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1994

GSBS Dissertations and Theses

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Molecular Determinants Of Glut1: Structure And Function: A Dissertation, Ralph J. Zottola Jun 1994

Molecular Determinants Of Glut1: Structure And Function: A Dissertation, Ralph J. Zottola

GSBS Dissertations and Theses

Hebert and Carruthers (1992) showed that the human erythrocyte glucose transporter is an allosteric complex of four GLUT1 proteins whose structure and substrate binding properties are stabilized by reductant-sensitive noncovalent subunit interactions. The GLUT1 tetramer dissociates into dimers upon exposure to reductant but subunits are not associated via disulfide bridges. Each subunit of SDS-denatured tetrameric GLUT1 exposes only two thiols while reduced denatured GLUT1 exposes all six sulfhydryl groups. They hypothesized that glucose transporter oligomeric structure and cooperative catalytic function resulted from noncovalent subunit interactions promoted or stabilized by intramolecular disulfide bridges. These interactions give rise to an antiparallel arrangement ...