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Articles 1 - 12 of 12

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Reversible Palmitoylation Of H-Ras Is Affected By Oxidants And Activation State , Tara Lynn Baker Jan 1998

Reversible Palmitoylation Of H-Ras Is Affected By Oxidants And Activation State , Tara Lynn Baker

Retrospective Theses and Dissertations

Signal transduction organizes cell growth, differentiation, and apoptosis. One component of several signaling pathways leading to these outcomes is the Ras protein. Ras is a small GTPase, binding GTP in an active conformation and GDP in an inactive conformation. Upon nucleotide binding, the conformation of Ras changes so that its effector region can bind to proteins directly downstream in several pathways. Several GTP-bound mutants have been discovered, and an activated Ras mutant has been found in at least 50% of human tumors;Ras must reside at the membrane in order to interact with effector proteins. Binding at the plasma membrane ...


Cloning And Characterization Of A Soybean Snf2 Yeast Homolog , Weiping Chen Jan 1997

Cloning And Characterization Of A Soybean Snf2 Yeast Homolog , Weiping Chen

Retrospective Theses and Dissertations

It is of great economic and scientific interest to understand plant disease resistance genes at the molecular level. Phytophthora sojae, a fungal pathogen of soybean is of particular interest since the root and stem rot resulting from its infection causes great economic loss. It is the goal of this project to clone Phytophthora resistance genes from soybean. This was done by designing primers from the conserved regions of known plant disease resistance genes using a modified differential display for near isogenic lines; L85-3044 a resistant line and Williams, a susceptible line. Several polymorphic bands were identified, including a unique 0 ...


Characterization Of Cis-Acting Replication Control Elements In Ribosomal Rna Genes Of Tetrahymena Thermophila , Wen-Ling Shaiu Jan 1993

Characterization Of Cis-Acting Replication Control Elements In Ribosomal Rna Genes Of Tetrahymena Thermophila , Wen-Ling Shaiu

Retrospective Theses and Dissertations

This dissertation describes the identification and characterization of specific cis-acting replication control elements in the replication origin of Tetrahymena rDNA. Earlier studies of a mutant rDNA allele, C3-rmm1, identified a cis-acting sequence (the Type I repeat) within the 5[superscript]' non-transcribed spacer of rDNA that affects rDNA replication (Larson et al. 1986). Characterization of two additional, independently-isolated mutants (C3-rmm3 and C3-rmm4) and a revertant of C3-rmm4 strongly argue that the Type I repeat is indeed a cis-acting replication control element. Mutations causing rDNA replication defects were identified in copies of the Type I repeat that are separated by >600 bp ...


Characterization Of Dna-Binding Proteins That Recognize The Replication Origin Of The Rrna Genes Of Tetrahymena Thermophila , Angela Rae Umthun Jan 1993

Characterization Of Dna-Binding Proteins That Recognize The Replication Origin Of The Rrna Genes Of Tetrahymena Thermophila , Angela Rae Umthun

Retrospective Theses and Dissertations

Proteins that bind to specific sequences in replication origins have been shown to be important components of both prokaryotic and eukaryotic DNA replication systems. An origin of DNA replication is located within the 5[superscript]' non-transcribed spacer region of the rRNA genes (rDNA) of Tetrahymena thermophila. Previous work has shown that several cis-acting mutations responsible for rDNA replication defects lie in upstream repeats of a transcriptional promoter element, the Type I repeat;Using FE(II)EDTA cleavage footprinting and gel-mobility shift assays, I identified and characterized two distinct DNA-binding activities that interact with the Type I repeat. One of these ...


Direct Introduction Of Dna Into Embryonic Axes Of Glycine Max By Microprojectile Bombardment , Charisse Marie Buising Jan 1992

Direct Introduction Of Dna Into Embryonic Axes Of Glycine Max By Microprojectile Bombardment , Charisse Marie Buising

Retrospective Theses and Dissertations

Apical meristem and axillary bud development in germinating soybean embryonic axes treated for 24 hr with the cytokinin, 6-benzylaminopurine (BAP) seemed normal morphologically, but was delayed by about 9 to 15 hr. After 5 days, numerous, rapidly proliferating meristematic regions (de novo vegetative buds) were observed in BAP-treated axes around the perimeter of the apical dome. These visible changes were preceded by transient inhibition of DNA synthesis in the shoot apices as early as 21-36 hr post-imbibition;Some of the new meristematic regions developed into multiple shoots, which were excised, subcultured, and used to generate mature plants. In the absence ...


Molecular Characterization Of The Yeast Gene Hsp60 , Donald Stevenson Reading Jan 1990

Molecular Characterization Of The Yeast Gene Hsp60 , Donald Stevenson Reading

Retrospective Theses and Dissertations

In all organisms studied, heat shock or other stressful stimuli lead to profound changes in gene expression. During heat shock, a small group of evolutionarily conserved proteins, termed heat shock proteins (hsps), are synthesized in abunDance; Several hsps are known to have vital cellular function even at normal temperature. These hsps have the capacity to bind polypeptide chains and have a probable role in the folding, translocation, and assembly pathways of many proteins. One such protein, hsp60, is located within mitochondria, is highly conserved in evolution, and based on immunological and physical characterization, is a member of a family of ...


Aspects Of Regulation Of Ketoglutarate Dehydrogenase Activity From Ribbed Mussel (Modiolus Demissus) Gills And Cauliflower (Brassica Oleracea) , George Antun Karam Jan 1989

Aspects Of Regulation Of Ketoglutarate Dehydrogenase Activity From Ribbed Mussel (Modiolus Demissus) Gills And Cauliflower (Brassica Oleracea) , George Antun Karam

Retrospective Theses and Dissertations

In ribbed bivalve mollusc gills, the enzyme ketoglutarate dehydrogenase is regulated by pH, ketoglutarate and adenine nucleotides but not by calcium ions. In cauliflower, the enzyme is regulated by variations in Ca[superscript] 2+, Mg[superscript] 2+, adenine nucleotides and ketoglutarate. In plants, the decrease in mitochondrial Ca[superscript] 2+ inhibits KGDH whereas the entry of Ca[superscript] 2+ reactivates the enzyme. No such regulation appears to exist with the ribbed mussel KGDH.


The Metabolism And Localization Of Two Proteins During Heat Shock Of Tetrahymena Thermophila , Thomas William Mcmullin Jan 1987

The Metabolism And Localization Of Two Proteins During Heat Shock Of Tetrahymena Thermophila , Thomas William Mcmullin

Retrospective Theses and Dissertations

When T. thermophila is subjected to hyperthermal stress, it immediately but transiently represses the synthesis of the normal array of proteins (non-hsps) and induces the synthesis of the heat shock protein (hsps). These changes in protein synthetic patterns are controlled at both the transcriptional and translational levels;To determine whether the translational regulatory events could be correlated with changes in the protein synthetic machinery, heat shock induced modifications of the ribosomal proteins were investigated. Of particular interest was the heat shock induced association of a 22 kDa protein, p22, with the small subunit of the ribosome. The synthesis of p22 ...


A Novel Insulin From The Bullfrog: Its Structure And Function In Protein Secretion By Hepatocytes , Jocelyn Jean Hulsebus Jan 1987

A Novel Insulin From The Bullfrog: Its Structure And Function In Protein Secretion By Hepatocytes , Jocelyn Jean Hulsebus

Retrospective Theses and Dissertations

Bullfrog insulin was extracted and purified from the pancreas of Rana catesbeiana adults using gel filtration and reverse phase high performance liquid chromatography. Amino acid analysis of bullfrog insulin revealed 52 amino acids instead of the most common number of 51. Hagfish insulin is the only other known insulin with 52 amino acids. The most unique feature of bullfrog insulin is a two amino acid extension on the amino terminus (A1) of the A chain. This is the only insulin to date that has an extension at this position. In mammals, the deletion of A1 glycine or addition of a ...


Interaction Of Tgf-[Beta], Egf, And Tpa In Their Effects On The Production Of Secreted Proteins By Mouse 3t3 Cells And Human Fibroblasts , Chia-Ping Chiang Jan 1986

Interaction Of Tgf-[Beta], Egf, And Tpa In Their Effects On The Production Of Secreted Proteins By Mouse 3t3 Cells And Human Fibroblasts , Chia-Ping Chiang

Retrospective Theses and Dissertations

Growth regulators such as epidermal growth factor (EGF), fibroblast growth factor (FGF), 12-O-tetradecanoylphorbol 13-acetate (TPA), and type (beta) transforming growth factor (TGF-(beta)) regulate the production of certain proteins by cells in culture. EGF and TPA increased the secreted and intracellular levels of Mitogen-Regulated Protein (MRP) and Major Excreted Protein (MEP) by Swiss 3T3 cells. MRP is related by sequence to prolactin. MEP is a thiol protease located intracellularly in the lysosomes. EGF, FGF, and TPA also selectively induced a 52,000-dalton Mitogen-Induced Protein (MIP52) secreted by human fibroblasts. Two types of TGF-(beta)s were tested for their effects ...


The Heat Shock Response In Tetrahymena Thermophila: Control Of Protein Synthesis , Kevin W. Kraus Jan 1986

The Heat Shock Response In Tetrahymena Thermophila: Control Of Protein Synthesis , Kevin W. Kraus

Retrospective Theses and Dissertations

The changes in protein synthesis which result when Tetrahymena thermophila cells are shifted from 30(DEGREES)C to 40(DEGREES)C are shown to be regulated at both the mRNA accumulation and mRNA translation levels. The heat shock protein mRNAs are preferentially translated early in a heat shock while non-heat shock protein messages remain in the cell in a non-translated state. Later in heat shock these non-hsp messages return to polysomes at the same time as a small heat-induced RNA becomes associated with polysomal ribosomes. This novel RNA species may play an important role in the return to normal protein ...


Cyclic Nucleotide Levels In The Mammary Glands Of Rats During Late Pregnancy And Early Lactation , Fredrick William George Jan 1975

Cyclic Nucleotide Levels In The Mammary Glands Of Rats During Late Pregnancy And Early Lactation , Fredrick William George

Retrospective Theses and Dissertations

No abstract provided.