Articles 1 - 1 of 1
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
The Mechanism Of Integration Preference To Heterochromatin Of Yeast Retrotransposon Ty5 , Weiwu Xie
Retrospective Theses and Dissertations
Ty5 is a retrotransposon in Saccharomyces cerevisiae. In wild type yeast strains, over 90% of Ty5 insertions occur at the telomeres or HM loci. These regions are bound in silent chromatin, which is analogous to heterochromatin of higher eukaryotes. Our laboratory previously described a targeting mutant of Ty5 that has an amino acid substitution near the C-terminus of integrase. Targeting to silent chromatin is reduced more than 20-fold in the mutant. We further defined the Ty5 targeting domain (TD) by saturation mutagenesis. All of the targeting mutations mapped to a stretch of six amino acids (LDSSPP). We tethered TD to ...