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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Mouse Mast Cell Proteases: Induction, Molecular Cloning, And Characterization, Wei Chu May 1991

Mouse Mast Cell Proteases: Induction, Molecular Cloning, And Characterization, Wei Chu

Electronic Theses and Dissertations

Tryptase, a mast cell-specific serine protease with trypsin-like specificity, has been identified in a mouse mast cell line (ABFTL-6) based on it's enzymatic activity, inhibition properties, and cross-reactivity to a human mast cell tryptase antibody. The effects of fibroblast-conditioned medium and sodium butyrate on ABFTL-6 mast cell differentiation and tryptase expression have been examined. ABFTL-6 mouse mast cells undergo phenotypic changes upon culturing in media supplemented with fibroblast-conditioned media at 50% or 1 mM sodium butyrate. The induced cells increased in size, had larger and more metachromatic cytoplasmic granules, and increased their total cellular protein about four-fold. Tryptase activity ...


Characterization Of The Mitogen Regulated Protein Receptor , Joseph T. Nelson Jan 1991

Characterization Of The Mitogen Regulated Protein Receptor , Joseph T. Nelson

Retrospective Theses and Dissertations

Mitogen regulated protein (MRP) is a glycoprotein synthesized in murine placenta and secreted into the maternal bloodstream during gestation. The temporal profile of MRP concentration in the plasma during gestation, its synthesis in an organ known to secrete other lactogenic hormones, and its homology to prolactin suggest an hormonal role;A requisite characteristic of a polypeptide hormone is to have a receptor on the plasma membrane of another tissue separate from its site of synthesis. The receptor then mediates the functional effects by binding the hormone. I discovered and characterized a receptor for MRP from murine uterine membrane preparations. The ...