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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Development Of An Assay To Determine The Amount Of Binding Between Dna And The Carcinogen (+)-Anti-Benzo (A) Pyrene Diol Eopxide, Cher Wright Apr 1994

Development Of An Assay To Determine The Amount Of Binding Between Dna And The Carcinogen (+)-Anti-Benzo (A) Pyrene Diol Eopxide, Cher Wright

Life and Environmental Sciences Undergraduate Theses

A method for determining the number of carcinogenic molecules bound (adducted) to a DNA sequence was studied. Samples of purified plasmid DNA were exposed to increasing concentrations of the carcinogenic chemical benzo(a)pyrene diol epoxide (BPDE). Treated plasmids then served as the template in a Polymerase Chain Reaction (PCR). Desired test regions of the plasmid were selectively amplified. Samples from the PCR were then electrophoresed to study the number of successful copies produced by the PCR and the size and density of the bands. DNA replication levels decreased proportionately as BPDE exposure concentrations increased. The results suggest that the ...


Rt-Pcr Analysis Of Prn-P Mrna Expression In Normal And Scrapie-Infected B6.1(Prn-Pb) . B6(Prn-Pa). B6xb6.1(Prn-Pa^B) , Tgl5 (Prn-P2a/3b) And Prn-Pablated Mice, Bradley Graham Apr 1994

Rt-Pcr Analysis Of Prn-P Mrna Expression In Normal And Scrapie-Infected B6.1(Prn-Pb) . B6(Prn-Pa). B6xb6.1(Prn-Pa^B) , Tgl5 (Prn-P2a/3b) And Prn-Pablated Mice, Bradley Graham

Life and Environmental Sciences Undergraduate Theses

Brains and spleens were extracted from B6.I, B6, FI, Tgl5, and ablated strains of mice, both normal and scrapieinfected. Each strain exhibits a different incubation time due to slight differences found in the allele coding for Prnp mRNA. Total RNA was isolated from these organs. Absorbance readings at 260 nm and 280 nm were used to calculate the concentration of total RNA for each tissue. The reverse transcriptase polymerase chain reaction (RTPCR) was then used to identify different Prn-p alleles and to determine if a quantitative difference in Prn-p mRNA expression exists in the aforementioned mouse strains. Successful distinction ...