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Biochemistry, Biophysics, and Structural Biology Commons

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Retrospective Theses and Dissertations

2006

Biomolecular science

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Chemical Reagents In The Study Of Biomolecules , Laura-Mirela Dutcă Jan 2006

Chemical Reagents In The Study Of Biomolecules , Laura-Mirela Dutcă

Retrospective Theses and Dissertations

Chemical reagents are an important tool in the study of biological systems. In this thesis some applications of chemical reagents in the study of biomolecules are presented. Selective cleavage of proteins is a common procedure in many biochemical applications ranging from standard protein sequencing to novel methods in proteomics and bioengineering. Pt(II) complexes are synthetic proteases, with the same selectivity as cyanogen bromide, they cleave at the C-terminus of methionine residues. Pt(II) reagent has several advantages over cyanogen bromide. For example, cyanogen bromide irreversibly converts methionine residues to serine lactone in 70% formic acid, while Pt(II) reagents ...