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Biochemistry, Biophysics, and Structural Biology Commons

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Computational Protein Design To Re-Engineer Stromal Cell-Derived Factor-1Α (Sdf) Generates An Effective And Translatable Angiogenic Polypeptide Analog, William Hiesinger, Jose Manuel Perez Aguilar, Pavan Atluri, Nicole A. Marotta, John R. Frederick, J Raymond Fitzpatrick, Ryan C. Mccormick, Jeffrey R. Muenzer, Elaine C. Yang, Rebecca D. Levit, Li-Jun Yuan, John W. Macarthur, Jeffery G. Saven, Y Joseph Woo Sep 2011

Computational Protein Design To Re-Engineer Stromal Cell-Derived Factor-1Α (Sdf) Generates An Effective And Translatable Angiogenic Polypeptide Analog, William Hiesinger, Jose Manuel Perez Aguilar, Pavan Atluri, Nicole A. Marotta, John R. Frederick, J Raymond Fitzpatrick, Ryan C. Mccormick, Jeffrey R. Muenzer, Elaine C. Yang, Rebecca D. Levit, Li-Jun Yuan, John W. Macarthur, Jeffery G. Saven, Y Joseph Woo

Departmental Papers (Chemistry)

BACKGROUND: Experimentally, exogenous administration of recombinant stromal cell-derived factor-1α (SDF) enhances neovasculogenesis and cardiac function after myocardial infarction. Smaller analogs of SDF may provide translational advantages including enhanced stability and function, ease of synthesis, lower cost, and potential modulated delivery via engineered biomaterials. In this study, computational protein design was used to create a more efficient evolution of the native SDF protein.

METHODS AND RESULTS: Protein structure modeling was used to engineer an SDF polypeptide analog (engineered SDF analog [ESA]) that splices the N-terminus (activation and binding) and C-terminus (extracellular stabilization) with a diproline segment designed to limit the conformational ...


Computational Protein Design: Engineering Molecular Diversity, Nonnatural Enzymes, Nonbiological Cofactor Complexes, And Membrane Proteins, Jeffery G. Saven Jun 2011

Computational Protein Design: Engineering Molecular Diversity, Nonnatural Enzymes, Nonbiological Cofactor Complexes, And Membrane Proteins, Jeffery G. Saven

Departmental Papers (Chemistry)

Computational and theoretical methods are advancing protein design as a means to create and investigate proteins. Such efforts further our capacity to control, design and understand biomolecular structure, sequence and function. Herein, the focus is on some recent applications that involve using theoretical and computational methods to guide the design of protein sequence ensembles, new enzymes, proteins with novel cofactors, and membrane proteins.