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Retrospective Theses and Dissertations

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Articles 31 - 60 of 776

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Characterization Of Three Spindle Matrix Proteins, Megator, East And Asator, In Drosophila, Hongying Qi Jan 2007

Characterization Of Three Spindle Matrix Proteins, Megator, East And Asator, In Drosophila, Hongying Qi

Retrospective Theses and Dissertations

Two previously identified proteins Skeletor and Chromator provide the molecular evidence for the existence of a spindle matrix complex in Drosophila. The spindle matrix has been proposed to be a macromolecular complex, which plays a role in organization and stabilization of the mitotic spindle as well as in providing structural support for counterbalancing force production.;In this dissertation, I present the identification and characterization of three more putative spindle matrix candidate proteins, Megator, EAST and Asator. Megator, an ortholog of the mammalian TPR protein, contains a large coiled-coil domain at its NH2-terminus and an acidic, non-structural COOH-terminus. Immunohistochemistry studies show ...


Mechanism Of Cap-Independent Translation By Long Distance Kissing Stem-Loop Interactions In Plant Viral Rnas, Aurélie Mamisoa Rakotondrafara Jan 2007

Mechanism Of Cap-Independent Translation By Long Distance Kissing Stem-Loop Interactions In Plant Viral Rnas, Aurélie Mamisoa Rakotondrafara

Retrospective Theses and Dissertations

While the presence of a 5' cap and a 3' poly(A) tail are key elements for efficient translation of eukaryotic mRNAs, many viral mRNAs lack one or both of such structures and yet translate efficiently. Owing to the absence of a 5' cap, Barley yellow dwarf virus (BYDV, Luteoviridae family) genomic and subgenomic RNA1 rely on a cap-independent translation element (BTE), which is an RNA domain capable of promoting translation. In contrast to an internal ribosome entry site (IRES), the BTE is located at the 3' end of the viral mRNA, while translation initiation occurs at the 5' end ...


Genome Rearrangement At Reversed-Ends Ds Element In Arabidopsis, Lakshminarasimhan Krishnaswamy Jan 2007

Genome Rearrangement At Reversed-Ends Ds Element In Arabidopsis, Lakshminarasimhan Krishnaswamy

Retrospective Theses and Dissertations

In course of McClintock's study on radiation induced chromosome breakage, she observed frequent chromosome break generated at a specific locus on chromosome 9. The precision of frequent breaks at this particular locus led to the discovery of Ac/Ds elements. In recent years, molecular characterization of Ac/Ds induced chromosome breaks reveal that when multiple transposon ends are in proximity, the transposase could recognize non-canonical transposon ends resulting in various kinds of chromosomal rearrangements including deletion, inversion, translocation, duplication and, sometimes, chromosome breaks. Previous studies in our lab show that in the maize P1-rr11 allele of the P1 gene ...


Advanced Detection And Separation Methods: Developments In Surface-Enhanced Raman Scattering Readout Immunoassays And Electrochemically Modulated Liquid Chromatography, Betsy Jean Yakes Jan 2007

Advanced Detection And Separation Methods: Developments In Surface-Enhanced Raman Scattering Readout Immunoassays And Electrochemically Modulated Liquid Chromatography, Betsy Jean Yakes

Retrospective Theses and Dissertations

This dissertation explores the use of novel detection methods for biological and chemical components commonly found in the environment. It encompasses two techniques: surface-enhanced Raman scattering (SERS) and electrochemically modulated liquid chromatography (EMLC).;Immunoassays using SERS as a readout tool have been developed in this laboratory and have shown low levels of detection (i.e., pico- to femtomolar and single binding event detection) for disease and biowarfare agents. This thesis seeks to further the performance of this platform for bacteria detection and explore strategies to increase the SERS response. Specifically, the first section of this dissertation focuses on the detection ...


Analysis Of Globulin-1 Promoter Activity And The Expression Of Gfp In Transgenic Maize Seed Tissues And Processing Of Gfp-Containing Grain, Colin Shepherd Jan 2007

Analysis Of Globulin-1 Promoter Activity And The Expression Of Gfp In Transgenic Maize Seed Tissues And Processing Of Gfp-Containing Grain, Colin Shepherd

Retrospective Theses and Dissertations

The maize (Zea mays L) kernel is perfectly configured to store proteins and nutrients. The major proteins of the maize kernel are known as seed storage proteins because they have no enzymatic function and they accumulate to a high level for use during germination of the seed as an immediate nutrient source. These seed storage proteins are responsible for much of the nutrient quality of maize when used as a food or feed source. Understanding the regulation of seed storage protein deposition may allow researchers to improve the use of maize grain in a variety of food and feed products ...


Effects Of Dietary Conjugated Linoleic Acid On European Corn Borer Survival, Growth, Fatty Acid Composition, And Fecundity, Lindsey Gereszek Jan 2007

Effects Of Dietary Conjugated Linoleic Acid On European Corn Borer Survival, Growth, Fatty Acid Composition, And Fecundity, Lindsey Gereszek

Retrospective Theses and Dissertations

No abstract provided.


Biophysical Characterization Of The 117 Amino Acids Long N-Terminal Segment Of D-Raf (Isoform A), Oren Tchaicheeyan Jan 2007

Biophysical Characterization Of The 117 Amino Acids Long N-Terminal Segment Of D-Raf (Isoform A), Oren Tchaicheeyan

Retrospective Theses and Dissertations

Drosophila Raf (D-Raf) is an orthologue of Human B-Raf. D-Raf may have two splice variants, a short (Isoform A, I-A) and a long one (Isoform B, I-B). Protocols for over-expression (E. Coli) and purification of 117 amino acids (aa) N-terminal segment (NTS) of D-Raf protein (I-A), both WT and Mutant (T60E) were established. These protein constructs were also biophysically characterized. Thrombin cleavage conditions were optimized, as monitored by 5 amino acid NTS sequencing, mass spectroscopy and SDS-PAGE electrophoresis. An attempt to purify the 160 aa D-Raf (I-B) NTS failed. NMR characterization in a 700 MHz magnet employing three experiments (HSQC ...


Allosteric Regulation Of Mammalian And Bacterial Fructose-1,6-Bisphosphatases, Justin Keith Hines Jan 2007

Allosteric Regulation Of Mammalian And Bacterial Fructose-1,6-Bisphosphatases, Justin Keith Hines

Retrospective Theses and Dissertations

Fructose-1,6-bisphosphatase (FBPase) is a critical regulatory enzyme in gluconeogenesis. In mammals, the enzyme is subject to metabolic regulation; the hormones insulin and glucagon reciprocally regulate the enzyme in the liver through the controlled production and degradation of the inhibitor fructose-2,6-bisphosphate (Fru-2,6-P2) and hence control hepatic glucose production. FBPase is nearly ubiquitous in living organisms and in the bacterium Escherichia coli , the homologous enzyme is imperative for growth on gluconeogenic substrates. How does this organism regulate FBPase without the use of exogenous hormones or Fru-2,6-P2? Investigations reported herein describe high resolution crystal structures of the enzyme from ...


Determinants Of Substrate Recognition And Mechanisms Of Subunit Exchange, Yang Zhou Jan 2007

Determinants Of Substrate Recognition And Mechanisms Of Subunit Exchange, Yang Zhou

Retrospective Theses and Dissertations

Adenylosuccinate synthetase (ADSS) offers a good opportunity to measure the effect of non-bonded interactions. Both IMP and 2'-deoxy-IMP are good substrates supporting identical binding affinities and maximal velocities; however, L-aspartate exhibits significant differences in binding affinities depending on which nucleotide is used as a substrate. Crystal structures of both complexes were identical except for the absence of the 2'-OH group in the dexoy-nucleotide complex. The decrease in energy (∼2 kcal/mole) in non-bonded interactions due to the loss of one atom explains the nearly 40-fold difference in the binding affinity of L-aspartate and an analog of L-aspartate called ...


Investigation Of Allosteric Regulation Of Porcine Fructose-1,6-Bisphosphatase, Jian Lu Jan 2007

Investigation Of Allosteric Regulation Of Porcine Fructose-1,6-Bisphosphatase, Jian Lu

Retrospective Theses and Dissertations

Porcine Fructose-1,6-bisphosphatase is a homotetramer with four identical subunits. It plays a central role in gluconeogenesis and is tightly regulated by metabolites fructose 2,6-bisphosphate (F26P2) and AMP. Loop 52-72, loop 182-194 and residues 7-11 of the N-terminal segment play important role in the mechanism of catalysis and allosteric inhibition by AMP by retaining a structural hydrophobic region. Disruption of the hydrophobic region will evidently affect the catalysis and regulation of FBPase. Mutation of Thr53 reduced the catalysis activity of FBPase, mutation of Thr194 eliminated inhibition of AMP due to its low affinity to the enzyme, while double mutation ...


Structural And Functional Studies On Membrane Fusion Proteins-Snares, Yibin Xu Jan 2007

Structural And Functional Studies On Membrane Fusion Proteins-Snares, Yibin Xu

Retrospective Theses and Dissertations

Membrane fusion is a ubiquitous and essential intracellular function. Membrane fusion is mediated by a protein superfamily known as N-ethymaleimide-sensitive fusion protein attachment protein receptors (SNAREs). From plant to mammal, there is a remarkable sequence similarity among the SNARE-family related to the conserved function of this family. The function of SNAREs has been proposed to be the key to membrane fusion as minimal fusion machinery. Depending on their distinct membrane distribution, SNAREs were classified into v (vesicle)-SNAREs and t (target)-SNAREs. It is widely believed that SNARE complex assembly by v- and t-SNAREs on opposite membranes offers the ultimate ...


Taxonomic Review, Phylogeny, And Geographical Population Structure Of The Sawfishes (Chondrichthyes, Pristiformes), Vicente Vieira Faria Jan 2007

Taxonomic Review, Phylogeny, And Geographical Population Structure Of The Sawfishes (Chondrichthyes, Pristiformes), Vicente Vieira Faria

Retrospective Theses and Dissertations

Sawfishes (Pristidae) are considered endangered species worldwide, but their conservation and management have been undermined due to poor understanding of the group's natural taxonomy. The main purpose of this work was to review the taxonomy of sawfishes based on historical taxonomic literature supplemented with empirical observations on morphology, genetics, and geographic distribution. A molecular phylogenetics study based on both mitochondrial and nuclear genes was carried out to estimate the evolutionary interrelationships among the different species of sawfishes. Seven distinct living species are recognized based on external morphology and DNA sequence comparisons. Four are distributed in the Indo-West Pacific, two ...


Structural Study Of Yeast Snare Proteins On Membrane By Epr, Yinghui Zhang Jan 2007

Structural Study Of Yeast Snare Proteins On Membrane By Epr, Yinghui Zhang

Retrospective Theses and Dissertations

Membrane fusion is a basic biophysical process involved in cell's physiological activities. The value of studying the exocytosis of mammalian cells and yeast cells lies in that a highly conserved SNARE (Soluble N-ethylmaleimide-sensitive factor attachment protein receptor) protein super family mediates this process and is proposed to be the core protein machinery for the membrane. The SNARE proteins located on plasma membrane and vesicle membrane are assembled together to form a trans-SNARE bridging two separate membranes and initiating membrane.;My research centers on how SNARE assembly involved in yeast cells' exocytosis promotes membrane fusion between yeast vesicle and plasma ...


Kinetic Studies Of Escherichia Coli And Human Saicar Synthetase, Daniel Joseph Binkowski Jan 2007

Kinetic Studies Of Escherichia Coli And Human Saicar Synthetase, Daniel Joseph Binkowski

Retrospective Theses and Dissertations

Phosphoribosyl-aminoimidazole-succinocarboxamide synthetase (SAICAR synthetase) catalyzes the eighth step of de novo IMP biosynthesis in bacteria, and the seventh step in humans. The SAICAR synthetase reaction is analogous to that of adenylosuccinate synthetase (first committed step of AMP biosynthesis), using ATP to ligate 5-amino-imidazole-4-carboxy ribonucleotide and L-aspartate to produce 5-aminoimidazole-4(N-succinylcarboxamide) ribonucleotide (SAICAR). SAICAR synthetase and other enzymes of purine nucleotide biosynthesis are targets of natural products that impair cell growth. Prior to these studies, the kinetic mechanism of any SAICAR synthetase was unknown. Herein are reported the kinetic mechanisms of bacterial and human SAICAR synthetase activities, the structure of the ...


The Role Of The Integrase C-Terminus In Replication And Targeted Integration Of The Yeast Retrotransposon Ty5, Troy Leon Brady Jan 2007

The Role Of The Integrase C-Terminus In Replication And Targeted Integration Of The Yeast Retrotransposon Ty5, Troy Leon Brady

Retrospective Theses and Dissertations

Mobile genetic elements have the inherent potential to shape the genomes of their hosts. The retroelements, which include the retrotransposons and retroviruses, are a particularly abundant class of mobile elements. The site of retroelement insertion in the host genome is largely determined by the element-encoded protein integrase (IN). Research presented here addresses three aspects of IN from the retrotransposon Ty5: the function of a conserved domain (GKGY) in the IN C-terminus, the role of IN post-translational modification in targeted integration, and the origin of the Ty5 targeting mechanism.;Sequence analysis of a conserved domain (GKGY) found in the C-termini of ...


Crystallographic Analysis Of Parasponia Andersonii Hemoglobin, Andrea Savage Jan 2007

Crystallographic Analysis Of Parasponia Andersonii Hemoglobin, Andrea Savage

Retrospective Theses and Dissertations

The x-ray crystal structure of ferric Parasponia andersonii hemoglobin has been determined to 2.3 A resolution by molecular replacement with Rice hemoglobin 1 (rHb1). By means of absorbance spectroscopy, electrochemistry and x-ray crystallography, it has been revealed that depending on the oxidation state, the heme iron can exsist in either the pentacoordinate or hexacoordinate form. Pentacoordination of the heme iron is found primarily in the deoxy ferrous (2+) form, whereas the ferric (3+) form is exhibited predominantly in bis-histidine coordination. This is unique among known forms of hemoglobins. Furthermore, the crystallographic data has provided evidence which supports previous works ...


Characterization Of The Starch Branching Enzymes In Arabidopsis Thaliana, Qinglei Gan Jan 2007

Characterization Of The Starch Branching Enzymes In Arabidopsis Thaliana, Qinglei Gan

Retrospective Theses and Dissertations

The roles of three starch branching enzyme isoforms, BE1, BE2 and BE3, in Arabidopsis leaf starch metabolism were investigated by AtBE mutant analysis. We also determined the expression patterns of BEs in Arabidopsis by GUS staining and microarray experiments. The possible correlation of BE gene expression with other genes in the Arabidopsis genome was analyzed to identify putative genes that are functionally correlated with BEs. Motifs in the promoters of the Arabidopsis BE genes were identified to better understand their expression differences. BE2 and BE3 expression levels during a diurnal cycle were evaluated with isoform specific antibodies. Meantime, the in ...


Role Of Leu[Superscript B 10] In Cytoglobin And Comparison Of Direct And Indirect Measurements Of Hexacoordinate Hemoglobin Ligand Affinity, Jordan Richard Witmer Jan 2007

Role Of Leu[Superscript B 10] In Cytoglobin And Comparison Of Direct And Indirect Measurements Of Hexacoordinate Hemoglobin Ligand Affinity, Jordan Richard Witmer

Retrospective Theses and Dissertations

Hexacoordinate hemoglobins (HxHbs) have recently been discovered and have been found in nearly all living organisms. In humans, hxHbs are represented by neuroglobin (Ngb) and cytoglobin (Cgb). While Ngb is principally found in the brain, Cgb is ubiquitously expressed in all tissues. Ligand binding in hexacoordinate hemoglobins is complex due to the reversible coordination of the heme iron by the distal histidine. Hexacoordination regulates ligand binding and is regulated by specific residues in the heme pocket including the B10 position. Therefore, we investigated the influence of LeuB10 on hexacoordination and ligand binding in Cgb. Ligand binding was analyzed for recombinant ...


Biochemical Studies Of Mms6--A Biomineralization Protein, Lijun Wang Jan 2007

Biochemical Studies Of Mms6--A Biomineralization Protein, Lijun Wang

Retrospective Theses and Dissertations

Magnetotactic bacteria are aquatic prokaryotes that can move under the direction of a local geomagnetic field. The magnetosomes found in magnetotactic bacteria are arranged in a single chain or chains, which is the physics basis of magnetotaxis trait. It is believed that with the trait of magnetotaxis, magnetotactic bacteria can simplify their search for the optimal oxygen environments.;Mms6 is a magnetosome membrane-associated protein in Magnetospirillum magneticum strain AMB-1. It has 59 amino acids with a hydrophobic N-terminal and a hydrophilic C-terminal region. Magnetite nanoparticles with similar size and shape to bacterial magnetite can be synthesized in vitro in the ...


The Role Of Tyr61 In Synechocystis Hemoglobin In Regulating E-Helix Movement, Anand Venugopal Jan 2007

The Role Of Tyr61 In Synechocystis Hemoglobin In Regulating E-Helix Movement, Anand Venugopal

Retrospective Theses and Dissertations

Hexacoordinate hemoglobins have been discovered that have yet to have a functional role confidently assigned to them. The eubacteria synechocystis sp. PCC 6803 has been found to have a truncated hemoglobin which is hexacoordinated. The structures of the hexacoordinated and cyanide bound forms of synechocystis hemoglobin have been solved, and a large shift in the E-helix has been seen upon ligand binding. Also, the loop connecting the E and F helix contains residues Tyr61 and Asp62 that are involved in hydrogen bonding networks in the cyanide-bound and hexacoordinate states respectively. A mutation was made to the Y61 residue to make ...


Biophysical And Biochemical Characterization Of Hexacoordinate Hemoglobins, Benoit Joel Smagghe Jan 2007

Biophysical And Biochemical Characterization Of Hexacoordinate Hemoglobins, Benoit Joel Smagghe

Retrospective Theses and Dissertations

Within the hemoglobin (Hb) superfamily, an unusual group called hexacoordinate hemoglobin (hxHbs) whose members are found in all living organisms is the subject of exciting studies since their discovery. They are named "hexacoordinate" because of reversible coordination of the sixth coordination site of the heme iron by a histidine side chain located in the heme distal pocket. Interestingly, hxHbs share high degree of similarity in their primary sequence and a common globin fold indicating that they might share a common role and that endogenous coordination might be an important part of their not yet characterized physiological function. The characterization of ...


Biotechnological Improvements Of Bio-Based Fuels And Lubricants: Characterization Of Genes For Branched Chain Fatty Acids And Mono-Acyl Esters, Yuqin Jin Jan 2007

Biotechnological Improvements Of Bio-Based Fuels And Lubricants: Characterization Of Genes For Branched Chain Fatty Acids And Mono-Acyl Esters, Yuqin Jin

Retrospective Theses and Dissertations

The goals of the research in this dissertation are to generate the fundamental technologies that can be used to improve the production and quality of vegetable-oil based biodiesel and biolubricants. Specifically, we sought to test the hypothesis that branched chain fatty acids (BCFAs) can substitute for unsaturated fatty acids in maintaining fluidityin an organism. Toward this goal, we examined the utility of two Bacillus keto-acyl synthase III (KASIII) coding genes (yhfB and yjaX) in producing BCFAs. In conducting this research it was necessary to examine if the E. coli KASIII gene (fabH) was essential for initiating fatty acid biosynthesis and ...


Investigating Reactions Catalyzed By Terpene Synthases In A Novel Model System, Paul Ross Wilderman Jan 2007

Investigating Reactions Catalyzed By Terpene Synthases In A Novel Model System, Paul Ross Wilderman

Retrospective Theses and Dissertations

Terpene synthases are a set of enzymes that initiate the biosynthetic production of the largest class of known natural products. However, not much is known about how these enzymes carry out their complex cyclization reactions to form polycyclic olefins from linear hydrocarbon diphosphates such as geranylgeranyl diphosphate.;Since rice is known to produce many diterpenoid natural products and the rice genome was recently published, sequence information for many putative diterpene synthases was available. This provided the basis for functional characterization of the diterpene synthases from rice (Oryza sativa ssp. Indica). An enzyme producing syn-pimaradiene was identified, followed by those producing ...


Repair Of Endonuclease- And Transposon-Induced Dna Double Strand Breaks By Host Factors In Arabidopsis , Zhuying Li Jan 2006

Repair Of Endonuclease- And Transposon-Induced Dna Double Strand Breaks By Host Factors In Arabidopsis , Zhuying Li

Retrospective Theses and Dissertations

The maize Ac/Ds transposable elements are members of the hAT transposon family. Ac/Ds transpose in diverse species and via a mechanism similar to that involved in V(D)J recombination. Here, we studied the repair of Ds transposition-induced hairpins by host factors in Arabidopsis. The results indicate that AtKu could position hairpin-opening to sites close to the hairpin apex. In the absence of AtMre11 or AtKu70, repair junctions have a higher frequency of small deletions (3-10bp) and larger deletions (11-50bp), respectively. Comparison of the repair of I-SceI- and Ds-induced DSB indicates that Ac transposase could influence Ds-induced DSB ...


Enhancement Of Plant Performance And Biochemical Constituents In Field And Hairy Root Production Of Echinacea , Fredy Rolando Romero Orellana Jan 2006

Enhancement Of Plant Performance And Biochemical Constituents In Field And Hairy Root Production Of Echinacea , Fredy Rolando Romero Orellana

Retrospective Theses and Dissertations

Echinacea is a commercially important source of natural products and phytopharmaceuticals. Echinacea products currently constitute a significant portion of the rapidly growing, multi-billion dollar natural products market. This popularity has led to the expansion of commercial cultivation of Echinacea and the need to find alternative methods to meet the increasing demands of the phytopharmaceutical market. Two methods of producing biochemical constituents in commercial use include extraction from field-grown plants and in vitro hairy root production;Echinacea seeds in the wild typically show a high degree of dormancy. In order to enhance Echinacea seed germination for field production, alternative methods to ...


The Mechanism Of Arabidopsis Immutans Variegation , Aigen Fu Jan 2006

The Mechanism Of Arabidopsis Immutans Variegation , Aigen Fu

Retrospective Theses and Dissertations

The Arabidopsis immutans (im) is variegated with green sectors and white sectors containing defective plastids due to a nuclear gene mutation. IM is a plastid terminal oxidase (PTOX) sharing similarity with mitochondrial alternative oxidase (AOX). In order to better understand IM's biological roles and variegation mechanism of im, I characterized im plants at different levels;A structural model of IM, in which the diiron reaction center is composed of two conserved histidine and four glutamate residues, was tested by mutagenesis in vitro and in planta. It that these six conserved residues were found essential for IM activity and do ...


Studies Of Hypoxia Response And Regulation Of Hypoxia-Inducible Factor Hif-1 In Caenorhabditis Elegans , Chuan Shen Jan 2006

Studies Of Hypoxia Response And Regulation Of Hypoxia-Inducible Factor Hif-1 In Caenorhabditis Elegans , Chuan Shen

Retrospective Theses and Dissertations

All aerobic organisms require molecular oxygen to generate metabolic energy for normal growth and survival. During evolution, multi-cellular organisms have developed and refined complex networks for adaptation to hypoxic environments at both systemic and cellular levels. Adaptation to hypoxia largely results from changes in the activity and expression of key proteins. These include proteins involved in increasing oxygen delivery to hypoxic tissues and proteins that facilitate glycolysis for anaerobic metabolism. The mammalian transcription factor hypoxia-inducible factor (HIF) is a master regulator of oxygen homeostasis. More than 100 target genes of HIF mediate broad systemic and local responses to hypoxia, including ...


The Analysis Of Global Gene Expression Related To Starch, Lipid And Protein Composition , Ling Li Jan 2006

The Analysis Of Global Gene Expression Related To Starch, Lipid And Protein Composition , Ling Li

Retrospective Theses and Dissertations

This dissertation provides a greater understanding of starch metabolism and its regulation by using mutants altered in starch accumulation, together with a combination of approaches such as global gene profiling, promoter::GUS/GFP analyses, and genetics;Starch debranching enzymes are critical for starch accumulation. The temporal and spatial co-expression of ISA1 and ISA2 is consistent with previous evidence that they co-function as a protein complex (ISA1/ISA2) for starch synthesis. The extensive temporal and spatial co-expression of ISA2 and ISA3 suggests they might also form a protein complex, taking part in starch metabolism. ISA3 protein is localized in leaf chloroplast ...


Plant Genome Informatics: Evaluation And Analysis Of Genomic Dna Features Involved In The Transcriptional Processing Of Protein Coding Genes , Shannon Dwayne Schlueter Jan 2006

Plant Genome Informatics: Evaluation And Analysis Of Genomic Dna Features Involved In The Transcriptional Processing Of Protein Coding Genes , Shannon Dwayne Schlueter

Retrospective Theses and Dissertations

As biological data collection methods have become more cost effective and less time consuming, the necessity for computational tools to store, manage, and analyze this data has led to the creation of a broad field of research. Bioinformatics, while firmly rooted in the technology of information management, is now a mainstream component in the majority of scientific investigations. With the vast majority of effort in bioinformatics being applied to research on vertebrate species, researchers in the plant sciences have often been left with less than satisfactory tools. The research presented in this dissertation was done in an effort to advance ...


Recognition And Signal Transduction In The Expression Of Phytophthora Resistance In Soybean [Glycine Max (L) Merr] , Hongyu Gao Jan 2006

Recognition And Signal Transduction In The Expression Of Phytophthora Resistance In Soybean [Glycine Max (L) Merr] , Hongyu Gao

Retrospective Theses and Dissertations

Phytophthora sojae, a hemibiotrophic oomycete, is the causal pathogen of stem and root rot disease of soybean (Glycine max [L.] Merr). The annual soybean yield loss suffered from this disease is valued at about 300 million dollars. A series of single Rps genes protect soybean from the root and stem rot disease. In the last two decades Rps1-k has been the most stable and widely used Phytophthora resistance gene in the major soybean-producing regions of the United States;Two classes of CC-NB-LRR-type Rps1-k genes were isolated from the Rps1-k locus. Representative members from each gene class were expressed in transgenic ...