Open Access. Powered by Scholars. Published by Universities.®

Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

1991

Discipline
Institution
Keyword
Publication
Publication Type
File Type

Articles 1 - 30 of 64

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Retinoic Acid Glucuronide Preparations For Application To The Skin, Arun B. Barua, Desiree Gunning, James A. Olson Dec 1991

Retinoic Acid Glucuronide Preparations For Application To The Skin, Arun B. Barua, Desiree Gunning, James A. Olson

Iowa State University Patents

A method and preparation are described for treating human skin with retinoic acid glucuronide (RAG) in a topical carrier. For treatment of acne or wrinkled skin, RAG can be applied in an effective amount which is nonirritating to the skin. Retinoid dermatitis, an objectionable side effect of topical application of retinoid compounds, can thereby be avoided.


Apparatus And Method For Transient Thermal Infrared Emission Spectrometry, John F. Mcclelland, Roger W. Jones Dec 1991

Apparatus And Method For Transient Thermal Infrared Emission Spectrometry, John F. Mcclelland, Roger W. Jones

Iowa State University Patents

A method and apparatus for enabling analysis of a solid material (16, 42) by applying energy from an energy source (20, 70) top a surface region of the solid material sufficient to cause transient heating in a thin surface layer portion of the solid material (16, 42) so as to enable transient thermal emission of infrared radiation from the thin surface layer portion, and by detecting with a spectrometer/detector (28, 58) substantially only the transient thermal emission of infrared radiation from the thin surface layer portion of the solid material. The detected transient thermal emission of infrared radiation is ...


Apparatus And Method For Transient Thermal Infrared Spectrometry, John F. Mcclelland, Roger W. Jones Dec 1991

Apparatus And Method For Transient Thermal Infrared Spectrometry, John F. Mcclelland, Roger W. Jones

Iowa State University Patents

A method and apparatus for enabling analysis of a material (16, 42) by applying a cooling medium (20, 54) to cool a thin surface layer portion of the material and to transiently generate a temperature differential between the thin surface layer portion and the lower portion of the material sufficient to alter the thermal infrared emission spectrum of the material from the black-body thermal infrared emission spectrum of the material. The altered thermal infrared emission spectrum of the material is detected by a spectrometer/detector (28, 50) while the altered thermal infrared emission spectrum is sufficiently free of self-absorption by ...


The Effects Of Selenate, Molybdate And Chromate On The Triiodothyronine Induced Enzyme Activity And Mrna Level For Both Fatty Acid Synthase And Malic Enzyme, Yuan Zhu Dec 1991

The Effects Of Selenate, Molybdate And Chromate On The Triiodothyronine Induced Enzyme Activity And Mrna Level For Both Fatty Acid Synthase And Malic Enzyme, Yuan Zhu

Master's Theses

In chick embryo hepatocytes in culture, triiodothyronine (T3) stimulates enzyme activity, mRNA level and transcription rate for both fatty acid synthase (FAS) and malic enzyme (ME). Recent evidence has demonstrated the effects of selenate, molybdate and chromate on various physiological processes. Little information, however, is available on the effects of these metal ions on the T3- regulated gene expression for FAS and ME.

In chick embryo hepatocytes incubated in a chemically defined medium, addition of sodium selenate (20 μM) coincident with T3 almost completely inhibited the T3-induced stimulation of FAS and ME activity and accumulation of their respective mRNA's ...


Glucose Transporter Oligomeric Structure Determines The Mechanism Of Glucose Transport: A Dissertation, Daniel N. Hebert Dec 1991

Glucose Transporter Oligomeric Structure Determines The Mechanism Of Glucose Transport: A Dissertation, Daniel N. Hebert

GSBS Dissertations and Theses

The relationship between human erythrocyte glucose transporter (GLUT1) oligomeric structure and function was studied. GLUT1 was purified from human erythrocytes in the absence (GLUT1-DTT) or the presence (GLUT1+DTT) of dithiothreitol. Chemical cross-linking studies of lipid bilayer-resident purified GLUT1 and hydrodynamic studies of cholate-solubilized GLUT1 support the view that GLUT1-DTT is a homotetramer and GLUT1+DTT is a homodimer. Parallel studies on human erythrocyte, and studies employing conformation-specific antibodies (anti-GLUT1-DTT antibodies, ∂-IgGs), indicate that erythrocyte-resident GLUT1 resembles GLUT1-DTT (a homotetramer). While the D-glucose binding capacities of GLUT1-DTT and GLUT1+DTT are indistinguishable, GLUT1-DTT presents at least two population of binding ...


Immunochemical Studies Of Haem Oxygenase. Preparation And Characterization Of Antibodies To Chick Liver Haem Oxygenase And Their Use In Detecting And Quantifying Amounts Of Haem Oxygenase Protein, Yvonne J. Greene, John F. Healey, Herbert L. Bonkovsky Nov 1991

Immunochemical Studies Of Haem Oxygenase. Preparation And Characterization Of Antibodies To Chick Liver Haem Oxygenase And Their Use In Detecting And Quantifying Amounts Of Haem Oxygenase Protein, Yvonne J. Greene, John F. Healey, Herbert L. Bonkovsky

Open Access Articles

Monospecific polyclonal rabbit antibodies to a purified form of haem oxygenase of chick liver, showing sequence similarity to mammalian haem oxygenase-1, were raised and used to study characteristics of the oxygenase. The antibodies inhibited activity of the purified oxygenase, but not other enzyme components (NADPH:cytochrome reductase and biliverdin reductase) of the standard assay mixture of haem oxygenase. In addition, the antibodies inhibited activity of haem oxygenase in microsomes (microsomal fractions) from Cd(2+)-treated chick liver, spleen, testis and brain. Western (immuno-) blots of microsomal proteins of selected organs from chick, rat and man, and homogenates of chick-embryo liver-cell ...


Non-Teratogenic Vitamin A Preparation For Women Of Child-Bearing Age, Arun B. Barua, Desiree Gunning, James A. Olson Oct 1991

Non-Teratogenic Vitamin A Preparation For Women Of Child-Bearing Age, Arun B. Barua, Desiree Gunning, James A. Olson

Iowa State University Patents

For treatment of acne the glucuronide of retinoic acid (retinoyl β-glucuronide) is orally administered to women of child-bearing age who are capable of conception. This therapy is safer than the oral administration of retinoic acid because retinoic acid glucuronide is essentially non-teratogenic at dose levels effective for the acne treatment. The retinoic acid glucuronide can be in all-trans or 13-cis form.


Using Xenon As A Heavy Atom For Determining Phases In Sperm Whale Metmyoglobin, Jacqueline Vitali, Arthur H. Robbins, Steven C. Almo, Robert F. Tilton Oct 1991

Using Xenon As A Heavy Atom For Determining Phases In Sperm Whale Metmyoglobin, Jacqueline Vitali, Arthur H. Robbins, Steven C. Almo, Robert F. Tilton

Physics Faculty Publications

Xenon gas can be used as a heavy atom for determining phases in a protein. We demonstrate that an interpretable electron density map can be obtained for sperm whale metmyoglobin from a single xenon derivative using iterative single isomorphous replacement with the anomalous scattering method.


Characterization Of Two Temperature-Sensitive Mutants Of Escherichia Coli Exhibiting An Altered L22 Ribosomal Protein, Bonnie A. Burnette-Vick Aug 1991

Characterization Of Two Temperature-Sensitive Mutants Of Escherichia Coli Exhibiting An Altered L22 Ribosomal Protein, Bonnie A. Burnette-Vick

Electronic Theses and Dissertations

Analysis of E. coli strains SK1047 and SK1048 have shown them to be temperature-sensitive, protein-synthesis deficient. An alteration in ribosomal protein L22 was detected in both strains using two dimensional gel electrophoresis. Protein L22 was purified from both strains by reversed phase high performance liquid chromatography and from two dimensional electrophoretic gels. Purified ribosomal protein L22 was labeled by reductive methylation and used in 23S RNA binding assays with and without ribosomal protein L4. At the permissive temperature, protein L22 from SK1047 bound less efficiently than the control while protein L22 from SK1048 bound as efficiently as the control. At ...


Expression Of Biologically Active Human Corticosteroid Binding Globulin By Insect Cells: Acquisition Of Function Requires Glycosylation And Transport, Jayasri Ghose-Dastidar, J. B. Alexander Ross, Reza Green Aug 1991

Expression Of Biologically Active Human Corticosteroid Binding Globulin By Insect Cells: Acquisition Of Function Requires Glycosylation And Transport, Jayasri Ghose-Dastidar, J. B. Alexander Ross, Reza Green

Chemistry and Biochemistry Faculty Publications

Human corticosteroid binding globulin (hCBG) is a 50- to 55-kDa serum glycoprotein that binds cortisol and progesterone with high affinity. To map the steroid-binding domain and to investigate the folding pathways of hCBG, we have established an expression system based on infection of insect cells with a recombinant baculovirus encoding hCBG. Infected Spodoptera frugiperda (Sf9) cells secrete immunoreactive hCBG at high levels (16-24 pmol per 106 cells per 40 h), and the recombinant protein binds cortisol with an affinity and specificity equivalent to that of human serum-derived hCBG. Thus, this system has the potential to provide large amounts of ...


Action Of Heptaminol Hydrochloride On Contractile Properties In Frog Isolated Twitch Muscle Fibre, Bruno Allard, Vincent Jacquemont, Fouad Lemtiri-Chlieh, Bernard Pourrias, Oger Rougier Jul 1991

Action Of Heptaminol Hydrochloride On Contractile Properties In Frog Isolated Twitch Muscle Fibre, Bruno Allard, Vincent Jacquemont, Fouad Lemtiri-Chlieh, Bernard Pourrias, Oger Rougier

fouad Lemtiri-Chlieh

1 Heptaminol stopped or delayed the progressive decline in tension which characterizes the phenomenon of fatigue in frog isolated twitch muscle fibre. 2 Heptaminol had no action on the sodium, potassium and calcium voltage-dependent ionic conductances. 3 The hypothesis of an action via an internal alkalinization was tested by comparison with the action of NH4Cl. Both substances increased the tension. 4 The action of hepataminol was suppressed in sodium-free (TRIS) solution or in the presence of amiloride while the action of NH4Cl was always observed. 5 These results could be explained by a stimulation of the Na/H antiport by ...


Er Translocation Intermediates Are Adjacent To A Nonglycosylated 34-Kd Integral Membrane Protein, Kennan V. Kellaris, Sharon Bowen, Reid Gilmore Jul 1991

Er Translocation Intermediates Are Adjacent To A Nonglycosylated 34-Kd Integral Membrane Protein, Kennan V. Kellaris, Sharon Bowen, Reid Gilmore

Open Access Articles

We have used the homobifunctional cross-linking reagent disuccinimidyl suberate (DSS) to identify proteins that are adjacent to nascent polypeptides undergoing translocations across mammalian rough ER. Translocation intermediates were assembled by supplementing cell free translations of truncated mRNAs with the signal recognition particle (SRP) and microsomal membrane vesicles. Two prominent cross-linked products of 45 and 64 kD were detected. The 64-kD product was obtained when the cell free translation contained SRP, while formation of the 45-kD product required both SRP and translocation competent microsomal membrane vesicles. In agreement with previous investigators, we suggest that the 64-kD product arises by cross-linking of ...


Heterogeneity Of Methotrexate Binding In Human Colon Tumor Cells, M P. Iqbal, N Mehboobali, M A. Waqar, M Ahmed Jun 1991

Heterogeneity Of Methotrexate Binding In Human Colon Tumor Cells, M P. Iqbal, N Mehboobali, M A. Waqar, M Ahmed

Department of Biological & Biomedical Sciences

[3H]-methotrexate binding at pH 5.0 and pH 7.2 by the cytosol of tumor tissues and the surrounding normal areas of the gastrointestinal tract of patients suffering from colon or gastric cancer has been used to identify in these cells the presence of a binder of methotrexate having low-affinity for this drug in addition to the enzyme dihydrofolate reductase. Scatchard analysis of the [3H]-methotrexate binding data by a colon tumor sample also reveals that there are two binders of this drug present in the cytosol of these cells. The association constant (Kass) for one binder of methotrexate ...


Cytolytic And Ion Channel Forming Properties Of The N-Terminus Of Lymphocyte Perforin, David M. Ojcius, Pedro M. Persechini, Li-Mou Zheng, Paulo C. Notaroberto, Sandro C. Adeodato, John Ding-E. Young Jun 1991

Cytolytic And Ion Channel Forming Properties Of The N-Terminus Of Lymphocyte Perforin, David M. Ojcius, Pedro M. Persechini, Li-Mou Zheng, Paulo C. Notaroberto, Sandro C. Adeodato, John Ding-E. Young

Dugoni School of Dentistry Faculty Articles

Perforin lyses cells by binding to the target cell membrane, where it polymerizes into large nonspecific pores. It is shown here that the first 34 amino acids of the N-terminal region of either human or murine perforin are soluble in aqueous medium and spontaneously insert into membranes. The N-terminal peptides lyse liposomes and nucleated cells, and they form ion channels in planar bilayers, some of which are comparable to those previously described for perforin. The lytic activity of the N-terminal domains does not require calcium, is independent of the lipid headgroup composition, and can be inhibited by heparin. Tumor cells ...


Mouse Mast Cell Proteases: Induction, Molecular Cloning, And Characterization, Wei Chu May 1991

Mouse Mast Cell Proteases: Induction, Molecular Cloning, And Characterization, Wei Chu

Electronic Theses and Dissertations

Tryptase, a mast cell-specific serine protease with trypsin-like specificity, has been identified in a mouse mast cell line (ABFTL-6) based on it's enzymatic activity, inhibition properties, and cross-reactivity to a human mast cell tryptase antibody. The effects of fibroblast-conditioned medium and sodium butyrate on ABFTL-6 mast cell differentiation and tryptase expression have been examined. ABFTL-6 mouse mast cells undergo phenotypic changes upon culturing in media supplemented with fibroblast-conditioned media at 50% or 1 mM sodium butyrate. The induced cells increased in size, had larger and more metachromatic cytoplasmic granules, and increased their total cellular protein about four-fold. Tryptase activity ...


Metabolism Of Arachidonate-Containing Phospholipid Molecular Species In The Murine Macrophage-Like Cell Line, P388d1, Crystal R. Waites May 1991

Metabolism Of Arachidonate-Containing Phospholipid Molecular Species In The Murine Macrophage-Like Cell Line, P388d1, Crystal R. Waites

Electronic Theses and Dissertations

Glycerophospholipids of mammalian cells exist as chemically diverse structures with various fatty acids at the sn-1 and sn-2 positions. Arachidonic acid, a polyunsaturated fatty acid, which may be converted to biologically active eicosanoids such as prostaglandins, thromboxanes, and leukotrienes, is found predominantly in the sn-2 position of glycerophospholipids. The purpose of this study was to examine, at the level of the individual molecular species, the incorporation of arachidonate into phospholipids and its release from phospholipids during stimulation. In this way, the specificity of the enzymes controlling arachidonate metabolism could be examined in order to clarify the processes that control the ...


Nitrogen Dioxide Reaction With Proteins: Evidence For Peptide Bond Cleavage At Lysine Residues, Darryl B. Hood May 1991

Nitrogen Dioxide Reaction With Proteins: Evidence For Peptide Bond Cleavage At Lysine Residues, Darryl B. Hood

Electronic Theses and Dissertations

Nitrogen dioxide (NO$\sb2$), an air pollutant produced by burning fossil fuels and a component of cigarette smoke, is thought to contribute to the pathogenesis of pulmonary diseases, such as emphysema. To gain information on the mechanism by which NO$\sb2$ damages the lung, in vitro exposures of $\alpha\sb1$-proteinase inhibitor ($\alpha\sb1$-PI), elastin, bovine serum albumin (BSA), human serum albumin (HSA) and synthetic poly-L-lysine were performed. A genetic deficiency of $\alpha\sb1$-PI predisposes humans to emphysema and NO$\sb2$ has been hypothesized to damage $\alpha\sb1$-PI, which would leave proteases such as human neutrophil elastase ...


Domains And Anomalous Adsorption Isotherms Of Dipalmitoylphosphatidylcholine Membranes And Lipophilic Ions: Pentachlorophenolate, Tetraphenylborate, And Dipicrylamine, Pavel Smejtek, S. Wang May 1991

Domains And Anomalous Adsorption Isotherms Of Dipalmitoylphosphatidylcholine Membranes And Lipophilic Ions: Pentachlorophenolate, Tetraphenylborate, And Dipicrylamine, Pavel Smejtek, S. Wang

Physics Faculty Publications and Presentations

Dipalmitoylphosphatidylcholine (DPPC) vesicles acquire negative surface charge on adsorption of negatively charged pentachlorophenolate (PCP-), and lipophilic ions tetraphenylborate (TPhB-), and dipicrylamine (DPA-). We have obtained (a) zeta-potential isotherms from the measurements of electrophoretic mobility of DPPC vesicles as a function of concentration of the adsorbing ions at different temperatures (25–42 degrees C), and (b) studied the effect of PCP- on gel-to-fluid phase transition by measuring the temperature dependence of zeta-potential at different PCP- concentrations. The zeta-potential isotherms of PCP- at 25, 32, and 34 degrees C correspond to adsorption to membrane in its gel phase. At 42 degrees C ...


Monovalent Cation Induced Structural Transitions In Telomeric Dnas: G-Dna Folding Intermediates, Charles C. Hardin, Eric Henderson, Thomas Watson, Joyce K. Prosser May 1991

Monovalent Cation Induced Structural Transitions In Telomeric Dnas: G-Dna Folding Intermediates, Charles C. Hardin, Eric Henderson, Thomas Watson, Joyce K. Prosser

Zoology and Genetics Publications

Telomeric DNA consists of G- and C-rich strands that are always polarized such that the G-rich strand extends past the 3’ end of the duplex to form a 12-16-base overhang. These overhanging strands can self-associate in vitro to form intramolecular structures that have several unusual physical properties and at least one common feature, the presence of non-Watson-Crick G-G base pairs. The term “G-DNA” was coined for this class of structures (Cech, 1988). On the basis of gel electrophoresis, imino proton NMR, and circular dichroism (CD) results, we find that changing the counterions from sodium to potassium (in 20 mM phosphate ...


Identification Of Laminin Domains That Promote Tumor Cell Adhesion, Corby Freitag Apr 1991

Identification Of Laminin Domains That Promote Tumor Cell Adhesion, Corby Freitag

Life and Environmental Sciences Undergraduate Theses

The process of metastasis requires tumor cells to migrate through the basement membrane which consists of laminin, a glycoprotein which promotes the adhesion of HT-1080 fibrosarcoma cells. The present study was designed to test four peptides, corresponding to short sequences of the intact laminin molecule, for cell adhesion-promoting activity. By using three different cell migration assays, the four peptides were monitored for their cell adhesive-promoting activity. Results were obtained showing some cell adhesive-promoting activity from all four peptides.


Cells That Express All Five Proteins Of Vesicular Stomatitis Virus From Cloned Cdnas Support Replication, Assembly, And Budding Of Defective Interfering Particles, Asit K. Pattnaik, Gail W. Wertz Feb 1991

Cells That Express All Five Proteins Of Vesicular Stomatitis Virus From Cloned Cdnas Support Replication, Assembly, And Budding Of Defective Interfering Particles, Asit K. Pattnaik, Gail W. Wertz

Papers in Veterinary and Biomedical Science

An alternative approach to structurefunction analysis of vesicular stomatitis virus (VSV) gene products and their interactions with one another during each phase of the viral life cycle is described. We showed previously by using the vaccinia viruslT7 RNA polymerase expression system that when cells expressing the nucleocapsid protein (N), the phosphoprotein (NS), and the large polymerase protein (L) of VSV were superinfected with defective interfering (DI) particles, rapid and efflicient replication and amplification of DI particle RNA occurred. Here, we demonstrate that all five VSV proteins can be expressed simultaneously when cells are cotransfected with plasmids containing the matrix protein ...


Thermodynamics Of Charged Oligopeptide Nucleic Acid Interactions, David Mascotti, T. Lohman Jan 1991

Thermodynamics Of Charged Oligopeptide Nucleic Acid Interactions, David Mascotti, T. Lohman

David P. Mascotti

No abstract provided.


Interleukin-1 Or Phorbol Induction Of The Stromelysin Promoter Requires An Element That Cooperates With Ap-1., Karen Sirum-Connolly, Constance E. Brinckerhoff Jan 1991

Interleukin-1 Or Phorbol Induction Of The Stromelysin Promoter Requires An Element That Cooperates With Ap-1., Karen Sirum-Connolly, Constance E. Brinckerhoff

Open Dartmouth: Faculty Open Access Articles

Interieukin-1, a mediator of inflammation, or tumor promoting phorbol esters induce transcription of stromelysin, a metalloproteinase that degrades extracellular matrix molecules and that is overexpressed in diseases such as rheumatoid arthritis. Sequences required for induction of transcription of the human stromelysin promoter are contained on a 46 base pair fragment. This fragment contains a sequence with a high degree of similarity to the binding site for the transcription factor activator protein-1 (AP-1) and indeed, the AP-1 sequence of this fragment is necessary but not sufficient for the maximal response to phorbol 12-myristate 13-acetate (phorbol) or interleukin-1. Maximal induction requires functional ...


Use Of Affinity Chromatography In Developing Acridinium Ester-Labeled Antibodies For An Immunometric Assay Of Parathyrin, David S. Hage, Bob Taylor, Pat Schryver, Pai C. Kao Jan 1991

Use Of Affinity Chromatography In Developing Acridinium Ester-Labeled Antibodies For An Immunometric Assay Of Parathyrin, David S. Hage, Bob Taylor, Pat Schryver, Pai C. Kao

David Hage Publications

In developing an immunometric assay of intact parathyrin (parathyroid hormone, PTH), we found that affinity chromatography is a useful tool in purifying and optimizing the labeling conditions for acridinium ester-labeled antibodies. ... In summary, affinity chromatography was found to be useful in the purification of acridinium ester-labeled antibodies, particularly for removing denatured antibodies from the prepared label and for monitoring the amount of active labeled antibodies produced.


Purification And Properties Of The Malic Enzyme And The Malate Dehydrogenases From The Gill Tissue Of The Ribbed Mussel (Modiolus Demissus) , Mary Michael Brodey Jan 1991

Purification And Properties Of The Malic Enzyme And The Malate Dehydrogenases From The Gill Tissue Of The Ribbed Mussel (Modiolus Demissus) , Mary Michael Brodey

Retrospective Theses and Dissertations

To assess the role of dicarboxylic acid metabolism in the synthesis of alanine and other compounds by the ribbed mussel under conditions of hyperosmotic and anaerobic stress, the cytosolic and mitochondrial malate dehydrogenases and the mitochondrial malic enzyme from this organism were purified and their kinetics studied. Both the cytosolic and mitochondrial malate dehydrogenases were found to be dimers with native M[subscript] r = 60,000. One cMDH allele (designated slow) was found to predominate in populations from Cape Cod, MA. Similar low K[subscript] m's were observed for purified preparations of the slow/slow homodimer (cMDH) and for ...


Selective Labeling Of Peptides, Metalloproteins, And Proteolytic Enzymes With Pt(Ii) Complexes And Electron-Transfer Reactions Of Native And Cross-Linked Metalloproteins , Herb M. Brothers Ii Jan 1991

Selective Labeling Of Peptides, Metalloproteins, And Proteolytic Enzymes With Pt(Ii) Complexes And Electron-Transfer Reactions Of Native And Cross-Linked Metalloproteins , Herb M. Brothers Ii

Retrospective Theses and Dissertations

The transition-metal complex (Pt(trpy)Cl) [superscript]+ selectively labels the amino acids Cys and His, but is unreactive toward Met. The (Pt(trpy)Cl) [superscript]+ complex also forms complexes with nonbiological ligands to form novel compounds with metal-metal or [pi]-[pi] interactions;The homologous gold reagent, (Au(trpy)Cl) [superscript]2+ reacts with Trp, Cys, His, and Met. The oxidative properties of the gold complex makes it an invasive reagent toward proteins, however;In reactions with proteins, the complex (Pt(trpy)Cl) [superscript]+ exhibits unexpected selectivity toward amino acid side chains in cytochromes c. His residues are labeled in greater ...


In Vitro Desmin Assembly And In Vivo Changes In Developing Smooth Muscle , Rong-Ghi Robert Chou Jan 1991

In Vitro Desmin Assembly And In Vivo Changes In Developing Smooth Muscle , Rong-Ghi Robert Chou

Retrospective Theses and Dissertations

This dissertation is divided into two principal parts. The purpose of the first part was to investigate the effects of cations and temperature on the critical concentration of desmin required for filament assembly by using tow independent assays, namely, turbidity and centrifugation. Assembly was done in the presence of 2 mM Ca[superscript]2+, 2 mM Mg[superscript]2+ or 150 mM Na[superscript]+ at 2, 22 and 37°C. Similar values for critical concentration were obtained by both assays. As temperature increased, critical concentration decreased for each cation. Critical concentration was lowest in the presence of Ca[superscript]2 ...


Changes In Gene Expression During Induction Of The Carbon Dioxide Concentrating Mechanism In Chlamydomonas Reinhardtii , Thomas Leslie Winder Jan 1991

Changes In Gene Expression During Induction Of The Carbon Dioxide Concentrating Mechanism In Chlamydomonas Reinhardtii , Thomas Leslie Winder

Retrospective Theses and Dissertations

The regulation of genes by CO[subscript]2 concentration in wild type and mutants of the unicellular green alga Chlamydomonas reinhardtii was studied. This and other algal species can photosynthesize efficiently under limiting concentrations of CO[subscript]2 due to the activities of a CO[subscript]2 concentrating mechanism and carbonic anhydrase. A cDNA copy of the gene that codes for a periplasmic carbonic anhydrase (perCA) was isolated and used to study regulation of perCA synthesis in a cell wall-deficient mutant (CW-15). Over-expression of perCA in CW-15, relative to wild type, was found at the levels of enzyme activity, protein ...


Molecular Mechanisms Controlling Bovine Lymphocyte Homing , Brad Thomas Bosworth Jan 1991

Molecular Mechanisms Controlling Bovine Lymphocyte Homing , Brad Thomas Bosworth

Retrospective Theses and Dissertations

The cDNA clones encoding for the bovine glycoproteins CD44 and LECAM-1 (lectin cell adhesion molecule-1) were isolated and sequenced. The predicted amino acid sequences of bovine CD44 and LECAM-1 had high identities with those of human and murine CD44 and LECAM-1. The high identities of the predicted amino acid sequences of these glycoproteins in the bovine, human and mouse suggest they may have similar functions in all three species. While the overall amino acid identities were high, bovine CD44 had an area of low identity with human and murine CD44 in the extracellular domain and the cytoplasmic tail of bovine ...


Nicotinate, Nicotinamide, And The Reactivity Of Leghemoglobin In Soybean Root Nodules, Robert V. Klucas, Cyril A. Appleby Jan 1991

Nicotinate, Nicotinamide, And The Reactivity Of Leghemoglobin In Soybean Root Nodules, Robert V. Klucas, Cyril A. Appleby

Biochemistry -- Faculty Publications

Nicotinate has been postulated to interfere with the binding of O2 to ferrous leghemoglobin in soybean (Glycine max) root nodules. For such a function, the levels of nicotinate in nodules must be sufficiently high to bind a significant amount of leghemoglobin. We have measured levels of nicotinate, nicotinamide, and leghemoglobin in soybean nodules from plants 34 to 73 days after planting in a glasshouse. On a per gram nodule fresh weight basis, levels between 10.4 and 21 nanomoles for nicotinate, 19.2 and 37.8 nanomoles for nicotinamide, and 170 to 280 nanomoles for leghemoglobin were measured. Even ...