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Articles 1 - 6 of 6

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Molecular And Genetic Analysis Of Jil-1, A Chromatin Associated Protein Kinase Implicated In Transcriptional Regulation In Drosophila , Ye Jin Jan 1999

Molecular And Genetic Analysis Of Jil-1, A Chromatin Associated Protein Kinase Implicated In Transcriptional Regulation In Drosophila , Ye Jin

Retrospective Theses and Dissertations

Chromatin remodeling is an essential step for many chromatin-related fundamental cellular processes, such as gene replication, transcription, recombination and gene silencing. Two classes of protein complexes contribute to chromatin remodeling: protein complexes with ATPase activity and protein complexes capable of enzymatic modifications of chromosomal proteins, especially histones. Compared to well-studied histone acetylation which has been directly linked to gene transcription, the role of histone phosphorylation in chromatin remodeling for gene transcription is still poorly understood although there is some correlation between histone phosphorylation and gene transcription;A novel nuclear tandem protein kinase JIL-1 was cloned and identified in Kristen Johansen ...


Novel G-Dna Binding Proteins From The Ciliate Tetrahymena Thermophila: Purification, Characterization, Cloning And Functional Analyses , Quan Lu Jan 1999

Novel G-Dna Binding Proteins From The Ciliate Tetrahymena Thermophila: Purification, Characterization, Cloning And Functional Analyses , Quan Lu

Retrospective Theses and Dissertations

G-DNA is a family of novel four-stranded DNA structures characterized by motifs called G-quartets. Evidence is growing to suggest that G-DNA exists and plays biological roles in vivo. In order to further elucidate the functions of G-DNA, we have studied proteins that specifically bind to the DNA structure;Two G-DNA binding activities, TGPI and TGP3, were purified from the ciliate Tetrahymena thermophila. Based on the peptide sequences obtained from direct internal peptide sequencing, the cDNAs coding for the genes were cloned. Deduced protein sequences showed that TGP1 and TGP3 are novel proteins but share significant homology with each other. Furthermore ...


Fatty Acid Modification Of Cells In Culture By Overexpression Of Exogenous Desaturases , Travis Jay Knight Jan 1999

Fatty Acid Modification Of Cells In Culture By Overexpression Of Exogenous Desaturases , Travis Jay Knight

Retrospective Theses and Dissertations

COS-1 cells were transfected transiently with expression vectors encoding stearoyl-acyl-carrier-protein desaturase (SACPD) from castor bean, stearoyl-CoA desaturase (SCD) from mice, and a control vector containing SACPD cDNA in reverse orientation. The hypothesis was that both exogenous desaturases would be active in COS-1 cells, thereby increasing the proportion of monounsaturated fatty acids produced by the cells. Messenger RNA and anti-myc immunoreactive proteins were detected at 12 and 24 hours post-transfection respectively and increased through 48 hours post-transfection. When acetate was used as the fatty acid precursor in cultures for a 24-hour period starting 24 hours post-transfection, the ratio of monounsaturated to ...


The Effects Of Methylamine Osmolytes On The Cytoskeleton Microtubule Assembly , Huang-Chun Tseng Jan 1999

The Effects Of Methylamine Osmolytes On The Cytoskeleton Microtubule Assembly , Huang-Chun Tseng

Retrospective Theses and Dissertations

Methylamine osmolytes are enriched in urea-rich cells. It is unclear, however, whether methylamines can promote microtubule assembly either in vitro or in cells. The osmolyte trimethylamine N-oxide (TMAO) at 200 mM increases the extent and the rate of formation of polymerized microtubule complex caused by microtubule-associated protein tau in vitro. Furthermore, TMAO counteracts the inhibition on the assembly caused by urea. Besides TMAO, betaine, another methylamine osmolyte, was found to have the same effects on MT as TMAO;Phosphorylation can affect the function of tau. Cyclic-AMP-dependent protein kinase (PKA)-phosphorylated tau does not promote tubulin assembly as judged by spectrophotometric ...


Dynamic Changes In The Localization Of Synapse Associated Proteins During Development And Differentiation Of The Mammalian Retina , Mary Heather West Greenlee Jan 1999

Dynamic Changes In The Localization Of Synapse Associated Proteins During Development And Differentiation Of The Mammalian Retina , Mary Heather West Greenlee

Retrospective Theses and Dissertations

We have examined the developmental distribution and differential localization of presynaptic terminal associated proteins in the mammalian retina. We have used antibodies specific for synaptic vesicle associated proteins Synaptotagmin, Rab 3A, Synaptophysin and Synaptobrevin, and presynaptic terminal membrane associated proteins SNAP-25 and Syntaxin, to characterize their spatio-temporal distribution during retinal differentiation and in the mature retina;The vertebrate retina has a laminar organization consisting of three cellular layers separated by two synaptic layers, the inner piexiform layer and the outer plexiform layer. In general, immunoreactivity for presynaptic terminal associated proteins was first observed in cellular layers, and as differentiation progressed ...


Application Of Spectral Hole Burning To The Study Of In Vitro Cellular Systems , Nebojsa Milanovich Jan 1999

Application Of Spectral Hole Burning To The Study Of In Vitro Cellular Systems , Nebojsa Milanovich

Retrospective Theses and Dissertations

The high resolution spectroscopic technique of nonphotochemical hole burning (NPHB) was applied to the study of in vitro cellular systems. Suspensions of two well-characterized cell lines were used for these studies: (1) MCF-10F cells, a normal human breast epithelial cell line, and (2) MCF-7 cells, a human breast adenocarcinoma cell line. Both cell lines were stained with the dye aluminum phthalocyanine tetrasulfonate (APT) and prepared for spectroscopic study at liquid helium temperatures. Routinely measured hole burning parameters were obtained for the two cell types and compared in order to differentiate between physical properties of normal and cancer cells;A comparison ...