Open Access. Powered by Scholars. Published by Universities.®

Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Retrospective Theses and Dissertations

2003

Plant Sciences

Articles 1 - 5 of 5

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Investigation Of Iron-Deficiency Chlorosis In Soybean , Dirk Victor Charlson Jan 2003

Investigation Of Iron-Deficiency Chlorosis In Soybean , Dirk Victor Charlson

Retrospective Theses and Dissertations

Iron-Deficiency Chlorosis (IDC) causes yield losses in soybean and other crop species as a result of the plant's inability to efficiently acquire iron from calcareous soils. Although plants differ in their response to iron deficiency and IDC resistance has been improved in soybean, the biology of IDC is not well understood. We undertook an interdisciplinary approach towards increasing our knowledge of IDC resistance and iron acquisition strategies in higher plants. Firstly, we examined the usefulness of molecular markers to improve breeding efficiency for IDC resistance in soybean. After examining Simple Sequence Repeats (SSR) markers genetically linked to previously identified ...


Genetic And Molecular Characterization Of Maize Starch Debranching Enzymes , Jason Robert Dinges Jan 2003

Genetic And Molecular Characterization Of Maize Starch Debranching Enzymes , Jason Robert Dinges

Retrospective Theses and Dissertations

Starch is the primary carbohydrate reserve of plants, a significant source of food in the human diet, and an important industrial raw material. The arrangement of linear chains and branch linkages within amylopectin is responsible for the highly conserved architecture of the starch granule. This structure allows for efficient packaging of glucose and results in the unique functional properties of starch. It is known that starch synthases and branching enzymes catalyze alpha(1→4) chain elongation and introduction of alpha(1→6) branch linkages, respectively, but studies in several species suggests that starch debranching enzymes (DBE) are also involved in ...


Molecular And Genetic Characterization Of The Inheritance And Expression Of The Synthetic Porcine Alpha-Lactalbumin Transgene In Maize (Zea Mays L) , Earl H. Bicar Jan 2003

Molecular And Genetic Characterization Of The Inheritance And Expression Of The Synthetic Porcine Alpha-Lactalbumin Transgene In Maize (Zea Mays L) , Earl H. Bicar

Retrospective Theses and Dissertations

A codon-adjusted version of a gene encoding the porcine milk protein alpha-lactalbumin was synthesized to create maize with improved nutritional quality. Three expression vectors (P64, P45, P57) containing the synthetic gene were constructed and transformed into maize callus by particle bombardment. The constructs differed in their promoters and subcellular targeting domains. PCR analysis showed that the alpha-La transgene was integrated into the maize genome and transmitted to the F1 generation. Western blot analysis indicated that the transgenes were functional and the alpha-La protein accumulated in the target endosperm. Genomic Southern blot analysis detected no new rearrangements demonstrating stable transgene integration ...


Genetic Basis Of Autumn Growth And Winter Hardiness In Alfalfa , Baldomero Alarcón Zúñiga Jan 2003

Genetic Basis Of Autumn Growth And Winter Hardiness In Alfalfa , Baldomero Alarcón Zúñiga

Retrospective Theses and Dissertations

Alfalfa (Medicago sativa L.) has a broad geographic distribution, with populations adapted to both cold and warm climates. Despite considerable research on winter hardiness, the mechanisms and genetic control of winter survival are poorly understood. We conducted two experiments designed to identify and quantify morphological, physiological, and biochemical traits associated with winter hardiness and to estimate the genetic relationship of these traits with winter hardiness. In Experiment 1, ten alfalfa genotypes were planted in June 2000 and in May 2001 at Ames, IA, and traits were measured in August, November, and the following April in each year. Concentrations of palmitate ...


Molecular Marker Analysis Of Population Genetic Structure And Progress From Reciprocal Recurrent Selection In Two Iowa Maize (Zea Mays L) Populations , Lori Lynn Hinze Jan 2003

Molecular Marker Analysis Of Population Genetic Structure And Progress From Reciprocal Recurrent Selection In Two Iowa Maize (Zea Mays L) Populations , Lori Lynn Hinze

Retrospective Theses and Dissertations

Studies of the genetic structure of the Iowa Corn Borer Synthetic #1 (CB) and Iowa Stiff Stalk Synthetic (SS) maize populations are of particular significance because they serve as the model on which development of modern commercial hybrids are based. These populations are part of a reciprocal recurrent selection breeding strategy. With this strategy, plants from one population are crossed to plants from the opposite population (i.e. forming hybrids). These hybrids are tested, and the best ones are chosen. The plants crossed to form the chosen hybrids are identified and used to form the next generation in each population ...