Open Access. Powered by Scholars. Published by Universities.®

Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 4 of 4

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Avena Phytochrome Mrna: In Vivo Localization, Degradation Products, And Half-Life , Kevin Andrew Seeley Jan 1991

Avena Phytochrome Mrna: In Vivo Localization, Degradation Products, And Half-Life , Kevin Andrew Seeley

Retrospective Theses and Dissertations

The purpose of this research is to determine the role that post-transcriptional processes play in the regulation of phytochrome (phy) steady-state messenger RNA (mRNA) levels. The control of etiolated phytochrome (phyA) and chlorophyll a/b binding protein (cab) gene transcription and steady-state mRNA levels are known to be regulated by the phyA signal transduction pathway. I performed a number of control experiments that indicate the degradation products detected in northern analysis of phyA mRNA were produced in vivo and were not an artifact of RNA isolation techniques. I have also shown that infrared (IR) irradiation has no appreciable effect on ...


Analysis Of Three Plasmids From Filamentous, Nitrogen-Fixing Cyanobacteria , Douglas Kevin Walton Jan 1991

Analysis Of Three Plasmids From Filamentous, Nitrogen-Fixing Cyanobacteria , Douglas Kevin Walton

Retrospective Theses and Dissertations

The cyanobacteria are an ancient and diverse group of prokaryotes capable of oxygenic photosynthesis. They display both unicellular and filamentous cell types and some have the ability to fix atmospheric dinitrogen, and the ability to differentiate specialized cells. In addition, many of the cyanobacteria possess plasmids. To date, only one plasmid, from a unicellular non-nitrogen-fixer, has been characterized in detail. The current lack of information concerning cyanobacterial plasmids, and their subsequent use as vectors, has hindered genetic analysis, particularly in the filamentous species;In this study, the properties of two plasmids and one plasmid origin of replication from filamentous, nitrogen-fixing ...


Effect Of 1,10-Phenanthroline On Dna Binding Of 1,25-Dihydroxyvitamin D Receptors And Characterization And Regulation Of Retinoic Acid Receptors In Rat Osteosarcoma Cells , Kevin Brady Atkins Jan 1991

Effect Of 1,10-Phenanthroline On Dna Binding Of 1,25-Dihydroxyvitamin D Receptors And Characterization And Regulation Of Retinoic Acid Receptors In Rat Osteosarcoma Cells , Kevin Brady Atkins

Retrospective Theses and Dissertations

The effect of the chelator 1,10-phenanthroline on the DNA-binding of 1,25-dihydroxyvitamin D receptor was demonstrated. Increasing concentrations of the chelator, up to 3 mM, decreased receptor binding to DNA-cellulose. This effect was specific for the chelating function because the nonchelating analog, 1,7-phenanthroline, did not inhibit DNA-cellulose binding of the receptor. The cations, Cd[superscript]2+ and Zn[superscript]2+, were able to block chelator-mediated inhibition. These cations were unable to reverse the inhibition unless 1,10-phenanthroline was removed by dialysis;The rat osteosarcoma 17/2.8 cells were shown to express both the cellular retinoic acid binding ...


Purification And Properties Of The Malic Enzyme And The Malate Dehydrogenases From The Gill Tissue Of The Ribbed Mussel (Modiolus Demissus) , Mary Michael Brodey Jan 1991

Purification And Properties Of The Malic Enzyme And The Malate Dehydrogenases From The Gill Tissue Of The Ribbed Mussel (Modiolus Demissus) , Mary Michael Brodey

Retrospective Theses and Dissertations

To assess the role of dicarboxylic acid metabolism in the synthesis of alanine and other compounds by the ribbed mussel under conditions of hyperosmotic and anaerobic stress, the cytosolic and mitochondrial malate dehydrogenases and the mitochondrial malic enzyme from this organism were purified and their kinetics studied. Both the cytosolic and mitochondrial malate dehydrogenases were found to be dimers with native M[subscript] r = 60,000. One cMDH allele (designated slow) was found to predominate in populations from Cape Cod, MA. Similar low K[subscript] m's were observed for purified preparations of the slow/slow homodimer (cMDH) and for ...