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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

The Effects Of Methylamine Osmolytes On The Cytoskeleton Microtubule Assembly , Huang-Chun Tseng Jan 1999

The Effects Of Methylamine Osmolytes On The Cytoskeleton Microtubule Assembly , Huang-Chun Tseng

Retrospective Theses and Dissertations

Methylamine osmolytes are enriched in urea-rich cells. It is unclear, however, whether methylamines can promote microtubule assembly either in vitro or in cells. The osmolyte trimethylamine N-oxide (TMAO) at 200 mM increases the extent and the rate of formation of polymerized microtubule complex caused by microtubule-associated protein tau in vitro. Furthermore, TMAO counteracts the inhibition on the assembly caused by urea. Besides TMAO, betaine, another methylamine osmolyte, was found to have the same effects on MT as TMAO;Phosphorylation can affect the function of tau. Cyclic-AMP-dependent protein kinase (PKA)-phosphorylated tau does not promote tubulin assembly as judged by spectrophotometric ...


Dynamic Changes In The Localization Of Synapse Associated Proteins During Development And Differentiation Of The Mammalian Retina , Mary Heather West Greenlee Jan 1999

Dynamic Changes In The Localization Of Synapse Associated Proteins During Development And Differentiation Of The Mammalian Retina , Mary Heather West Greenlee

Retrospective Theses and Dissertations

We have examined the developmental distribution and differential localization of presynaptic terminal associated proteins in the mammalian retina. We have used antibodies specific for synaptic vesicle associated proteins Synaptotagmin, Rab 3A, Synaptophysin and Synaptobrevin, and presynaptic terminal membrane associated proteins SNAP-25 and Syntaxin, to characterize their spatio-temporal distribution during retinal differentiation and in the mature retina;The vertebrate retina has a laminar organization consisting of three cellular layers separated by two synaptic layers, the inner piexiform layer and the outer plexiform layer. In general, immunoreactivity for presynaptic terminal associated proteins was first observed in cellular layers, and as differentiation progressed ...