressed in cancers. We hypothesize that dPRL-1 plays an important role in embryogenesis and that the progeny which lack this gene will be unviable. The CRISPR/Cas9 system was selected as the method in which to ...


5Α-Dihydrotestosterone And The Wnt/Β-Catenin Pathway In Danio Rerio Ovarian Tissue Following 2 And 4 Hour Incubations, Macaulie Casey Jan 2014

5Α-Dihydrotestosterone And The Wnt/Β-Catenin Pathway In Danio Rerio Ovarian Tissue Following 2 And 4 Hour Incubations, Macaulie Casey

Summer Research

The Wnt/β-catenin signaling pathway regulates genes involved in proliferation and apoptosis. These two traits are hallmarks of cancer cells, which grow excessively and demonstrate low mortality. The Wnt/β-catenin pathway has been implicated in ovarian cancer, specifically epithelial ovarian cancer, wherein higher levels of β-catenin may be involved in the development of tumors. Ovarian epithelial tissue also displays high quantities of androgen receptors and thus may be more susceptible to changes in androgen concentration. This study investigated whether exogenous testosterone (DHT) utilized the Wnt/β-catenin pathway in Danio rerio ovarian tissue by monitoring β-catenin and GSK-3β mRNA expression through ...


Examining The Functional Role Of Dprl-1 In Drosophila Melanogaster, John Valenzuela Jan 2013

Examining The Functional Role Of Dprl-1 In Drosophila Melanogaster, John Valenzuela

Summer Research

The Phosphatase of Regenerating Liver (PRL) family of proteins control cell growth, motility and proliferation. They have been shown to elevate the levels of these functions, leading to an increase in cancer metastasis (“malignancy”), when they are overexpressed. The goal of this experiment is to knockout PRL gene expression to examine the general function of PRL proteins. Drosophila melanogaster have only one copy of the PRL gene (dPRL-1), as opposed to humans and other mammals, which have 3. Thus, using P-element imprecise excision to create mutant strains either fully lacking or with decreased function of the dPRL-1 protein, I propose ...


Using Fruit Flies To Investigate A Cancer Metastasis Biomarker: A Study Of Mammalian Prl-3 Function, Chris Large Jan 2013

Using Fruit Flies To Investigate A Cancer Metastasis Biomarker: A Study Of Mammalian Prl-3 Function, Chris Large

Summer Research

Phosphatase of Regenerating Liver 3 (PRL-3), a human protein in the Protein Tyrosine Phosphatase (PTP) gene family, has been highly correlated with cancer’s ability to metastasis in numerous types of cancer. Until recently, this was thought to be the primary function of PRL-3 within mammalian cancer cells, but Basak et al., (2008) found that PRL-3 could have function in inhibiting cellular growth, a contradicting cellular response. Further research was done by Pagarigan et al., (2013) using fruit flies (Drosophila melanogaster) as a model. Their findings echoed these claims and showed that specific structural portions of dPRL-1, fruit flies’ only ...