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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder Jul 2002

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder

Maria Cekanova MS, RNDr, PhD

Cyclin E, a G(1) cyclin serving to activate cyclin-dependent kinase 2, is the only cyclin gene for which alternative splicing leading to structurally different proteins has been described. Different cyclin E proteins are present in tumor tissues but absent from normal (steady) tissues. Cyclin E contributes to the regulation of cell proliferation and ongoing differentiation and aging. Because trophoblast has invasive properties and differentiates into syncytium and placental aging may develop at term, we examined cyclin E protein variants in human placenta. Placental samples were collected from 27 deliveries between 33 and 41 wk and were compared with ovarian ...


Effect Of Acetazolamide On Hypericin Photocytotoxicity, P Solar, I Cavarga, J Hofmanova, Maria Cekanova, P Miskovsky, P Brezani, G Hrckova, A Kozubik, P Fedorocko Jun 2002

Effect Of Acetazolamide On Hypericin Photocytotoxicity, P Solar, I Cavarga, J Hofmanova, Maria Cekanova, P Miskovsky, P Brezani, G Hrckova, A Kozubik, P Fedorocko

Maria Cekanova MS, RNDr, PhD

The photodynamic action of hypericin (HYP) in vitro was evaluated using human leukemic HL-60 and lung carcinoma A549 cell lines. After illumination HYP (1 x 10 (-5) M) reduced the proliferation and/or survival of HL-60 and A549 cells vs. controls to almost to 0 % and 29 %, respectively. A lower concentration of HYP (1 x 10 (-6) M) decreased the proliferation and/or survival only in HL-60 cells. Non-cytotoxic concentrations of the carbonic anhydrase inhibitor acetazolamide (ACTZ) (1 x 10 (-3)-1 x 10 (-6) M) significantly potentiated these effects of HYP (1 x 10 (-6)M) in HL-60, but ...


Composition And Method For The Prevention And Treatment Of Oxidative Damage In Ocular Tissues, Marjorie F. Lou, Nalini Raghavachari;, Fengyu Qiao Apr 2002

Composition And Method For The Prevention And Treatment Of Oxidative Damage In Ocular Tissues, Marjorie F. Lou, Nalini Raghavachari;, Fengyu Qiao

Papers in Veterinary and Biomedical Science

Thioltransferase and derivatives thereof are provided. Methods of treating or preventing cataract formation comprosing administering thioltransferase or a derivative thereof are also provided. Thioltransferase or derivatives thereof are also useful for treating or preventing diseases resulting from or associated with oxidative stress. Human lens thioltransferase and a DNA sequence encoding the same are also provided.


Osmosensitive Release Of Neurotransmitter Amino Acids: Relevance And Mechanisms, Herminia Pasantes-Morales, Rodrigo Franco, Lenin Ochoa, Benito Ordaz Feb 2002

Osmosensitive Release Of Neurotransmitter Amino Acids: Relevance And Mechanisms, Herminia Pasantes-Morales, Rodrigo Franco, Lenin Ochoa, Benito Ordaz

Papers in Veterinary and Biomedical Science

Hyposmolarity activates amino acid efflux as part of the corrective volume process in a variety of cells. This review discusses the mechanism of amino acid release in brain cells preparations. Results present evidence of substantial differences between the efflux of taurine and that of GABA and glu-tamate, which besides a possible role as osmolytes, have a main function as synaptic transmitters. The differences found concern the efflux time course, the sensitivity to Cl channel blockers, the modulation by tyrosine kinases, the influence of PKC and the effect of cytoskeleton disruptive agents. While taurine efflux features fit well with the mechanisms ...


Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder Jan 2002

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

No abstract provided.


A Candidate Gene Analysis For Response To Salmonella Enteritidis Challenge Or Vaccination In Young Chicks , Wei Liu Jan 2002

A Candidate Gene Analysis For Response To Salmonella Enteritidis Challenge Or Vaccination In Young Chicks , Wei Liu

Retrospective Theses and Dissertations

Salmonella enteritidis (SE) contamination of poultry products is a major cause of food-borne disease worldwide. The candidate gene approach for identification of quantitative trait loci (QTL) for chicken response to Salmonella enteritidis (SE) was applied in the current study. The Iowa Salmonella Response Resource Population (ISRRP), formed by crossing outbred broiler sires with three diverse, highly inbred dam lines (two major histocompatibility complex-congenic Leghorn lines G-B1 and G-B2 and one Fayoumi line) was studied. The F1 generation was evaluated as young chicks for either bacterial load in spleen and cecum after pathogenic SE inoculation, or antibody level after SE vaccination ...


Mechanisms Counteracting Swelling In Brain Cells During Hyponatremia, Herminia Pasantes-Morales, Rodrigo Franco, Benito Ordaz, Lenin D. Ochoa Jan 2002

Mechanisms Counteracting Swelling In Brain Cells During Hyponatremia, Herminia Pasantes-Morales, Rodrigo Franco, Benito Ordaz, Lenin D. Ochoa

Papers in Veterinary and Biomedical Science

Water gain in the brain consequent to hyponatremia is counteracted by mechanisms that initially include a compensatory displacement of liquid from the interstitial space to cerebrospinal fluid and systemic circulation and subsequently an active reduction in cell water accomplished by extrusion of intracellular osmolytes to reach osmotic equilibrium. Potassium (K+), chloride (Cl), amino acids, polyalcohols, and methylamines all contribute to volume regulation, with a major contribution of ions at the early phase and of organic osmolytes at the late phase of the regulatory process. Experimental models in vitro show that osmolyte fluxes occur via leak pathways for organic osmolytes and ...


Influence Of Protein Tyrosine Kinases On Cell Volume Change-Induced Taurine Release, Herminia Pasantes-Morales, Rodrigo Franco Jan 2002

Influence Of Protein Tyrosine Kinases On Cell Volume Change-Induced Taurine Release, Herminia Pasantes-Morales, Rodrigo Franco

Papers in Veterinary and Biomedical Science

Taurine efflux occurs in association with cell swelling in both hyposmotic and isosmotic conditions and during cell shrinkage in apoptotic death. Release occurs through a leak pathway, is largely Ca2+-independent and is sensitive to Cl channel blockers. Taurine efflux elicited by hyposmolarity is reduced or suppressed by tyrosine kinase blockers and increased by tyrosine phosphatase inhibitors. The specific kinases involved are still unknown and may be different in the various cell types. Non-receptor and scr-related protein kinases have been identified in some cells as elements that directly phosphorylate the taurine efflux pathway. Possible tyrosine kinase targets are the ...


Repeated Intravenous Doses Of All-Trans-Retinoyl Β-D-Glucuronide Is Not Effective In The Treatment Of Bacterial Bronchopneumonia In Lambs But Is Devoid Of Gross And Acute Toxicity, Jack M. Gallup, Branka Grubor, Arun B. Barua, Gholamreza Mohammadi, Kim A. Brogden, James A. Olson, Mark R. Ackermann Jan 2002

Repeated Intravenous Doses Of All-Trans-Retinoyl Β-D-Glucuronide Is Not Effective In The Treatment Of Bacterial Bronchopneumonia In Lambs But Is Devoid Of Gross And Acute Toxicity, Jack M. Gallup, Branka Grubor, Arun B. Barua, Gholamreza Mohammadi, Kim A. Brogden, James A. Olson, Mark R. Ackermann

Veterinary Pathology Publications and Papers

Background
All-trans-retinoyl β-D-glucuronide, a water-soluble glucuronic acid conjugate of all-transretinoic acid, has demonstrated high biological activity and low toxicity in most in vitro and in vivo models. Since the reparative effects of retinoids on epithelium are well-known, our aim was to study the effect(s) of intravenously-administered all-trans-retinoyl β-D-glucuronide in lambs with chronic bacterial bronchopneumonia.

Material/Methods
Two groups of lambs were inoculated intrabronchially with either pyrogen-free saline or Mannheimia haemolytica. Thirty-three days later, lambs were injected four times at five-day intervals with 2 mL of 116 mM all-trans-retinoyl β-D-glucuronide (6.0–9.3 µmol/kg or 2.86–4 ...


Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder Dec 2001

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder

Maria Cekanova MS, RNDr, PhD

No abstract provided.