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Biochemistry, Biophysics, and Structural Biology Commons

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Retrospective Theses and Dissertations

2003

Electrical and computer engineering

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Identification And Functional Analysis Of Thylakoid Membrane Proteome , Yingchun Wang Jan 2003

Identification And Functional Analysis Of Thylakoid Membrane Proteome , Yingchun Wang

Retrospective Theses and Dissertations

Membrane proteins play crucial roles in many biological functions. Identities and functions of most membrane proteins remain to be revealed. New technological breakthroughs in proteomics together with the availability of genomic sequence information make it possible to study functions of membrane proteins on a genome-wide scale. We used a multidisciplinary approach combining biochemistry, genetics, proteomics and bioinformatics to study the functions of the thylakoid proteome of Synechocystis sp. PCC6803. The thylakoid membrane proteins were separated into peripheral and integral fractions and resolved into 2-D gels with different pH ranges. The protein spots in the 2-D gels were subjected to peptide ...