Open Access. Powered by Scholars. Published by Universities.®

Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

1984

Discipline
Institution
Keyword
Publication
Publication Type

Articles 1 - 26 of 26

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Characterization Of Kpni Interspersed, Repetitive Dna Sequence Families And Their Association With The Nuclear Matrix, Joseph A. Chimera Dec 1984

Characterization Of Kpni Interspersed, Repetitive Dna Sequence Families And Their Association With The Nuclear Matrix, Joseph A. Chimera

Electronic Theses and Dissertations

The KpnI, 1.2 and 1.5 kb families of interspersed repetitive DNAs from the African green monkey genome were isolated and characterized. Each family contains three populations of segments based on their sequence lengths and susceptibility to cleavage by the restriction enzymes KpnI and RsaI. The first population contains the smallest segments which are susceptible to both KpnI and RsaI cleavage and have fragment lengths of 1.2 kb (1.2 kb family) and 1.5 kb (1.5 kb family) respectively. The members in this population are referred to as KpnI-sensitive segments. The second population contains longer segments ...


Characterization Of The Transport Of Oxaloacetate By Pea Leaf Mitochondria, David J. Oliver, Griffin H. Walker Oct 1984

Characterization Of The Transport Of Oxaloacetate By Pea Leaf Mitochondria, David J. Oliver, Griffin H. Walker

David J. Oliver

Mitochondria isolated from pea (Pisum sativum L.) leaves are able to transport the keto acid, oxaloacetate, from the reaction medium into he mitochondrial matrix at high rates. The rate of uptake by the mitochondria was measured as the rate of disappearance of oxaloacetate from the reaction medium as it was reduced by matrix malate dehydrogenase using NADH provided by glycine oxidation. The oxaloacetate transporter was identifed as being distinct from the dicarboxylate and the α-ketoglutarate transporters because of its inhibitor sensitivities and its inability to interact with other potential substrates. Phthalonate and phthalate were competitive inhibitors of oxaloacetate transport with ...


Phorbol Ester Induces Desensitization Of Adenylate Cyclase And Phosphorylation Of The Beta-Adrenergic Receptor In Turkey Erythrocytes, Daniel J. Kelleher, Jeffrey E. Pessin, Arnold E. Ruoho, Gary L. Johnson Jul 1984

Phorbol Ester Induces Desensitization Of Adenylate Cyclase And Phosphorylation Of The Beta-Adrenergic Receptor In Turkey Erythrocytes, Daniel J. Kelleher, Jeffrey E. Pessin, Arnold E. Ruoho, Gary L. Johnson

Biochemistry and Molecular Pharmacology Publications and Presentations

Incubation of turkey erythrocytes with the phorbol ester phorbol 12-myristate 13-acetate (PMA) results in a dose- and time-dependent desensitization of isoproterenol-stimulated adenylate cyclase activity. Compared to controls, membranes from PMA-treated cells have an isoproterenol-stimulated adenylate cyclase activity that is decreased 20%-40%, with little effect on forskolin or fluoride activation of adenylate cyclase. No change in beta-adrenergic receptor number is observed after PMA treatment, indicating that the major effect of PMA is to uncouple receptor interactions with Ns, the stimulatory guanine nucleotide regulatory protein of adenylate cyclase. Purification of beta-adrenergic receptors from 32Pi-labeled turkey erythrocytes, incubated in the presence or ...


Double-Stranded Ribonucleic Acid Killer Systems In Yeasts, Donald J. Tipper, Kieth A. Bostian Jun 1984

Double-Stranded Ribonucleic Acid Killer Systems In Yeasts, Donald J. Tipper, Kieth A. Bostian

Microbiology and Physiological Systems Publications and Presentations

Killer yeasts secrete polypeptide toxins which kill sensitive cells of their own species and frequently those of other species and genera of yeast. In this review we will focus on the molecular biology of the most intensively investigated killer system, the M1- dsRNA-determined Kl killer system of S. cerevisiae. We hope to demonstrate the relevance of this system to many of the vital issues in yeast and general eucaryotic molecular biology.


Time Course Of Enzyme Catalyzed Reactions: The Stoichiometry A --> P + Q, Tamra Tuttle May 1984

Time Course Of Enzyme Catalyzed Reactions: The Stoichiometry A --> P + Q, Tamra Tuttle

All Graduate Theses and Dissertations

The feasibility of using an integrated rate equation to analyze the kinetics of a second-order, enzyme-catalyzed reaction has been investigated. The inducible arginine decarboxylase from Escherichia coli B. was chosen for this study because it catalyzes an irreversible reaction with stoichiometry A --> P + Q, the simplest second order case. Values for five of the eight possible kinetic constants were determined from 21 time courses. Of the remaining three, the uncompetitive product inhibition constant for CO2 was shown to be between O and 0.06, while the values of the competitive product inhibition for CO2 and the uncompetitive constant for arginine ...


Photoaffinity Labeling Of The Antimycin Binding Site In Rhodopseudomonas Sphaeroides, Emily Wilson May 1984

Photoaffinity Labeling Of The Antimycin Binding Site In Rhodopseudomonas Sphaeroides, Emily Wilson

All Graduate Theses and Dissertations

The purpose of this study was to identify the site of interaction of antimycin with the ubiquinone-cytochrome b-c1 oxidoreductase in the photosynthetic bacteria, Rhodopseudomonas sphaeroides. To accomplish this goal, three areas of research were undertaken: the synthesis of a radiolabeled, photoaffinity analog of antimycin, identification of the inhibitory characteristics of this analog, and the photoaffinity labeling of the antimycin binding site. All three areas were accomplished.

The major finding of this study was the identification of an 11,000 dalton polypeptide as the predominantly labeled protein. Although this polypeptide was not exclusively labeled, it was consistently labeled and showed competition ...


Purification And Characterization Of A Camp- And Ca2+-Calmodulin-Independent Glycogen Synthase Kinase From Porcine Renal Cortex, Stephen J. Beebe, Erwin M. Reimann, Keith K. Schlender Feb 1984

Purification And Characterization Of A Camp- And Ca2+-Calmodulin-Independent Glycogen Synthase Kinase From Porcine Renal Cortex, Stephen J. Beebe, Erwin M. Reimann, Keith K. Schlender

Bioelectrics Publications

We recently reported the partial purification of a cAMP-independent and Ca2+-calmodulin-independent glycogen synthase kinase from porcine renal cortex (Schlender, K. K., Beebe, S. J., and Reimann, E. M. (1981) Cold Spring Harbor Conf. Cell Proliferation, 389-400). Subsequent purification indicated that the enzyme preparation consisted of at least three forms of glycogen synthase kinase which could be resolved by ATP gradient elution from aminoethylphosphate-agarose (AEP-agarose). The predominant form of glycogen synthase kinase, which eluted from AEP-agarose between 2 and 6 mM ATP, was purified approximately 800-fold and is designated GSK-A1. It had a molecular weight of 45,000-50,000 ...


Some Plant Leaves Have Orientation-Dependent Epr And Nmr Spectra, Douglas C. Mccain, Ted C. Selig, Govindjee, John L. Markley Feb 1984

Some Plant Leaves Have Orientation-Dependent Epr And Nmr Spectra, Douglas C. Mccain, Ted C. Selig, Govindjee, John L. Markley

Faculty Publications

Proton nuclear magnetic resonance (1H NMR) spectra of leaves from 50 plant species were obtained at a spectrometer frequency of 470 MHz. Water present in leaf samples gives rise to characteristic spectral patterns. Most species show only one broad 1H NMR peak; however, the leaves of some plants display complex, orientation-dependent spectra in which a common three-line pattern is discerned. The pattern varies with the angle between the leaf surface and the external magnetic field. Proton relaxation measurements show the presence of at least two water compartments in the leaves. The compartments are responsible for different components of ...


Extracellular Phosphodiesterase From The Growth Medium Of The Myxomycete Physarum Flavicomum, Thomas J. Lynch, Mary E. Farrell Jan 1984

Extracellular Phosphodiesterase From The Growth Medium Of The Myxomycete Physarum Flavicomum, Thomas J. Lynch, Mary E. Farrell

Journal of the Arkansas Academy of Science

The plasmodium of the myxomycete Physarum flavicomum secretes cyclic AMP phosphodiesterase into the medium. The extracellular enzyme had a pH optimum between 7 and 8 and a Km of about 500 μM cyclic AMP and was inhibited by theophylline, caffeine and 3-isobutyl-l-methyxanthine (MIX). A marked decrease of enzyme activity was noted in the presence of EDTA, suggesting the requirement of Mg+ + by the enzyme. Addition of Mg+ + and Ca + + stimulated the enzyme while Zn+ + , Co+ + , Pb+ + , Mn+ + , Fe + + + , Ni+ + , and Cu + + all inhibited phosphodiesterase activity. An interesting feature of this extracellular phosphodiesterase was its ability to retain full catalytic activity ...


Cerebral Glutamine/Glutamate Interrelationships And Metabolic Compartmentation / S. Berl And D. D. Clarke, Soll Berl, Donald Dudley Clarke Phd Jan 1984

Cerebral Glutamine/Glutamate Interrelationships And Metabolic Compartmentation / S. Berl And D. D. Clarke, Soll Berl, Donald Dudley Clarke Phd

Chemistry Faculty Publications

The fact that in animals the concentration of glutamate and glutamine are higher in brain than in any other tissue initially attracted interest in attempting to understand the function or functions of these amino acids in the central nervous system. They were evidently required for protein synthesis and early studies established their close relationship to the Krebs tricarboxylic acid cycle and energy metabolism in brain. Glutamine formation in brain was also shown to be sensitive to elevations in plasma ammonia levels and to serve as the primary method for detoxifying this substance in brain in contrast to urea formation in ...


Potassium 1,3,3a,8a-Tetrahydro-3a,8a-Dihydroxy-2,8-Dioxoindeno[1,2-D]Imidazole-2-Ethanesulfonate, K+.C12h11n2o7s- / By D. D. Bray And D. D. Clarke Chemistry Department, Fordham University, Bronx, Nyew York 10458, Usa And N. Chatterjie And B. Sinha Institute For Basic Research In Development Disabilities, 1050 Forest Hill Road, Staten Island, New York 10314, Usa, Diana Bray, Donald Dudley Clarke Phd, Nithiananda Chatterjie, B. Sinha Jan 1984

Potassium 1,3,3a,8a-Tetrahydro-3a,8a-Dihydroxy-2,8-Dioxoindeno[1,2-D]Imidazole-2-Ethanesulfonate, K+.C12h11n2o7s- / By D. D. Bray And D. D. Clarke Chemistry Department, Fordham University, Bronx, Nyew York 10458, Usa And N. Chatterjie And B. Sinha Institute For Basic Research In Development Disabilities, 1050 Forest Hill Road, Staten Island, New York 10314, Usa, Diana Bray, Donald Dudley Clarke Phd, Nithiananda Chatterjie, B. Sinha

Chemistry Faculty Publications

Mr = 366·40, monoclinic, P2/c, Z = 4, a=6·195 (1), b= 17·701 (4), c= 1.2·864 (3)A, P= 116·25(2)0 , V=l387·8A3, Dm=1·74, Dx= 1·754 Mg m-3, CuKa, l = 1·5418 A, ,u = 5·07 mm-1, T= 298 K, F(OOO) = 752, R = 0·074 for 2091 observed independent intensity data. Crystals were prepared by reacting the K salt of ureidotaurine with ninhydrin in acid solution. The product shows that the unsubstituted N -of the ureidotaurine reacts with the central C of ninhydrin


Las Vegas Wash Advanced Water Quality Study: Final Report, Richard A. Roline, James J. Sartoris, U.S. Bureau Of Reclamation Jan 1984

Las Vegas Wash Advanced Water Quality Study: Final Report, Richard A. Roline, James J. Sartoris, U.S. Bureau Of Reclamation

Publications (WR)

The purpose of the Las Vegas Wash Advanced Water Quality Study is to determine the existence, extent, and mechanisms of nutrient and toxin stripping in Las Vegas Wash under present conditions and under future conditions, both with and without construction of the proposed salinity control unit as described by the Bureau of Reclamation (USBR, 1982b). This study was performed for the Lower Colorado Region Division of Planning by personnel of the Environmental Sciences Section of the Division of Research and Laboratory Services, E&R Center, Denver, Colorado. Work on the study began in February 1983.

The general approach adopted for this study consisted of three parts:

1. Four regular field samplings (in February, June, July, and August 1983) and one storm event sampling (in March 1983) to assess present water quality conditions in the wash. Additional water samples were collected in September to identify changes, if any, in water quality following a major storm event.

2. A thorough review of all available data from previous studies of Las Vegas Wash to assess water quality changes and trends over the past 10 to 15 years.

3. A review of the technical literature on wetlands as water treatment systems to provide insight into the possible physical, chemical, and biological mechanisms operating in the marsh and to serve as a theoretical basis for extrapolating various future conditions.

Thirteen water quality sampling stations were established in and around Las Vegas Wash for the assessment of present conditions. They included the same stations used in the WQSS (Water Quality Standards Study) performed by Brown and Caldwell 1/ in 1979-81 (stas. 1 through 5). Three of these stations are located at USGS (U.S. Geological Survey) stream gaging stations (stas. 1, 3, and 5), and two of them (stas. 1 and 5) are also USGS water qua!ity monitoring stations.

Five stations are located on various "tributaries" of Las Vegas Wash and represent a range of water quality inputs to the wash and, in some cases, to the proposed bypass channel. These stations are located as follows:

1. MRF (Monson Road Floodway)

2. TAF (Tropicana Avenue Floodway)

3. DC (Duck Creek)

4. AD (Alpha Ditch)

5. GWD (the ground-water drain which crosses Pabco Road just south of the wash)

Finally, three additional stations (stas. 2A, 2B, and 3A) were established as the study progressed in an effort to better define changes between channelized and marsh sections of the wash…

This report is divided into two separate sections, dealing with toxics and nutrients, respectively. Field measurements and sampling for both aspects of this study were carried out as a single operation on the dates listed in table 1; however, data analyses and interpretation were performed separately. The final section of this report draws together and ...


Studies On The Interactions Of Low Molecular Weight Ribosomal Rnas And Ribosomal Proteins , Janice Ann Kolberg Jan 1984

Studies On The Interactions Of Low Molecular Weight Ribosomal Rnas And Ribosomal Proteins , Janice Ann Kolberg

Retrospective Theses and Dissertations

For an understanding of the molecular events that occur on the ribosome during protein biosynthesis, the interaction of proteins and RNA within the ribosome must be understood. In this study, the interaction of ribosomal RNAs with ribosomal proteins was studied using an affinity chromatography procedure. Immobilized RNA columns were prepared by first oxidizing the 3' terminus of the RNA and then covalently coupling this to dihydrazide-Sepharose. In some experiments, columns were prepared in which RNA was attached to dihydrazide-Sepharose through its 5' terminus. Initial experiments investigated the interaction of 3'-bound E. coli 5S RNA with E.coli 50S ribosomal ...


Synthesis And Characterization Of Some Oxoperoxopolycarboxylato Vanadates (V) And Their Biochemical Significance, Myunghi Lee Jan 1984

Synthesis And Characterization Of Some Oxoperoxopolycarboxylato Vanadates (V) And Their Biochemical Significance, Myunghi Lee

Dissertations, Theses, and Masters Projects

No abstract provided.


The Effect Of Nucleosides On The Expression Of The Glycoprotein Hormone Alpha Subunit And Placental Alkaline Phosphatase In Hela Cells , Richard Neil Tamura Jan 1984

The Effect Of Nucleosides On The Expression Of The Glycoprotein Hormone Alpha Subunit And Placental Alkaline Phosphatase In Hela Cells , Richard Neil Tamura

Retrospective Theses and Dissertations

The production of the human chorionic gonadotropin alpha subunit and placental alkaline phosphatase (PAP) activity varied among different HeLa cell lines with regard to both constitutive and butyrate (Btr) induced expression. No correlation was observed between the extent of Btr induction and the constitutive level of synthesis for a given cell line. Moreover, Bts did not produce similar increases of both PAP and alpha, suggesting that the expression of these two genes is not coordinately regulated. Analysis of the subunits by column chromatography suggests that the difference in production rates for the cell lines is not due to differences in ...


Porcine Pancreatic Α-Amylase Hydrolysis Of Modified Substrates And The Specificity Of Subsite Binding , Paul J. Braun Jan 1984

Porcine Pancreatic Α-Amylase Hydrolysis Of Modified Substrates And The Specificity Of Subsite Binding , Paul J. Braun

Retrospective Theses and Dissertations

The effect of substrate modification on the action of PPA has been determined for five (alpha)-(1(--->)4)-glucans in which a small proportion of the glucose residues (15% or less) contained modified functional groups. Chemical and enzymatic techniques were used to synthesize four substrates (6-deoxyamylose, mono-6-deoxy-6-fluoro-(beta)-cyclodextrin, mono-2-deoxy-(beta)-cyclodextrin, and amylose containing D-allose residues) in which hydrogen bond-forming capabilities were altered or removed. Hydrolysis of these substrates by PPA produced two isomers of modified disaccharides and modified monosaccharide. These results indicate that modifications of this type may be productively bound at subsites 1 through 4 of the ...


Synthesis Of Messenger-Like Rna By Isolated Avian Erythrocyte Nuclei , Paul Allen Wiersma Jan 1984

Synthesis Of Messenger-Like Rna By Isolated Avian Erythrocyte Nuclei , Paul Allen Wiersma

Retrospective Theses and Dissertations

Cell ghosts have been prepared from mature chicken erythrocytes using 0.05% saponin. Such preparations provide a system, where the nucleus is permeable to nucleotides and macromolecules, for studying the low level RNA synthesis characteristic of these cells. Although several fold lower than that in HeLa cell nuclei, the ('3)HUTP incorporation in the erythrocyte can be detected using high specific activity isotope and assaying on DEAE filters with extensive washing to reduce background. RNase A (50 (mu)g/ml) and KOH (0.3M) eliminate all radioactivity binding to filters, indicating the product is RNA. Preincubation with DNase I (10 ...


19f Nmr Studies Of The Solution Structure And Dynamics Of 5-Fluorouracil-Substituted Valine Trna From Escherichia Coli , Charles Clifford Hardin Jan 1984

19f Nmr Studies Of The Solution Structure And Dynamics Of 5-Fluorouracil-Substituted Valine Trna From Escherichia Coli , Charles Clifford Hardin

Retrospective Theses and Dissertations

Valine tRNA was purified from 5-fluorouracil (FUra) treated E. coli cells and resolved into two isoaccepting species, termed FUra tRNA(,1)('Val) forms (A) and (B), respectively. The ('19)F NMR spectrum of the (B) form contains 13 resolved resonances for the 14 incorporated FUra residues dispersed from 1.6 and 8.5 ppm (FUra = 0 ppm). ('19)F NMR spectra of the (A) and (B) forms differ in the shift of peak E from ca. 4.3 ppm in form (B) upfield to ca. -15 ppm in form (A). In light of previous sequence studies with FUra tRNA(,F ...


[Alpha]-Amylase Action And 13c-Nmr Studies On Amylose-V Complexes And Retrograded Amylose, Jay-Lin Jane Jan 1984

[Alpha]-Amylase Action And 13c-Nmr Studies On Amylose-V Complexes And Retrograded Amylose, Jay-Lin Jane

Retrospective Theses and Dissertations

Aqueous amylodextrins form helical complexes with alcohol, halogenated hydrocarbons, and triiodide known as V-complexes. With the formation of these complexes, the ('13)C-nuclear magnetic resonance (n.m.r.) signals of carbons 1 and 4 are shifted substantially downfield due to rotations about the glycosidic bond necessary to form the helical conformation. ('13)C-N.m.r. studies with amylodextrin, (alpha)-methyl D-glucoside, and (alpha)-methyl maltoside complexes suggest a complexing mechanism in which pyranose ring strain, induced by binding of the complexing agent, is relieved by rotations about the glycosidic bonds.;Retrograded amylodextrin, gives ('1)H and ('13)C n.m ...


The Marine Biogeochemistry Of Selenium: A Re-Evaluation, Gregory A. Cutter, Kenneth W. Bruland Jan 1984

The Marine Biogeochemistry Of Selenium: A Re-Evaluation, Gregory A. Cutter, Kenneth W. Bruland

OEAS Faculty Publications

Vertical and horizontal profiles from the North and South Pacific Oceans demonstrate the existence of three species of dissolved selenium: selenite, selenate, and organic selenide (operationally defined). In surface waters, organic selenide makes up about 80% of the total dissolved selenium, selenite concentrations are uniformly low, and selenate concentrations rise with increased vertical mixing. The organic selenide maximum (thought to consist of seleno-amino acids in peptides) coincides with the maxima of primary productivity, pigments, bioluminescence, and dissolved free amino acids. Deep ocean waters are enriched in selenite and selenate, while organic selenide is nondetectable. In suboxic waters of the tropical ...


Breed And Swine Lymphocyte Antigen Haplotype Differences In Agglutination Titers Following Vaccination With B. Bronchiseptica, Max F. Rothschild, H. L. Chen, L. L. Christian, W. R. Lie, L. Venier, M. Cooper, C. Briggs, C. M. Warner Jan 1984

Breed And Swine Lymphocyte Antigen Haplotype Differences In Agglutination Titers Following Vaccination With B. Bronchiseptica, Max F. Rothschild, H. L. Chen, L. L. Christian, W. R. Lie, L. Venier, M. Cooper, C. Briggs, C. M. Warner

Animal Science Publications

Genetic differences in immune response to B. bronchiseptica after vaccination with a commercial B. bronchiseptica bacterin were investigated in 1,069 8-wk-old pigs. These pigs were from 65 litters born in the spring and 66 litters born in the fall of 1982 and were purebreds from the Chester White (n = 128), Duroc (n = 281), Hampshire (n = 143), Landrace (n = 309) and Yorkshire (n = 208) breeds. Each litter was raised separately. Individual pigs were vaccinated im at 4 and 6 wk of age with 2 ml of B. bronchiseptica bacterin. At 8 wk of age, 8 ml of blood were collected ...


Reduction Of Ferric Leghemoglobin In Soybean Root Nodules, Keuk-Ki Lee, Robert V. Klucas Jan 1984

Reduction Of Ferric Leghemoglobin In Soybean Root Nodules, Keuk-Ki Lee, Robert V. Klucas

Biochemistry -- Faculty Publications

Reduction of ferric leghemoglobin to ferrous leghemoglobin in soybean nodules (Glycine max [L.] Meff. cv Woodworth) was studied using a spectrophotometer equipped with an in-cell space diffuse reflectance accessory. Nodule slices prepared and scanned under nitrogen gas showed a ferrous leghemoglobin absorption spectrum. Nodule slices equilibrated with 100% O2 or air exhibited two absorption bands characteristic of oxygenated leghemoglobin. The addition of CO shifted those bands to CO leghemoglobin absorption bands. Potassium ferricyanide was not effective in oxidizing ferrous to feffic leghemoglobin in nodule slices. However, ferric leghemoglobin was formed by treating the nodule slices with hydroxylamine, and this ...


Soybean Isoflavones: Estrogenic Activity And Isolation Of Two New Isoflavones , Efi D. Farmakalidis Jan 1984

Soybean Isoflavones: Estrogenic Activity And Isolation Of Two New Isoflavones , Efi D. Farmakalidis

Retrospective Theses and Dissertations

A rapid method utilizing preparative high-performance liquid chromatography was developed for the isolation and purification of the soybean isoflavones genistin and daidzin;Significant differences in the estrogenic response of different mouse strains to diethylstilboestrol (DES) and the phytoestrogens were observed. The inbred strains B6D2F1 and B6C3F1 were significantly more sensitive to DES than the outbred ICR and CD-1 mice. The inbred mouse strain B6D2F1 was used to investigate the estrogenic potency of genistin and diadzin. The estrogenic response to 1.5 mg of genistin was equivalent to 1 mg of genistein, giving a 1:1 molar relationship in estrogenic potency ...


Microheterogeneity Of Type Ii Camp-Dependent Protein Kinase In Various Mammalian Species And Tissues, Alison M. Robinson-Steiner, Stephen J. Beebe, Stephen R. Rannels, Jackie D. Corbin Jan 1984

Microheterogeneity Of Type Ii Camp-Dependent Protein Kinase In Various Mammalian Species And Tissues, Alison M. Robinson-Steiner, Stephen J. Beebe, Stephen R. Rannels, Jackie D. Corbin

Bioelectrics Publications

Excluding autophosphorylated species, at least six forms of the regulatory subunit of type II cAMP-dependent protein kinase (R(II)) from various mammalian tissues were identified by sodium dodecyl sulfate (SDS) gel electrophoresis of purified samples and of crude preparations photoaffinity labeled with 8-azido[32P] cAMP and by gel filtration. After autophosphorylation some heart R(II) forms termed type IIA (bovine, porcine, equine, and dog) shifted to a more slowly migrating band on SDS gels while others termed type IIB (rat, guinea pig, rabbit, and monkey) did not detectably shift. Both subclasses of R(II) exhibited variation in apparent M(r ...


Two Classes Of Camp Analogs Which Are Selective For The Two Different Camp-Binding Sites Of Type Ii Protein Kinase Demonstrate Synergism When Added Together To Intact Adipocytes, Stephen J. Beebe, Rob Holloway, Stephen R. Rannels, Jackie D. Corbin Jan 1984

Two Classes Of Camp Analogs Which Are Selective For The Two Different Camp-Binding Sites Of Type Ii Protein Kinase Demonstrate Synergism When Added Together To Intact Adipocytes, Stephen J. Beebe, Rob Holloway, Stephen R. Rannels, Jackie D. Corbin

Bioelectrics Publications

Twenty-five cyclic nucleotide analogs were tested individually to act as lipolytic agents and to activate adipocyte protein kinase. The lipolytic potency of individual analogs correlated better with their K(a) for protein kinase and their lipophilicity rather than with either parameters alone. Some of the most potent lipolytic analogs had high I50 values for the particulate low K(m) cAMP phosphodiesterase suggesting that their effect was not due to raising endogenous cAMP levels through inhibition of phosphodiesterase. The most potent lipolytic analogs contained a thio moiety at the C-8 or C-6 position. These analogs exhibited concave upward dose-response curves. At ...


Comparison Of Surface Polysaccharides Of R. Leguminosarum Anu57 (Exo-1), R. Leg. Anu57(Pbr1an) (Nod+, Nif-), And R. Leg. Anu57(Pjb5j1) (Nod+, Nif-), Frank G. Hustmyer Jan 1984

Comparison Of Surface Polysaccharides Of R. Leguminosarum Anu57 (Exo-1), R. Leg. Anu57(Pbr1an) (Nod+, Nif-), And R. Leg. Anu57(Pjb5j1) (Nod+, Nif-), Frank G. Hustmyer

Masters Theses

Extracellular polysaccharides (EPS) and lipopolysaccharides (LPS) are suspected to be involved in the legume/Rhizobium symbiotic process. Polysaccharide compositions from the EPS, LPS, capsular polysaccharide (CPS), and small polysaccharide (SmPS) were compared between Rhizobium leguminosarum ANU57 (parent, Exo-1) and two mutants R. leg. ANU57(pBR1AN) (nod+, nif-) and R. leg. ANU57(pJB5J1) (nod+, nif-) both containing a Sym (symbiotic) plasmid insertion. R. leg. ANU57(pBR1AN) and R. leg. ANU57(pJB5J1) are nod+ on clover and peas, respectively. The nod+ mutants produce larger amounts of EPS and decreased amounts of LPS compared to the parental strain. However, the EPS found ...