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Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

1994

Botany Publication and Papers

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Molecular Cloning Of Cdnas And Genes Coding For Beta-Methylcrotonyl-Coa Carboxylase Of Tomato, Xun Wang, Eve S. Wurtele, Greg Keller, Angela L. Mckean, Basil J. Nikolau Jan 1994

Molecular Cloning Of Cdnas And Genes Coding For Beta-Methylcrotonyl-Coa Carboxylase Of Tomato, Xun Wang, Eve S. Wurtele, Greg Keller, Angela L. Mckean, Basil J. Nikolau

Botany Publication and Papers

Tomato cDNA and genomic clones were isolated by using as a probe a cDNA clone that had originally been identified by its ability to direct the synthesis of a biotin-containing polypeptide in Escherichia coli. The nucleotide sequences of the newly isolated cDNAs indicate that they are clones of a single mRNA molecule. However, one of the cDNA clones contains an insertion of a sequence which we identified as an unspliced intron. The amino acid sequence deduced from the nucleotide sequence of the cDNAs showed similarity to regions of previously sequenced biotin enzymes, indicating that the isolated cDNAs code for a ...