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1992

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Articles 1 - 30 of 61

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Purification And Ligand Binding Of A Soluble Class I Mhc Molecule Consisting Of The First Three Domains Of H-2kd Fused To B2-Microglobulin Expressed In The Baculovirus/Insect Cell System, Francois Godeau, Immanuel F. Luescher, David M. Ojcius, Cecile Saucier, Estelle Mottez, Lucien Cabanie, Philippe Kourilsky Dec 1992

Purification And Ligand Binding Of A Soluble Class I Mhc Molecule Consisting Of The First Three Domains Of H-2kd Fused To B2-Microglobulin Expressed In The Baculovirus/Insect Cell System, Francois Godeau, Immanuel F. Luescher, David M. Ojcius, Cecile Saucier, Estelle Mottez, Lucien Cabanie, Philippe Kourilsky

Dugoni School of Dentistry Faculty Articles

A recombinant baculovirus encoding a single-chain murine major histocompatibility complex class I molecule in which the first three domains of H-2Kd are fused to beta 2-microglobulin (beta 2-m) via a 15-amino acid linker has been isolated and used to infect lepidopteran cells. A soluble, 391-amino acid single-chain H-2Kd (SC-Kd) molecule of 48 kDa was synthesized and glycosylated in insect cells and could be purified in the absence of detergents by affinity chromatography using the anti-H-2Kd monoclonal antibody SF1.1.1.1. We tested the ability of SC-Kd to bind antigenic peptides using a direct binding assay based on photoaffinity labeling ...


Isolation And Characterization Of The Tricarboxylate Transporter From Pea Mitochondria, Cecilia A. Mcintosh, David J. Oliver Dec 1992

Isolation And Characterization Of The Tricarboxylate Transporter From Pea Mitochondria, Cecilia A. Mcintosh, David J. Oliver

David J. Oliver

The tricarboxylate transporter was solubilized from pea (Pisum sativum) mitochondria with Triton X-114, partially purified over a hydroxylapatite column, and reconstituted in phospholipid vesicles. The proteoliposomes exchanged external [14C]citrate for internal citrate or malate but not for preloaded D,L-isocitrate. Similarly, although external malate, succinate, and citrate competed with [14C]citrate in the exchange reaction, D,L-isocitrate and phosphoenolpyruvate did not. This tricarboxylate transporter differed from the equivalent activity from animal tissues in that it did not transport isocitrate and phosphoenolpyruvate. In addition, tricarboxylate transport in isolated plant mitochondria, as well as that measured with the partially purified and ...


Identification Of The Nd Δ And Σ States And The 1,3Φ←←X  3Σ− G Transition Of O2 By Resonant Multiphoton Ionization, Robert J. Yokelson, R. J. Lipert, W. A. Chupka Nov 1992

Identification Of The Nd Δ And Σ States And The 1,3Φ←←X  3Σ− G Transition Of O2 By Resonant Multiphoton Ionization, Robert J. Yokelson, R. J. Lipert, W. A. Chupka

Chemistry and Biochemistry Faculty Publications

Spectra of the 3dRydberg state region of O2 have been obtained by two‐photon resonant ionization of the ground electronic state. By varying the rotational distribution and radiation polarization, all observed bands were identified and attributed to excitation of Σ, Δ, and Φ states. Earlier assignments were corrected. The Δ and Φ assignments are complete while the Σ assignments are so far incomplete.


Identification Of The Ns And Nd Rydberg States Of O2 For N=3–5, Robert J. Yokelson, R. J. Lipert, W. A. Chupka Nov 1992

Identification Of The Ns And Nd Rydberg States Of O2 For N=3–5, Robert J. Yokelson, R. J. Lipert, W. A. Chupka

Chemistry and Biochemistry Faculty Publications

The 4s‐3d and 5s‐4dRydberg complexes of diatomic oxygen have been studied by (2+1) resonance‐enhanced multiphoton ionization of the X3 g ground state of O2. We have located and identified at least two vibrational levels of each of the following states: Three of four expected 4sσ Π states; all four expected 5sσ Π states; 18 of 22 expected 3d states (with only the states of the 3dσ orbital remaining unobserved); and 5 of the 10 predicted 4dπ states. State assignments were ...


Circular Dichroism And Molecular Modeling Yield A Structure For The Complex Of Human Immunodeficiency Virus Type 1 Trans-Activation Response Rna And The Binding Region Of Tat, The Trans-Acting Transcriptional Activator, Erwann P. Loret, Philippe T. Georgel, W. Curtis Johnson Jr., Pui Shing Ho Oct 1992

Circular Dichroism And Molecular Modeling Yield A Structure For The Complex Of Human Immunodeficiency Virus Type 1 Trans-Activation Response Rna And The Binding Region Of Tat, The Trans-Acting Transcriptional Activator, Erwann P. Loret, Philippe T. Georgel, W. Curtis Johnson Jr., Pui Shing Ho

Biological Sciences Faculty Research

Transcription in the human immunodeficiency virus type 1 (HIV-1) retrovirus is regulated by binding the viral Tat protein (trans-acting transcriptional activator) to the trans-activation response (TAR) RNA sequence. Here, vacuum UV circular dichroism (VUV-CD) is used to study the structure of TAR and its complex with two peptide fragments that are important for Tat binding to TAR. The VUV-CD spectrum of TAR is typical of A-form RNA and is minimally perturbed when bound to either the short or the long Tat peptide. The CD spectra ofthe complexes indicate an extended structure in the argnine-rich region of Tat from amino acid ...


Thermodynamics Of Single-Standard Rna-Binding To Oligolysines Containing Tryptophan, David Mascotti, Timothy Lohman Sep 1992

Thermodynamics Of Single-Standard Rna-Binding To Oligolysines Containing Tryptophan, David Mascotti, Timothy Lohman

David P. Mascotti

These studies provide important comparative information for the interpretation of the thermodynamics of protein-ss nucleic acid interaction.


The Structure Of A Complex Of Bovine &-Thrombin And Recombinant Hirudin At 2.8-A Resolution, Jacqueline Vitali, Philip D. Martin, Michael G. Malkowski, William D. Robertson, Jerome B. Lazar, Richard C. Winant, Paul H. Johnson, Brian F.P. Edwards Sep 1992

The Structure Of A Complex Of Bovine &-Thrombin And Recombinant Hirudin At 2.8-A Resolution, Jacqueline Vitali, Philip D. Martin, Michael G. Malkowski, William D. Robertson, Jerome B. Lazar, Richard C. Winant, Paul H. Johnson, Brian F.P. Edwards

Physics Faculty Publications

Crystals of the complex of bovine alpha-thrombin with recombinant hirudin variant 1 have space group C222(1) with cell constants a = 59.11, b = 102.62, and c = 143.26 A. The orientation and position of the thrombin component was determined by molecular replacement and the hirudin molecule was fit in 2 magnitude of Fo - magnitude of Fc electron density maps. The structure was refined by restrained least squares and simulated annealing to R = 0.161 at 2.8-A resolution. The binding of hirudin to thrombin is generally similar to that observed in the crystals of human thrombin-hirudin. Several differences ...


Nad+-Linked Isocitrate Dehydrogenase: Isolation, Purification, And Characterization Of The Protein From Pea Mitochondria, Cecilia A. Mcintosh, David J. Oliver Sep 1992

Nad+-Linked Isocitrate Dehydrogenase: Isolation, Purification, And Characterization Of The Protein From Pea Mitochondria, Cecilia A. Mcintosh, David J. Oliver

David J. Oliver

The NAD+-dependent isocitrate dehydrogenase from etiolated pea (Pisum sativum L.) mitochondria was purified more than 200-fold by dye-ligand binding on Matrix Gel Blue A and gel filtration on Superose 6. The enzyme was stabilized during purification by the inclusion of 20% glycerol. In crude matrix extracts, the enzyme activity eluted from Superose 6 with apparent molecular masses of 1400 ± 200, 690 ± 90, and 300 ± 50 kD. During subsequent purification steps the larger molecular mass species disappeared and an additional peak at 94 ± 16 kD was evident. The monomer for the enzyme was tentatively identified at 47 kD by sodium ...


The Cpce And Cpcf Genes Of Synechococcus Sp. Pcc 7002. Construction And Phenotypic Characterization Of Interposon Mutants, J Zhou, G E. Gasparich, V L. Stirewalt, R De Lorimier, D A. Bryant Aug 1992

The Cpce And Cpcf Genes Of Synechococcus Sp. Pcc 7002. Construction And Phenotypic Characterization Of Interposon Mutants, J Zhou, G E. Gasparich, V L. Stirewalt, R De Lorimier, D A. Bryant

Gail Gasparich

The 3' region of the cpc operon of Synechococcus sp. PCC 7002 has been sequenced, transcriptionally characterized, and analyzed by interposon mutagenesis. The cpc operon contains six genes, 5' cpcB-cpcA-cpcC-cpcD-cpcE-cpcF 3', and gives rise to at least eight (more likely ten) discrete mRNA transcripts. The steady-state levels of transcripts for the cpcE and cpcF genes are very low and are estimated to represent only about 1-2% of the total transcripts arising from the cpc locus. The cpcE gene predicts a protein of 268 amino acid residues, whereas the cpcF gene predicts a protein of 205 amino acid residues. The deduced ...


Appearance Of A Methotrexate Binding Plasma Protein During High Dose Methotrexate Therapy, M P. Iqbal, I Malik, N Mehboobali Aug 1992

Appearance Of A Methotrexate Binding Plasma Protein During High Dose Methotrexate Therapy, M P. Iqbal, I Malik, N Mehboobali

Department of Biological & Biomedical Sciences

No abstract provided.


Saccharomyces Cerevisiae Sec59 Cells Are Deficient In Dolichol Kinase Activity, Loree Heller, Peter Orlean, W. Lee Adair Jr. Aug 1992

Saccharomyces Cerevisiae Sec59 Cells Are Deficient In Dolichol Kinase Activity, Loree Heller, Peter Orlean, W. Lee Adair Jr.

Bioelectrics Publications

The temperature-sensitive Saccharomyces cerevisiae mutant sec59 accumulates inactive and incompletely glycosylated protein precursors in its endoplasmic reticulum at the restrictive temperature. O-mannosylation and glycosyl phosphatidylinositol membrane anchoring of protein are also abolished, consistent with a deficiency in dolichyl phosphate mannose. Membranes prepared from sec59 cells that had been shifted to the restrictive temperature, however, made normal amounts of dolichyl phosphate mannose when exogenous dolichyl phosphate was supplied, but dolichyl phosphate mannose synthesis was severely depressed in the absence of exogenous dolichyl phosphate. Quantitative measurements of dolichyl phosphate in sec59 cells showed that the levels were decreased to 48% of wild ...


Isolation And Characterization Of The Triiodothyronine Regulatory Region Of The Fatty Acid Synthase Gene In Avian Species, Delfina Rivera Valencia Aug 1992

Isolation And Characterization Of The Triiodothyronine Regulatory Region Of The Fatty Acid Synthase Gene In Avian Species, Delfina Rivera Valencia

Master's Theses

Fatty acids are utilized for energy storage in the form of triglycerides or neutral fats. One way to regulate fatty acid biosynthesis is to regulate the activity of fatty acid synthase (FAS), the enzyme that catalyzes the reaction. Previous studies have shown that regulation of FAS activity and synthesis is regulated by the hormone triiodothyronine, T3, at the level of transcription. Thus, isolation and characterization of the promoter region o f the FAS gene will enable the identification of the thyroid hormone response elements (TREs). This study could shed light on the mechanisms on how this gene can be ...


Harvest: A Generalized Animal Population Growth Simulation, David Stewart, Charles Amlaner, Claude Barnett Jun 1992

Harvest: A Generalized Animal Population Growth Simulation, David Stewart, Charles Amlaner, Claude Barnett

Charles J. Amlaner

We present a general purpose discrete-event, stochastic simulation model (HARVEST) which can be used to model the population growth of a wide variety of animal species. A complete example is given with validation, to show how it can be used for managing the Eastern wild turkey. The structure of the simulation system (micro Passim) is described, and the implementation of the model is discussed as an example of how the process-interaction simulation protocol may be used to represent a complex natural system. The model is designed to be used by state and federal wildlife agencies for exploration of the effects ...


Differential Regulation Of Collagenase Gene Expression By Retinoic Acid Receptors--Alpha, Beta And Gamma, Luying Pan, Stephen H. Chamberlain, David T. Auble, Constance E. Brinckerhoff Jun 1992

Differential Regulation Of Collagenase Gene Expression By Retinoic Acid Receptors--Alpha, Beta And Gamma, Luying Pan, Stephen H. Chamberlain, David T. Auble, Constance E. Brinckerhoff

Open Dartmouth: Faculty Open Access Articles

The mechanisms involved in retinoic acid (RA)-mediated regulation of the collagenase gene in a rabbit synovial fibroblast cell line (HIG82) were investigated. When HIG82 cells are cotransfected with expression vectors containing cDNAs for retinoic acid receptor (RAR) α1, β2, or γ1 and collagenase promoter-driven CAT reporter constructs, only RAR-γ1 represses basal CAT expression upon RA treatment, while RAR-α1, β2, and γ1 all suppress phorbol-induced CAT expression. Thus, transcriptional regulation of collagenase by RA is mediated by RARs in an RAR-type specific manner. Using mutatlonal and deletional analysis, we find that interaction between elements within 182 bp collagenase promoter plays ...


Distinct Permissive Pathways Mediate The Effects Of Nerve Growth Factor And Lithium On Neurotensin/Neuromedin N Gene Expression In Pc12 Cells: A Thesis, Bryant Paul Bullock Jun 1992

Distinct Permissive Pathways Mediate The Effects Of Nerve Growth Factor And Lithium On Neurotensin/Neuromedin N Gene Expression In Pc12 Cells: A Thesis, Bryant Paul Bullock

GSBS Dissertations and Theses

This thesis examines the effects of nerve growth factor (NGF) and lithium on the regulation of neurotensin/neuromedin N (NT/N) gene expression in PC12 pheochromocytoma cells. In PC12 cells, the expression of the rat NT/N gene is strictly dependent on simultaneous exposure to combinations of NGF, glucocorticoids, activators of adenylate cyclase, and lithium. Transient transfection experiments indicated that a consensus AP-1 site located within the NT/N promoter is the principal target of NGF and lithium action. NGF rapidly, but transiently, induces the expression of several AP-1 genes in PC12 cells, suggesting that the effect of NGF on ...


Evidence For Helical Structures In Poly(1-Olefin Sulfones) By Transmission Electron Microscopy, George C. Ruben, W H. Stockmayer May 1992

Evidence For Helical Structures In Poly(1-Olefin Sulfones) By Transmission Electron Microscopy, George C. Ruben, W H. Stockmayer

Open Dartmouth: Faculty Open Access Articles

Transmission electron microscope images were obtained of fractions of poly(1-tetradecene sulfone) and poly(cyclohexene sulfone) cast from very dilute solutions (0.007%, wt/vol) and rapidly freeze-dried on a mica surface. The samples were then vertically platinum-carbon (Pt-C) replicated with 9 +/- 0.3-A Pt-C and held together with 128 A of electron-transparent evaporated carbon. The Pt-C coating enlarges the molecular chain diameters by approximately 5 A, so that a single polysulfone chain has an apparent diameter of 9-12 A in the transmission electron microscope. Poly(1-tetradecene sulfone) forms short helical regions that show irregular helical turns of pitch 7-18 ...


Protein Translocation Across The Er Requires A Functional Gtp Binding Site In The Alpha Subunit Of The Signal Recognition Particle Receptor, Peter J. Rapiejko, Reid Gilmore May 1992

Protein Translocation Across The Er Requires A Functional Gtp Binding Site In The Alpha Subunit Of The Signal Recognition Particle Receptor, Peter J. Rapiejko, Reid Gilmore

Open Access Articles

The signal recognition particle (SRP)-mediated translocation of proteins across the RER is a GTP dependent process. Analysis of the primary amino acid sequence of one protein subunit of SRP (SRP54), as well as the alpha subunit of the SRP receptor (SR alpha), has indicated that these proteins contain predicted GTP binding sites. Several point mutations confined to the GTP binding consensus elements of SR alpha were constructed by site specific mutagenesis to define a role for the GTP binding site in SR alpha during protein translocation. The SR alpha mutants were analyzed using an in vitro system wherein SR ...


Mobilization Of Iron Enhances The Iron-Dependent Biochemical Reactivity Of Asbestos In Vitro And Contributes To The Cytotoxicity Of Asbestos In Cultured Cells, Loren Glen Lund May 1992

Mobilization Of Iron Enhances The Iron-Dependent Biochemical Reactivity Of Asbestos In Vitro And Contributes To The Cytotoxicity Of Asbestos In Cultured Cells, Loren Glen Lund

All Graduate Theses and Dissertations

Asbestos related research began approximately 60 years ago, yet, the mechanism(s) by which asbestos exerts its biological effects is not well understood. The hypothesis upon which this dissertation is based is that mobilization of iron from asbestos enhances the iron-dependent biochemical reactivity of asbestos in vitro and contributes to asbestos-dependent cytotoxicity. The specific aims for this hypothesis were, 1) to determine whether iron was responsible for the biochemical reactivity of asbestos in vitro and asbestos-induced cytotoxicity in cultured cells, and 2) to determine whether mobilization of iron from asbestos enhanced the reactions catalyzed by asbestos in vitro and contributes ...


Methotrexate As An Alternative To Surgery In Ectopic Pregnancy: A New Role For An Old Drug, M P. Iqbal Apr 1992

Methotrexate As An Alternative To Surgery In Ectopic Pregnancy: A New Role For An Old Drug, M P. Iqbal

Department of Biological & Biomedical Sciences

Abstract is not provided by the author/publisher


H-Protein Of The Glycine Decarboxylase Multienzyme Complex: Complementary Dna Encoding The Protein From Arabidopsis Thaliana, David J. Oliver Apr 1992

H-Protein Of The Glycine Decarboxylase Multienzyme Complex: Complementary Dna Encoding The Protein From Arabidopsis Thaliana, David J. Oliver

David J. Oliver

The glycine decarboxylase multienzyme complex is composed of four component enzymes, the 100-kD P-protein (a pyridoxal 5-phosphate-dependent amino acid decarboxylase), the 14-kD H-protein (a lipoamide-containing carrier protein), the 45-kD T-protein (a tetrahydrofolate transferase), and the 59-kD L-protein (lipoamide dehydrogenase) (5). The Iipoamide cofactor of the H-protein carries reaction intermediates between the active sites of the other three component proteins. These proteins form a large molecular weight complex (4) within the matrix ofleaf mitochondria where, together with the enzyme serine hydroxymethyltransferase, it catalyzes the photorespiratory conversion of two molecules of glycine to one each of serine, NH3, and CO2 with the ...


Enzyme Cytochemical Localization Of Na,K-Atpase And Immunohistochemical Localization Of Vinculin In Frog And Mouse Liver Tissue, Douglas Mcinnis Apr 1992

Enzyme Cytochemical Localization Of Na,K-Atpase And Immunohistochemical Localization Of Vinculin In Frog And Mouse Liver Tissue, Douglas Mcinnis

Life and Environmental Sciences Undergraduate Theses

Means of localizing molecules are essential in contemporary biological research attempting to unlock the mysteries of how cells function, interact, and go wrong. Enzyme cytochemistry and immunohistochemistry are two methods which utilize different characteristics of given molecules to localize them in tissue. The objective of this study was to localize two proteins, Na,K-ATPase and vinculin, in frog and mouse liver sections using these methods. Na,KATPase activity contributes to overall p-nitrophenylphosphatase (jpNPPase) activity, and sites of p-NPPase activity were determined using an enzyme cytochemical method. Sites of p-NPPase activity were thus extrapolated to indicate regions of probable Na,K-ATPase ...


In Vitro Study Of The Effect Of Hypothermia On The Dissociation Curve Of Blood, Matthew Munding Apr 1992

In Vitro Study Of The Effect Of Hypothermia On The Dissociation Curve Of Blood, Matthew Munding

Life and Environmental Sciences Undergraduate Theses

The aim of this study was to examine the change in the dissociation curve of oxygen from human hemoglobin at the temperatures of 37°, 25°, 18°, 12°, 7°, and 3° C. The partial pressures of oxygen and the calculated hemoglobin saturations were determined for samples at the various temperatures. From this data the partial pressures at 75% saturation were calculated, and the differences in the partial pressures at 75% saturation were compared. The dissociation curve was shown to shift to the left as the temperature was decreased down to 12°. Below 12°, the shift was not further observed. At the ...


Estimation And Separation Of N-Acetyl-Beta-D-Glucosaminidase Isoenzymes In Urine, M H. Javed, A N. Hussain, M A. Waqar Mar 1992

Estimation And Separation Of N-Acetyl-Beta-D-Glucosaminidase Isoenzymes In Urine, M H. Javed, A N. Hussain, M A. Waqar

Department of Biological & Biomedical Sciences

N-acetyl-beta-D-glucosaminidase is a lysosomal enzyme made up of two isoenzymes (A and B). It has been used extensively as a marker for kidney damage. However, its estimation in urine has not been standardized. We have established a method for the estimation and separation of NAG isoenzymes by ion-exchange chromatography. In 19 experiments done so far, this method has given reproducible results. The significance of this method is that with a single experiment, one can estimate total as well as individual isoenzyme activity. Furthermore, urine constituents do not appear to interfere in this assay.


Interaction Of Single And Double Stranded Nucleic Acids With Charged Oligopeptides Containing Tryptophan, David Mascotti, T. Lohman Jan 1992

Interaction Of Single And Double Stranded Nucleic Acids With Charged Oligopeptides Containing Tryptophan, David Mascotti, T. Lohman

David P. Mascotti

No abstract provided.


Association Of Tetrapyrrole Intermediates In The Bacteriochlorophyll A Biosynthetic Pathway With The Major Outer-Membrane Porin Protein Of Rhodobacter Capsulatus, David Bollivar Jan 1992

Association Of Tetrapyrrole Intermediates In The Bacteriochlorophyll A Biosynthetic Pathway With The Major Outer-Membrane Porin Protein Of Rhodobacter Capsulatus, David Bollivar

Scholarship

No abstract provided.


Nucleotide Sequence Of S-Adenosyl-L-Methionine: Magnesium Protoporphyrin Methyltransferase From Rhodobacter Capsulatus, David Bollivar Jan 1992

Nucleotide Sequence Of S-Adenosyl-L-Methionine: Magnesium Protoporphyrin Methyltransferase From Rhodobacter Capsulatus, David Bollivar

Scholarship

No abstract provided.


The Characterization And Purification Of Matrixin-A, A Plasmin Releasable Growth Inhibitor Associated With The Extracellular Matrix , Scott William Miller Jan 1992

The Characterization And Purification Of Matrixin-A, A Plasmin Releasable Growth Inhibitor Associated With The Extracellular Matrix , Scott William Miller

Retrospective Theses and Dissertations

The aim of this project is to examine the role of proteases and protease inhibitors in the regulation of cell proliferation. It also describes a simple, high yield purification of bovine platelet derived TGF-[beta] that uses a novel bioassay to specifically identify TGF-[beta] mediated activity;The composition of the extracellular matrix (ECM) is an important determinant of the rate cellular proliferation and differentiation. Growth factors and other proteins with growth regulatory activities can affect the composition of the ECM. Growth regulators are also associated with the ECM and can be released by the action of ECM associated proteolysis ...


Improved Recovery Of A Radlolabeled Peptide With An Albumin-Treated Reversed-Phase Hplc Column, David S. Hage, Robert L. Taylor, Pai C. Kao Jan 1992

Improved Recovery Of A Radlolabeled Peptide With An Albumin-Treated Reversed-Phase Hplc Column, David S. Hage, Robert L. Taylor, Pai C. Kao

David Hage Publications

Reversed-phase high-performance liquid chromatography (RP-HPLC) is an important tool in the purification of radiolabeled peptides and proteins for immunoassay. However, for some proteins and peptides it is difficult to achieve reproducible behavior in RP-HPLC because of the low recovery of these compounds. Factors that can be varied to improve recovery include the strength or pH of the mobile phase, the chain length and spacing of groups on the reversed-phase support, and the flow rate or steepness of the elution gradient (1-5). ... In summary, we obtained better recovery and more reproducible chromatographic behavior for labeled 1-34 PTHrP with an albumin-pretreated reversed-phase ...


Intact Parathyroid Hormone: Performance And Clinical Utility Of An Automated Assay Based On High-Performance Immunoaffinity Chromatography And Chemiluminescence Detection, David S. Hage, Bob Taylor, Pai C. Kao Jan 1992

Intact Parathyroid Hormone: Performance And Clinical Utility Of An Automated Assay Based On High-Performance Immunoaffinity Chromatography And Chemiluminescence Detection, David S. Hage, Bob Taylor, Pai C. Kao

David Hage Publications

The performance and clinical utility of an automated assay of intact parathyroid hormone (parathyrin, PTH) are evaluated. The method is based on the extraction of PTH from plasma by an HPLC column containing immobilized anti-(44-68 PTH) antibodies. The PTH retained is detected with a postcolumn reactor and use of anti-(1--34 PTH) chemiluminescent-labeled antibodies. The total cycle time of the assay is 6.5 mm per injection after a 1-h incubation.The lower limit of detection for PTH in a 66-pL plasma sample was 0.5 pmol/L based on peak heights and 0.2 pmol/L based on ...


Translational Frameshifting By Barley Yellow Dwarf Virus Rna , Rong Di Jan 1992

Translational Frameshifting By Barley Yellow Dwarf Virus Rna , Rong Di

Retrospective Theses and Dissertations

Barley yellow dwarf virus is a single-stranded RNA virus, consisting of several serotypes including the PAV serotype which is the focus of this dissertation. The 39K open reading frame (ORF) at the 5[superscript]' end of the 5.7 kb (kilobase) genome overlaps with a downstream 60K ORF by 13 nucleotides. Several approaches were employed to demonstrate that the 60K ORF (putative polymerase gene) is expressed by an unusual minus one (-1) translational frameshifting mechanism. A sequence encompassing this overlap region induces frameshifting in heterologous and native contexts. The efficiency of frameshifting induced by the PAV sequence was approximately 3 ...