Open Access. Powered by Scholars. Published by Universities.®

Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 9 of 9

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Circadian Clock Locus Frequency: Protein Encoded By A Single Open Reading Frame Defines Period Length And Temperature Compensation., Benjamin D. Aronson, Keith A. Johnson, Jay C. Dunlap Aug 1994

Circadian Clock Locus Frequency: Protein Encoded By A Single Open Reading Frame Defines Period Length And Temperature Compensation., Benjamin D. Aronson, Keith A. Johnson, Jay C. Dunlap

Open Dartmouth: Faculty Open Access Scholarship

The frequency (frq) locus encodes a key component, a state variable, in a cellular oscillator generating circadian rhythmicity. Two transcripts have been mapped to this region, and data presented here are consistent with the existence of a third transcript. Analysis of cDNA clones and clock mutants from this region focuses attention on one transcript encoding a protein. FRQ, which is a central clock component: (i) mutations in all of the semidominant frq alleles are the result of single amino acid substitutions and map to the open reading frame (ORF) encoding FRQ; (ii) deletion of this ORF, or a frameshift mutation ...


Development Of An Assay To Determine The Amount Of Binding Between Dna And The Carcinogen (+)-Anti-Benzo (A) Pyrene Diol Eopxide, Cher Wright Apr 1994

Development Of An Assay To Determine The Amount Of Binding Between Dna And The Carcinogen (+)-Anti-Benzo (A) Pyrene Diol Eopxide, Cher Wright

Life and Environmental Sciences Undergraduate Theses

A method for determining the number of carcinogenic molecules bound (adducted) to a DNA sequence was studied. Samples of purified plasmid DNA were exposed to increasing concentrations of the carcinogenic chemical benzo(a)pyrene diol epoxide (BPDE). Treated plasmids then served as the template in a Polymerase Chain Reaction (PCR). Desired test regions of the plasmid were selectively amplified. Samples from the PCR were then electrophoresed to study the number of successful copies produced by the PCR and the size and density of the bands. DNA replication levels decreased proportionately as BPDE exposure concentrations increased. The results suggest that the ...


Rt-Pcr Analysis Of Prn-P Mrna Expression In Normal And Scrapie-Infected B6.1(Prn-Pb) . B6(Prn-Pa). B6xb6.1(Prn-Pa^B) , Tgl5 (Prn-P2a/3b) And Prn-Pablated Mice, Bradley Graham Apr 1994

Rt-Pcr Analysis Of Prn-P Mrna Expression In Normal And Scrapie-Infected B6.1(Prn-Pb) . B6(Prn-Pa). B6xb6.1(Prn-Pa^B) , Tgl5 (Prn-P2a/3b) And Prn-Pablated Mice, Bradley Graham

Life and Environmental Sciences Undergraduate Theses

Brains and spleens were extracted from B6.I, B6, FI, Tgl5, and ablated strains of mice, both normal and scrapieinfected. Each strain exhibits a different incubation time due to slight differences found in the allele coding for Prnp mRNA. Total RNA was isolated from these organs. Absorbance readings at 260 nm and 280 nm were used to calculate the concentration of total RNA for each tissue. The reverse transcriptase polymerase chain reaction (RTPCR) was then used to identify different Prn-p alleles and to determine if a quantitative difference in Prn-p mRNA expression exists in the aforementioned mouse strains. Successful distinction ...


Molecular Cloning Of Cdnas And Genes Coding For Beta-Methylcrotonyl-Coa Carboxylase Of Tomato, Xun Wang, Eve S. Wurtele, Greg Keller, Angela L. Mckean, Basil J. Nikolau Jan 1994

Molecular Cloning Of Cdnas And Genes Coding For Beta-Methylcrotonyl-Coa Carboxylase Of Tomato, Xun Wang, Eve S. Wurtele, Greg Keller, Angela L. Mckean, Basil J. Nikolau

Botany Publication and Papers

Tomato cDNA and genomic clones were isolated by using as a probe a cDNA clone that had originally been identified by its ability to direct the synthesis of a biotin-containing polypeptide in Escherichia coli. The nucleotide sequences of the newly isolated cDNAs indicate that they are clones of a single mRNA molecule. However, one of the cDNA clones contains an insertion of a sequence which we identified as an unspliced intron. The amino acid sequence deduced from the nucleotide sequence of the cDNAs showed similarity to regions of previously sequenced biotin enzymes, indicating that the isolated cDNAs code for a ...


Telomeric Nucleic Acids: C-Strand Structure And A Telomerase Rna Mutant , Shawn Cameron Ahmed Jan 1994

Telomeric Nucleic Acids: C-Strand Structure And A Telomerase Rna Mutant , Shawn Cameron Ahmed

Retrospective Theses and Dissertations

Telomeres, the ends of linear chromosomes, are composed of simple tandem repeats which are usually G·C rich. Telomeres are essential for chromosome stability, organizing the nuclear architecture and ensuring complete replication of the chromosomal terminus. To understand how telomeres carry out these fundamental cellular roles, one must understand the structural and dynamic properties of telomeric repeat sequences. Structural and genetic approaches were taken to learn more about telomeric nucleic acids;The structural portion of my research concerned an unusual DNA structure formed by the C-rich strand of telomeric DNA. Telomeric C-strand sequences form non-Watson-Crick structures in supercoiled plasmids at ...


Global Phylogeography Of Yellowfin Tuna, Thunnus Albacares, And Mackerels Of The Genus Scomber, Daniel R. Scoles Jan 1994

Global Phylogeography Of Yellowfin Tuna, Thunnus Albacares, And Mackerels Of The Genus Scomber, Daniel R. Scoles

Dissertations, Theses, and Masters Projects

Intraspecific genetic relationships within yellowfin tuna, Thunnus albacares, and three mackerels of the genus Scomber were studied by restriction fragment length polymorphism (RFLP) analysis of mitochondrial DNA (mtDNA). The comparison of these scombrids, with different larval distributions, adult distributions, and vagilities, served to investigate the hypothesis that population structure in marine fishes results from geographic and physical oceanographic processes that limit dispersal of early life history stages. Samples of 20 yellowfin tuna were examined from each of five Pacific locations and one Atlantic location. MtDNA analysis with 12 informative restriction endonucleases demonstrated considerable genetic variation, as evidenced by overall nucleon ...


Genetic Analysis Of Pseudohyphal Growth In The Budding Yeast Saccharomyces Cerevisiae , Melissa Jo Blacketer Jan 1994

Genetic Analysis Of Pseudohyphal Growth In The Budding Yeast Saccharomyces Cerevisiae , Melissa Jo Blacketer

Retrospective Theses and Dissertations

The budding yeast Saccharomyces cerevisiae is a dimorphic organism that can assume either a yeast-like or pseudohyphal form. Nitrogen limitation induces pseudohyphal growth, which is characterized by branched chains of elongated cells. Pseudohyphal cells can grow invasively in agar medium, whereas yeast-like cells do not. To identify factors involved in morphologic differentiation, S. cerevisiae mutants exhibiting a constitutive cell elongation morphology were isolated. Genetic analysis identified 28 recessive and 2 semi-dominant mutations that cause abnormal morphology, and placed these in 14 distinct gene loci, termed ELM, for ELongated Morphology. Many elm mutations cause multiple aspects of pseudohyphal growth, and thus ...


Soybean Cultivar Development: A Genome Perspective , Lisa Lou Lorenzen Jan 1994

Soybean Cultivar Development: A Genome Perspective , Lisa Lou Lorenzen

Retrospective Theses and Dissertations

The purpose of this dissertation was to retrospectively analyze the process of soybean cultivar development from a molecular perspective, by following the inheritance of RFLP markers in a pedigree analysis. Five methods of analysis are included in this dissertation;Sixty-four plant introductions, cultivars, and breeding lines were analyzed to define a core set of 97 probes with a high likelihood of detecting a polymorphism in elite germplasm. The ability to trace regions of chromosomes from parent to offspring and the application of pedigree-based mapping was demonstrated, which is the cosegregation of phenotypic traits and RFLP markers based on pedigree information ...


An Analysis Of Mitochondrial Dna In Rett Syndrome And Other Neurodegenerative Disorders, Catherine Erickson Burgess Jan 1994

An Analysis Of Mitochondrial Dna In Rett Syndrome And Other Neurodegenerative Disorders, Catherine Erickson Burgess

Theses and Dissertations in Biomedical Sciences

Mitochondrial dysfunction resulting from mutations on mitochondrial DNA (mtDNA) is being recognized in a growing spectrum of diseases. These diseases, resulting from single base mutations, large deletions, or insertions, have been largely neuromuscular in origin. However, as an understanding of the effects of mtDNA mutations progresses, attention is now focusing on neurodegenerative diseases. Rett Syndrome (RS), a progressive neurodegenerative disease with predominantly female cases, demonstrates morphologic mitochondrial changes, mitochondrial enzyme deficiencies and maternal inheritance (characteristic of mtDNA diseases). No investigation of mtDNA involvement has been previously conducted and, to date, no biological marker exists for this disorder.

Our preliminary studies ...