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Articles 1 - 7 of 7

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Protein Structure, Distribution Of Homoplasy And Phylogenetic Inference , Olivier Fedrigo Jan 2003

Protein Structure, Distribution Of Homoplasy And Phylogenetic Inference , Olivier Fedrigo

Retrospective Theses and Dissertations

All molecular phylogenetic methods are based on two assumptions: independence of characters and constant selective pressure. It is well known that these assumptions are often violated, but it is assumed that the methods are robust to such violations. However, the increase of unorthodox phylogenies makes us wonder about their reliability. None of the currently used phylogenetic methods and models account for a change of functional/structural constraint at the protein level. In order to understand the consequences on the phylogenetic inference, I studied the distribution of the structural/functional misleading phylogenetic signal in the tree and described the effect of ...


Characterization Of Two Novel Spindle Matrix Proteins In Drosophila, Chromator And Megator , Dong Wang Jan 2003

Characterization Of Two Novel Spindle Matrix Proteins In Drosophila, Chromator And Megator , Dong Wang

Retrospective Theses and Dissertations

A spindle matrix has been proposed to help organize and stabilize microtubules during mitosis. This concept has gained new interest due to the identification of Skeletor as a potential component of the spindle matrix. Skeletor is associated with chromosomes during interphase but reorganizes into a spindle-like structure during early prophase before the nuclear envelope breaks down and microtubules enter the nucleus. The Skeletor spindle extends from pole to pole during metaphase coaligning with the microtubule spindle and persists even after nocodazole treatment dissembles the microtubules. These features make Skeletor an ideal candidate as a spindle matrix protein.;In this study ...


Identification And Functional Analysis Of Thylakoid Membrane Proteome , Yingchun Wang Jan 2003

Identification And Functional Analysis Of Thylakoid Membrane Proteome , Yingchun Wang

Retrospective Theses and Dissertations

Membrane proteins play crucial roles in many biological functions. Identities and functions of most membrane proteins remain to be revealed. New technological breakthroughs in proteomics together with the availability of genomic sequence information make it possible to study functions of membrane proteins on a genome-wide scale. We used a multidisciplinary approach combining biochemistry, genetics, proteomics and bioinformatics to study the functions of the thylakoid proteome of Synechocystis sp. PCC6803. The thylakoid membrane proteins were separated into peripheral and integral fractions and resolved into 2-D gels with different pH ranges. The protein spots in the 2-D gels were subjected to peptide ...


Genetic And Molecular Characterization Of Maize Starch Debranching Enzymes , Jason Robert Dinges Jan 2003

Genetic And Molecular Characterization Of Maize Starch Debranching Enzymes , Jason Robert Dinges

Retrospective Theses and Dissertations

Starch is the primary carbohydrate reserve of plants, a significant source of food in the human diet, and an important industrial raw material. The arrangement of linear chains and branch linkages within amylopectin is responsible for the highly conserved architecture of the starch granule. This structure allows for efficient packaging of glucose and results in the unique functional properties of starch. It is known that starch synthases and branching enzymes catalyze alpha(1→4) chain elongation and introduction of alpha(1→6) branch linkages, respectively, but studies in several species suggests that starch debranching enzymes (DBE) are also involved in ...


Herpes Virus Icp47 And Us11 Suppression Of Ctl Activity Adapted For Use In Gene Therapy , Thomas Joseph Radosevich Jan 2003

Herpes Virus Icp47 And Us11 Suppression Of Ctl Activity Adapted For Use In Gene Therapy , Thomas Joseph Radosevich

Retrospective Theses and Dissertations

A major stumbling block encountered in gene therapy clinical trials has been the rapid destruction of the therapeutic product by the host's immune response. The goal of this work is to diminish the host Cytotoxic T Lymphocyte (CTL) response to gene therapy transgenes and transduced cells by incorporating immunosuppression genes into gene therapy vectors. The processing and presentation of antigens through the class I Major Histocompatibility Complex (class I MHC) pathway is known to be an early response to viral infection. Disruption of the class I MHC pathway results in a decrease of the CD8+ CTL response and prolongs ...


The Role Of Rev-Sr Protein Interactions In The Regulation Of Equine Infectious Anemia Virus Replication , Gregory Saang Park Jan 2003

The Role Of Rev-Sr Protein Interactions In The Regulation Of Equine Infectious Anemia Virus Replication , Gregory Saang Park

Retrospective Theses and Dissertations

Equine infectious anemia virus (EIAV) is a member of the lentivirus subfamily of retroviruses that produces a variable clinical disease course characterized as acute, chronic, and inapparent. The clinical signs can vary according to the stage of disease, and generally correlate with levels of virus replication. As with other retroviruses, EIAV utilizes both RNA and proteins to produce alternatively spliced transcripts required for virus replication. EIAV encodes a protein called Rev, which functions by binding unspliced and singly spliced viral mRNAs in the nucleus at a sequence called the Rev responsive element (RRE) and exporting them into the cytoplasm. Rev ...


Posttranslational Processing And Functional Analysis Of Tractin, An L1 Family Member In Leech , Yingzhi Xu Jan 2003

Posttranslational Processing And Functional Analysis Of Tractin, An L1 Family Member In Leech , Yingzhi Xu

Retrospective Theses and Dissertations

During neurogenesis, glycosylated cell adhesion molecules (CAMs) mediate the growth cone navigation and synapse formation. The structure of neuronal CAMs of Ig superfamily is characterized by the variability and complexity of their extracellular region which contain multiple tandemly arranged domains. The diversity in the structure of the neuronal CAMs is generated by alternative splicing and posttranslational modification, such as differential glycosylation and proteolytic processing.;Tractin is a member of the L1-family of neuronal CAMs in leech. It contains six Ig domains, 4 FNIII-like domains, an acidic domain, 12 repeats of a novel proline- and glycine-rich motif (PG/YG) with sequence ...