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Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

1991

Biochemistry

Pharmacology, Toxicology and Environmental Health

Articles 1 - 2 of 2

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Nitrogen Dioxide Reaction With Proteins: Evidence For Peptide Bond Cleavage At Lysine Residues, Darryl B. Hood May 1991

Nitrogen Dioxide Reaction With Proteins: Evidence For Peptide Bond Cleavage At Lysine Residues, Darryl B. Hood

Electronic Theses and Dissertations

Nitrogen dioxide (NO$\sb2$), an air pollutant produced by burning fossil fuels and a component of cigarette smoke, is thought to contribute to the pathogenesis of pulmonary diseases, such as emphysema. To gain information on the mechanism by which NO$\sb2$ damages the lung, in vitro exposures of $\alpha\sb1$-proteinase inhibitor ($\alpha\sb1$-PI), elastin, bovine serum albumin (BSA), human serum albumin (HSA) and synthetic poly-L-lysine were performed. A genetic deficiency of $\alpha\sb1$-PI predisposes humans to emphysema and NO$\sb2$ has been hypothesized to damage $\alpha\sb1$-PI, which would leave proteases such as human neutrophil elastase ...


Effects Of Oxysterols On Acyl-Coa: Cholesterol Acyltranferase , Meiling Shih Jan 1991

Effects Of Oxysterols On Acyl-Coa: Cholesterol Acyltranferase , Meiling Shih

Retrospective Theses and Dissertations

Two oxysterols, 20(R and S)-20,25-dihydroperoxy-5-cholesten-3[beta]-ol (HPs) isolated from the mixture of cholesterol autoxidation products have been found to reduce effectively cholesteryl ester accumulation in mouse peritoneal macrophages. The effect of HPs was associated with inhibition of cholesterol esterification by suppressing acyl-CoA:cholesterol acyltransferase activity, and by interruption of acetylated low-density-lipoprotein metabolism. The mechanism by which HPs inhibit ACAT activity is investigated in detail in the present study. With reconstituted liposomes, I demonstrated that an increase in cholesterol concentration did not prevent the inhibitory effect of HPs. On the other hand, inactivated ACAT activity was partially ...