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Biochemistry, Biophysics, and Structural Biology Commons

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Retrospective Theses and Dissertations

Physical chemistry

2004

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Protein-Protein Interaction And Electron Transfer: Case Of Cytochrome F And Cytochrome C6 , Tijana Žarković Grove Jan 2004

Protein-Protein Interaction And Electron Transfer: Case Of Cytochrome F And Cytochrome C6 , Tijana Žarković Grove

Retrospective Theses and Dissertations

We used photo-induced reaction as a tool to look to explore dynamics of the protein-protein interaction between cytochrome f and cytochrome c6 from C. reinhardtii. In our studies of electron transfer between 3Zncyt c6 and cyt f(III) we discovered two distinct intracomplex processes corresponding to electron transfer within diprotein complexes termed persistent and transient. The overall reaction remained biphasic and the rate constants for both processes were invariant to the change of ionic strength within the studied interval. The docking of these proteins is due to strong hydrophobic interaction slightly augmented by weak electrostatic attraction. We used covalent cross-linking ...