Open Access. Powered by Scholars. Published by Universities.®

Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 30 of 33

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Genetic And Biochemical Characterization Of Brucella Abortus Strain Rb51 , Allen Elroy Jensen Jan 1995

Genetic And Biochemical Characterization Of Brucella Abortus Strain Rb51 , Allen Elroy Jensen

Retrospective Theses and Dissertations

Brucella abortus strain RB51, a naturally derived rough variant, has been proposed to replace strain 19 for use as a vaccine against brucellosis in cattle. Utilizing fingerprinting and standard biotyping techniques, it was possible to differentiate RB51 from other brucellae and to ascertain the stability of RB51. Standardization of a genomic fingerprinting procedure using restriction endonuclease profiles of total DNA from Brucella are described. Although the focus was principally on B. abortus isolates from cattle, the investigation included field isolates from bison and elk, reference strains, vaccinal strains, and genetically-engineered brucellae;Pulsed-field gel electrophoresis profiles of the reference type strains ...


Characterization Of A Rat Clonal [Beta]-Cell Line, Rinm5f, In A Passage- And Morphology-Dependent Manner , Bum-Sup Lee Jan 1995

Characterization Of A Rat Clonal [Beta]-Cell Line, Rinm5f, In A Passage- And Morphology-Dependent Manner , Bum-Sup Lee

Retrospective Theses and Dissertations

A novel insulin-like immunoreactivity, adrenergic receptors (ARs) and voltage-dependent Ca[superscript]2+ channels (VDCCs) were characterized in a clonal [beta]-cell line, RINm5F, in a passage- and morphology-dependent manner. The cells exhibited two morphological features; the differentiated cells (DCs) with long neuron-like processes and the undifferentiated cells (UCs) without the processes;As morphology of the cells changed from DCs to UCs, amount of secreted insulin gradually decreased; however, insulin reacted to antiserum vs. bovine insulin (ABI) gradually increased from barely detectable amounts. Immunoblotting and immunocytochemistry showed that antiserum vs. rat insulin reacted with insulin from both cell types. ABI did ...


Expression And Characterization Of Bovine Rotavirus Proteins In The Baculovirus System , Young Soo Lyoo Jan 1995

Expression And Characterization Of Bovine Rotavirus Proteins In The Baculovirus System , Young Soo Lyoo

Retrospective Theses and Dissertations

Recombinant proteins of bovine rotavirus VP2, VP4, VP6 and VP7 were expressed in insect cells using baculovirus expression vectors. Expressed recombinant proteins were similar to those of rotavirus. Recombinant viral antigens expressed in insect cells reacted with polyclonal antiserum to the VMRI strain of BRV by an indirect immunofluorescence test. Expressed recombinant proteins were characterized by radioimmunoprecipitation and their molecular mass was found to be similar to that of in wild type rotavirus. These four recombinant proteins were antigenic in guinea pigs and induced antibodies to the corresponding rotavirus proteins as detected by indirect immunofluorescence test. Recombinant viruses Bac.pVL ...


Calsensin, A Novel Calcium-Binding Protein Expressed In A Subset Of Peripheral Leech Neurons Fasciculating In A Single Axon Tract , Kristen Kay Briggs Jan 1995

Calsensin, A Novel Calcium-Binding Protein Expressed In A Subset Of Peripheral Leech Neurons Fasciculating In A Single Axon Tract , Kristen Kay Briggs

Retrospective Theses and Dissertations

The lan 3-6 monoclonal antibody recognizes a subpopulation of peripheral neurons whose afferents segregate into a single fascicle within the developing leech central nervous system. The lan 3-6 monoclonal antibody was used to isolate a clone encoding a novel protein, calsensin, which contains two EF-hand calcium-binding domains. In situ hybridizations with calsensin riboprobes, Western blots of lan 3-6 monoclonal antibody immunoprecipitations, and immunohistochemistry with polyclonal antibodies generated against a partially-purified calsensin fusion protein confirmed that the sequence encoding the lan 3-6 antigen has been identified. Partially-purified calsensin fusion protein binds [superscript]45Ca[superscript]2+ in vitro, suggesting that the calsensin ...


Effects Of Ear Developmental Temperature On Fine Structure Of Maize Starch , Ting-Jang Lu Jan 1995

Effects Of Ear Developmental Temperature On Fine Structure Of Maize Starch , Ting-Jang Lu

Retrospective Theses and Dissertations

Two genetically unrelated normal dent maize inbreds, ICI63 and ICI92, with different heterotic backgrounds were used for studying environmental temperature effects on grain yield and quality. These two maize inbreds are used in commercially available hybrids and are proprietary to ICI Seeds. The maize inbreds were grown in a greenhouse to control the growing condition of the plants. After 14 days post-pollination, the ears were wrapped in temperature control devices set at 25 or 35∘ C during the grain-filling period. Grain yield, kernel weight, and kernel density were less for ears developing at 35∘ C than for those at 25 ...


Molecular And Biochemical Investigation Of The Mechanisms Of Acclimation To Chilling Stress In Maize Seedlings , Marc David Anderson Jan 1995

Molecular And Biochemical Investigation Of The Mechanisms Of Acclimation To Chilling Stress In Maize Seedlings , Marc David Anderson

Retrospective Theses and Dissertations

Seedlings of chilling-sensitive maize inbred G50 (Pioneer) survived poorly when exposed to a chilling stress. Acclimation or ABA treatment caused a dramatic improvement of chilling tolerance. Differences in gene expression between acclimated and unacclimated seedlings were investigated and three cDNA clones (designated car333, car30, and car757) were isolated, representing transcripts that were up-regulated during acclimation. Car30 and car757 were not homologous to any known sequence but car333 was 97.8% homologous to cat3, maize mitochondrial catalase. The up-regulation of CAT3 during acclimation suggested that chilling stress caused the generation of H[subscript]2O[subscript]2 in the mitochondria and increased ...


Compilation And Analysis Of Mycobacterium Paratuberculosis Promoters , John Patrick Bannantine Jan 1995

Compilation And Analysis Of Mycobacterium Paratuberculosis Promoters , John Patrick Bannantine

Retrospective Theses and Dissertations

Mycobacterium paratuberculosis transcriptional and translational signals were studied to gain insight into gene expression in this organism. To study M. paratuberculosis promoter structures, a more versatile promoter selection vector was constructed from the pKO1 parent vector. This new plasmid, pJJ2, was used to identify 11 promoter fragments from an M. paratuberculosis DNA library. In addition, a previously characterized M. paratuberculosis promoter, contained in a 493-bp EcoRI fragment, was cloned into the new vector to test the efficacy in cloning novel M. paratuberculosis promoters. In a related study, an expression probe shuttle plasmid (pYUB76) was employed to clone M. paratuberculosis expression ...


Biochemical And Molecular Biological Characterization Of Plant Acetyl-Coa Carboxylases , James J. Caffrey Jan 1995

Biochemical And Molecular Biological Characterization Of Plant Acetyl-Coa Carboxylases , James J. Caffrey

Retrospective Theses and Dissertations

Acetyl-CoA carboxylase is a biotin-containing enzyme which catalyzes the ATP-dependent carboxylation of acetyl-CoA to form malonyl-CoA. Plants are unique among organisms in that malonyl-CoA is utilized in both de novo fatty acid biosynthesis, and as an intermediate in a number of other plant primary and secondary metabolic pathways. Some of these metabolic pathways operate in different subcellular and cellular compartments. Therefore, plants have a mechanism to generate separate pools of malonyl-CoA. Understanding this mechanism has been the impetus for the research reported in this dissertation;Immunological and structural analyses of a 60 kDa biotinylated polypeptide (60kDBP) from carrot indicate that ...


Biosynthetic Pathways In Oxalobacter Formigenes , Nancy A. Cornick Jan 1995

Biosynthetic Pathways In Oxalobacter Formigenes , Nancy A. Cornick

Retrospective Theses and Dissertations

Oxalate is the only substrate that supports the growth of the gram negative anaerobe, Oxalobacter formigenes. Oxalate is decarboxylated to formate plus CO[subscript]2. A small amount of acetate (0.5-1 mM) is required for biosynthetic reactions. Oxalate is reduced and assimilated into cell biomass by aerobic oxalate-degrading bacteria using either the glycerate pathway or the serine pathway. Oxalate is reduced to 3P-glycerate and assimilated as a C[subscript]3 unit. We detected the enzymatic activities of glycerate pathway but not those of the serine pathway in cell-free extracts of O. formigenes;Four potential sources of carbon for cell ...


Rflp Mapping Of Quantitative Trait Loci For Reproductive, Morphological, And Seed Traits Of Soybean (Glycine Max L.) Across Environments , Heike Kross Jan 1995

Rflp Mapping Of Quantitative Trait Loci For Reproductive, Morphological, And Seed Traits Of Soybean (Glycine Max L.) Across Environments , Heike Kross

Retrospective Theses and Dissertations

Quantitative trait loci (QTL) and QTL x environment interaction were identified for 11 agronomic traits in soybean, when measured at two locations in each of two years in Iowa. The segregating population consisted of 76 F[subscript]2:5/F[subscript]2:6 families derived from the cross between the two soybean (Glycine max (L.) Merr.) cultivars 'Minsoy' (PI27.890) and 'Noir 1' (PI 290.136). A genetic linkage map consisting of 81 markers in 25 linkage groups was used to locate QTL by interval mapping. Analyses of variance was performed on 72 linked and unlinked markers to test for ...


Spectral Hole Burning Studies Of Photosystem Ii , Hai-Chou Chang Jan 1995

Spectral Hole Burning Studies Of Photosystem Ii , Hai-Chou Chang

Retrospective Theses and Dissertations

Low temperature absorption and hole burning spectroscopies were applied to the D1-D2-cyt b[subscript]559, and the CP47 and CP43 antenna protein complexes of Photosystem II from higher plants. Low temperature transient and persistent hole-burning data and theoretical calculations on the kinetics and temperature dependence of the P680 hole profile are presented and provide convincing support for the linker model. Implicit in the linker model is that the 684-nm-absorbing Chl a serve to shuttle energy from the proximal antenna complex to reaction center. The stoichiometry of isolated Photosystem II Reaction Center (PSII RC) in several different preparations is also discussed ...


Cloning And Characterization Of Two Chlorophyll Synthesis Genes Expressed During Tomato Fruit Development , Gary Francis Polking Jan 1995

Cloning And Characterization Of Two Chlorophyll Synthesis Genes Expressed During Tomato Fruit Development , Gary Francis Polking

Retrospective Theses and Dissertations

Full-length clones that encode tomato (Lycopersicon esculentum Mill.) glutamate-1-semialdehyde-2,1-aminomutase (GSAAM) and 5-aminolevulinic acid dehydratase (ALAD) were isolated and characterized. Sequence analysis showed that tomato GSAAM and ALAD clones exhibited a high level of homology to corresponding clones found in other plant species. The primary structure of GSAAM predicted a 481-amino acid precursor that comprised a 46.7 kDa, 437-amino acid mature protein and a transit peptide of 44 amino acids. The primary structure of ALAD predicted a 430-amino acid precursor that comprised a 41.7 kDa, 383-amino acid mature protein and a 47-amino acid transit sequence. Southern analysis showed ...


Toxicity, Metabolism And Applied Uses Of 3,4-Didehydroretinol , Pamela Kay Duitsman Jan 1995

Toxicity, Metabolism And Applied Uses Of 3,4-Didehydroretinol , Pamela Kay Duitsman

Retrospective Theses and Dissertations

The comparative potencies of the all-trans isomers of 3,4-didehydroretinyl acetate (A2), retinyl acetate (A1), and retinoic acid (RA) to induce terata, embryolethality, and behavioral disruptions in offspring of timed-pregnant Sprague-Dawley rats was evaluated. Single equimolar doses (1-352 [mu]mol/kg BW) of the retinoids were given orally in corn oil on day 8.5 of gestation. All retinoids tested were teratogenic and embryolethal at dose ranges of 35-352 [mu]mol/kg BW. Embryolethality also occurred with both A2 and RA at 11 [mu]mol/kg BW. Biochemical profiles of both maternal and fetal tissue indicated that increased teratogenicity and ...


Study Of The Structural Requirements Of E Coli Valine Transfer Rna In Aminoacylation And Protein Synthesis , Mingsong Liu Jan 1995

Study Of The Structural Requirements Of E Coli Valine Transfer Rna In Aminoacylation And Protein Synthesis , Mingsong Liu

Retrospective Theses and Dissertations

The structural requirements for the function of the 3[superscript]' CCA end of E. coli valine transfer RNA in aminoacylation and protein synthesis have been investigated, by studying the activities of 3[superscript]' end tRNA[superscript] Val variants in these processes. These studies show that 3[superscript]' end tRNA[superscript] Val variants can be obtained which are functional in both aminoacylation and later steps of protein synthesis. Replacement of the 3[superscript]' terminal adenosine with either cytosine or uracil yields tRNAs that retain almost full aminoacylation activity (40-50% that of wild type tRNA[superscript] Val). The tRNA[superscript] Val variant ...


The Relationship Between The Calpain Enzyme System And The Postmortem Degradation Of Selected Myofibrillar Proteins , Elisabeth Jane Huff Lonergan Jan 1995

The Relationship Between The Calpain Enzyme System And The Postmortem Degradation Of Selected Myofibrillar Proteins , Elisabeth Jane Huff Lonergan

Retrospective Theses and Dissertations

The protease [mu]-calpain degrades the myofibrillar proteins titin, nebulin, filamin, desmin and troponin-T. Titin and nebulin have been reported to be affected by sample preparation. This study showed that there was little difference in the rate of degradation of the intact forms due to sample preparation (whole muscle preparations vs. purified myofibrils). The postmortem degradation of titin, nebulin, filamin, desmin, and troponin-T was monitored using SDS-PAGE and Western blotting techniques at 0, 1, 3, 7, 14, 28, and 56 days postmortem. Postmortem aged samples with lower shear force values exhibited faster degradation of the five myofibrillar proteins. Similar degradation ...


Sequence Variation Characteristics Of D-Loop, Rrna, Trna, And Polypeptide Coding Region Of Bovine Mitochondrial Dna , Jianming Wu Jan 1995

Sequence Variation Characteristics Of D-Loop, Rrna, Trna, And Polypeptide Coding Region Of Bovine Mitochondrial Dna , Jianming Wu

Retrospective Theses and Dissertations

By cloning and sequencing the mtDNA of cows from different maternal lineages, it was found that multiple heteroplasmic mtDNA states within the D-loop and rRNA genes of mtDNA existed extensively in Holstein cattle. Meanwhile, the nature and transmission of mtDNA were investigated. The mtDNA population in a specific animal was found to be a mixture of different genotypes. Because heteroplasmy was observed frequently and seemingly is persistent, selected amplification of specific mtDNA molecules may not be a part of mtDNA inheritance. At same time, four hypervariable sites were located at nt 169, 216, and 1594 between nt 352 to 364 ...


Aspects Of Protein Phosphatase Regulation , Jennifer Marie Imparl Radosevich Jan 1995

Aspects Of Protein Phosphatase Regulation , Jennifer Marie Imparl Radosevich

Retrospective Theses and Dissertations

Aspects of regulation of two protein phosphatases involved in signal tranduction are described in this dissertation. The first phosphatase, calcineurin, is involved in T-cell activation and is also regulated by Ca2+/calmodulin. Because most studies on the interaction between calmodulin and the enzymes which it activates have focused on the role of hydrophobic residues in the interaction, we have instead focused on the role of the positively charged arginine residue in calcineurin-calmodulin interaction. To this end, certain arginine residues in the C-terminal portion of calcineurin were converted to the neutral amino acid citrulline by the action of peptidylarginine deiminase. The ...


Characterization And Application Of Porcine Liver Aldehyde Oxidase In The Removal Of Off-Flavors From Soy Proteins , Peeyush Maheshwari Jan 1995

Characterization And Application Of Porcine Liver Aldehyde Oxidase In The Removal Of Off-Flavors From Soy Proteins , Peeyush Maheshwari

Retrospective Theses and Dissertations

Two enzyme forms (PAO-I and PAO-II) of porcine liver aldehyde oxidase (aldehyde: oxygen oxidoreductase, EC 1.2.3.1) were purified to homogeneity using affinity chromatography. The heat treatment and ammonium sulfate fractionation steps resulted only in a partial purification. Both enzyme forms, PAO-I and PAO-II, have similar pIs of 5.8 and molecular weight of 262,000 and 255,000 daltons, respectively;The two enzyme forms exhibited different substrate specificities. Compared to PAO-II, PAO-I showed higher affinity for the medium-chain aldehydes (pentanal and hexanal) which are mainly responsible for the greeny and beany off-flavor of soybean products. The PAO-I ...


Restriction Fragment Length Polymorphism In Sorghum (Sorghum Bicolor (L.) Moench): Characterization Of Genetic Diversity And Quantitative Trait Loci , Dario Ahnert Jan 1995

Restriction Fragment Length Polymorphism In Sorghum (Sorghum Bicolor (L.) Moench): Characterization Of Genetic Diversity And Quantitative Trait Loci , Dario Ahnert

Retrospective Theses and Dissertations

Restriction fragment length polymorphisms (RFLPs) were employed to investigate genetic diversity and quantitative trait loci (QTL) for morphological characters on sorghum (Sorghum bicolor (L.) Moench). The objectives of this research were accomplished by studying two different sets of genotypes. One set was composed of 105 elite inbred lines (47 B- and 58 R-lines) and the other of 152 F[subscript]2:3 lines. In the R- and B-lines set, the objectives were to define the level of genetic similarity (GS) and relationships within these lines. In the 152 F[subscript]2:3 lines set, the objectives were to characterize QTL ...


Line Narrowing Spectroscopic Studies Of Dna-Carcinogen Adducts And Dna-Dye Complexes , Myungkoo Suh Jan 1995

Line Narrowing Spectroscopic Studies Of Dna-Carcinogen Adducts And Dna-Dye Complexes , Myungkoo Suh

Retrospective Theses and Dissertations

Laser-induced fluorescence line narrowing (FLN) and non-line narrowing (NLN) spectroscopic methods were applied to conformational studies of stable DNA adducts of the 7[beta],8[alpha]-dihydoxy-9[alpha],10[alpha]-epoxy-7,8,9, 10-tetrahydrobenzo (a) pyrene (anti-BPDE). Stereochemically distinct (+)-trans-, (-)-trans-, (+)-cis- and (-)-cis adducts of anti-BPDE bound to exocyclic amino group of the central guanine in the oligonucleotide, 5'-d(CCATCGCTACC) · (GGTAGCGATGG), exist in a mixture of conformations in frozen aqueous buffer matrices. The (+)-trans adduct adopts primarily an external conformation with a smaller fraction (~25%) exists in a partially base-stacked conformation. Both cis adducts were found to be ...


The Interaction Between Actin Filaments And The Cytoskeleton-Membrane Attachment Site Protein Talin , Jinwen Zhang Jan 1995

The Interaction Between Actin Filaments And The Cytoskeleton-Membrane Attachment Site Protein Talin , Jinwen Zhang

Retrospective Theses and Dissertations

Talin, a protein localized primarily in cell-matrix type adherens junctions, is involved in linking the cytoskeleton to the cell membrane. The major objectives of my study were to (1) determine the structural basis for the effect of pH and ionic strength on the direct talin-actin interaction, (2) examine the effects of other factors, such as specific phospholipids, on the talin-actin interaction, and (3) compare abilities of human platelet and avian smooth muscle talins to interact with actin. Negative staining results showed that at pH 6.4 and low ionic strength, talin extensively crosslinked actin filaments into both tight networks and ...


Molecular Basis Of Resistance To Soybean Mosaic Virus: Reverse Transcription (Rt)-Pcr And Strain Complementation Analysis In Soybeans , Michael E. Omunyin Jan 1995

Molecular Basis Of Resistance To Soybean Mosaic Virus: Reverse Transcription (Rt)-Pcr And Strain Complementation Analysis In Soybeans , Michael E. Omunyin

Retrospective Theses and Dissertations

Soybean (Glycine max(L.) Merr.) is grown in both developed and developing countries primarily for vegetable oil and protein products. It is the third most widely planted crop in the United States. Soybean mosaic virus (SMV) can affect soybean production by causing reduction in grain yield and quality. The virus is a member of the large and economically important family Potyviridae and several strains have been identified. Soybean lines exist that contain single genes (Rsv) for resistance to SMV. Therefore, SMV and soybean have been used as a model to understand plant resistance to virus disease. One facet of this ...


Molecular Analyses Of Group 1 Late Embryogenic Abundant (Lea) Genes In Soybean (Glycine Max (L.) Merr.) , Éberson Sanches Calvo Jan 1995

Molecular Analyses Of Group 1 Late Embryogenic Abundant (Lea) Genes In Soybean (Glycine Max (L.) Merr.) , Éberson Sanches Calvo

Retrospective Theses and Dissertations

The entire soybean Sle gene family was cloned and characterized. The five Sle genes (Sle1-5) could be divided into two classes based on sequence identity. Sle1-4 map to 4 different linkage groups. Sle1 and Sle5 are expressed weeks earlier in seed development than Sle2 and Sle3. Sle4 is a pseudogene. Maximal levels of Sle mRNA accumulate in the seeds, before desiccation had occurred, and decline rapidly upon seed imbibition. Desiccation does not induce Sle expression in seeds. Unlike most of its homologs, ABA is not necessary to induce Sle expression in cultured embryos and only moderately increases Sle mRNA levels ...


Identification And Characterization Of A Chicken Major Histocompatibility Complex Class Ii[Beta] Gene Promoter , Yunfei Chen Jan 1995

Identification And Characterization Of A Chicken Major Histocompatibility Complex Class Ii[Beta] Gene Promoter , Yunfei Chen

Retrospective Theses and Dissertations

The major histocompatibility complex (MHC) contains a variety of genes. Among them, the class II genes encode proteins involved in antigen presentation to helper T cells, a key step in initiating immune responses. The regulation of class II gene expression has been extensively studied in mammals. Such studies have been lacking in the avian species, despite the well-established association of the avian MHC with disease resistance and production traits. The objective of this study was to functionally analyze a putative chicken MHC class II gene promoter;Using the chloramphenicol acetyltransferase (CAT) reporter system, a functional chicken MHC class II[beta ...


Protein-Binding Sequences That May Regulate Organ-Preferential Transcription Of Zrp2, A Zea Mays Root-Preferential Gene, Therese Shamala Tirimanne Jan 1995

Protein-Binding Sequences That May Regulate Organ-Preferential Transcription Of Zrp2, A Zea Mays Root-Preferential Gene, Therese Shamala Tirimanne

Retrospective Theses and Dissertations

In this study, DNA-protein interactions of the first kilobase region proximal to the transcription start site of the zrp2 (Zea mays root-preferential 2) gene promoter was analyzed. The intent of this research was to identify regions of the promoter and DNA-protein interactions, that are responsible for root-preferential transcription. In vitro run-on transcription assays had revealed dramatically higher levels of transcription in 3-d-old roots than in 5-d-old leaves (Held, 1993). Interactions between regions of the zrp2 promoter and nuclear protein extracts from 3-d-old roots and 5-d-old leaves of maize were analyzed by gel mobility-shift assays. Two DNA-protein complexes with each protein ...


The Role(S) Of Arginine-Specific Mono(Adp-Ribosyl)Transferase In Skeletal Muscle Cells , Hui-Yu Huang Jan 1995

The Role(S) Of Arginine-Specific Mono(Adp-Ribosyl)Transferase In Skeletal Muscle Cells , Hui-Yu Huang

Retrospective Theses and Dissertations

The overall goal of this research project was to examine the biological function of arginine-specific mono(ADP-ribosyl) transferase in skeletal muscle cells. To better understand the function of this enzyme in skeletal muscle, the experimental approach to accomplish this goal consisted of two specific objectives. The first objective was to identify the protein substrates for this enzyme in myogenic cell cultures and cellular fractions. Embryonic chick primary muscle cell cultures were used as a working system for this purpose. In membrane fractions of 96-h myotube cultures incubated with [[superscript]32 P]NAD under conditions selected for mono(ADP-ribosyl)ation, proteins ...


Elm1p, A Protein Kinase That Regulates Cell Morphology In Saccharomyces Cerevisiae , Carla Marie Koehler Jan 1995

Elm1p, A Protein Kinase That Regulates Cell Morphology In Saccharomyces Cerevisiae , Carla Marie Koehler

Retrospective Theses and Dissertations

Saccharomyces cerevisiae is a pleimorphic ascomycete, capable of growing in the yeast form or the pseudohyphal form. The pseudohyphal form is characterized by elongated cells that possess a unipolar budding pattern and grow in chains away from the center of the colony. This change in morphology is induced by nitrogen starvation in the presence of carbon. A collection of 14 elm (elongated morphology) mutants previously have been isolated that grow as pseudohyphae constitutively. Of these mutants, the product of the ELM1 gene was identified and coded for a novel protein kinase. From genetic analyses, ELM1 seemingly functioned downstream of STE7 ...


Characterization Of Genes Expressed During Vegetative Organ Development In Potato (Solanum Tuberosum L.) , Sang-Gu Kang Jan 1995

Characterization Of Genes Expressed During Vegetative Organ Development In Potato (Solanum Tuberosum L.) , Sang-Gu Kang

Retrospective Theses and Dissertations

The cp rps16 cDNA encoding the chloroplast ribosomal protein S16 and a MADS-box gene cDNA, POTM1-1, were isolated and characterized in potato (Solanum tuberosum L.) to understand molecular mechanisms of plant vegetative organ development;The rps16 gene is located between the trnK gene for tRNA Lys(UUU) and trnQ gene for tRNA Gln (UUG) on the same strand in the chloroplast genome. The ribosomal binding site, 5'-AGGA-3', is located at nucleotide position -6 to -9 from the initiation codon ATG. The coding sequence contains an 88 amino acid open reading frame (ORF) that is interrupted by an 855 bp ...


Investigation Of The Gtp-Binding Consensus Sequences In Escherichia Coli Adenylosuccinate Synthetase And The Enzyme's Reaction Mechanism , Chulhun Kang Jan 1995

Investigation Of The Gtp-Binding Consensus Sequences In Escherichia Coli Adenylosuccinate Synthetase And The Enzyme's Reaction Mechanism , Chulhun Kang

Retrospective Theses and Dissertations

In this dissertation, on the basis of the similarities in sequence and structure between GTP-binding proteins and AMPSase, it was of interest to determine whether or not the GTP-binding consensus sequences have similar functions. It will be shown that the GTP-binding consensus sequences found in AMPSase play similar roles to those of the GTPase superfamily. The kinetic results show that Lys331 interacts with GTP through hydrophobic interactions between its linear side chain and the aromatic ring of the guanine base of GTP. Also, structural characterization of the G15V mutant using circular dichroism (CD) spectrometry, NMR spectroscopy, and spectrofluorometry indicated that ...


Characterization And Application Of A Novel Cholesterol-Reducing Anaerobe, Eubacterium Coprostanoligenes Atcc 51222 , Ling Li Jan 1995

Characterization And Application Of A Novel Cholesterol-Reducing Anaerobe, Eubacterium Coprostanoligenes Atcc 51222 , Ling Li

Retrospective Theses and Dissertations

A novel cholesterol-reducing bacterium, Eubacterium coprostanoligenes ATCC 51222, was characterized and tested for its hypocholesterolemic effects in animal models. The bacterium did not require cholesterol for growth and synthesized cholesterol reductase constitutively. Lecithin was required for growth but excess lecithin in media did not improve bacterial growth and coprostanol production. Addition of pyruvate, lactose, and most reducing agents in media increased bacterial growth and coprostanol production. The bacterium was sensitive to most antibiotics but had strong resistance to tetracycline. A resting cell assay was established to evaluate the cholesterol reductase activity. E. coprostanoligenes reduced cholesterol through an indirect pathway. Intermediates ...