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Biochemistry, Biophysics, and Structural Biology Commons

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Articles 1 - 5 of 5

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Purification, Characterization And Cloning Of A Periplasmic Catalase From B Abortus And The Role It Plays In The Pathogenesis Of Brucella , Zheng Yu Sha Jan 1994

Purification, Characterization And Cloning Of A Periplasmic Catalase From B Abortus And The Role It Plays In The Pathogenesis Of Brucella , Zheng Yu Sha

Retrospective Theses and Dissertations

A periplasmic catalase has been identified and purified from Brucella abortus using a procedure which fractionates periplasmic B. abortus proteins from other cellular components while retaining biological activity. The biochemical characteristics of the purified enzyme were studied and the gene encoding the enzyme cloned and sequenced. SDS polyacrylamide gel electrophoresis of the purified enzyme revealed only a single subunit with a molecular weight of 59,000. Gel filtration indicates the native protein is a tetramer. The native protein has a visible absorption spectrum with maxima at 407, 501, and 628 nm which is typical of mammalian, but not some bacterial ...


Characterization And Immunogenicity Of The Heat Shock Protein, Hsp60 Of Brucella Abortus , Dennis Gor Jan 1994

Characterization And Immunogenicity Of The Heat Shock Protein, Hsp60 Of Brucella Abortus , Dennis Gor

Retrospective Theses and Dissertations

Brucella abortus is the causative agent of bovine brucellosis, a disease that causes spontaneous abortion in cows. The most effective form of protection against this pathogen is immunization; however, Brucella abortus strain 19 (the standard vaccine against Brucella abortus) is not completely protective. The vaccination of laboratory mice with the heat shock protein, hsp60 derived from Brucella abortus, using live attenuated recombinant Salmonella typhimurium expressing this protein (hsp60) as a delivery system, and the characterization of the immune response to Brucella abortus hsp60 over a 12 week time course following vaccination is described;Infection of BALB/c strain of laboratory ...


G-Wires: The Growth And Characterization Of A G4-Dna Nanostructure , Thomas Cosgrove Marsh Jan 1994

G-Wires: The Growth And Characterization Of A G4-Dna Nanostructure , Thomas Cosgrove Marsh

Retrospective Theses and Dissertations

Guanine-rich sequences are vital components in the genomes of many organisms. For example, G-rich sequences are found in telomeres, fragile X locus, promoters, IgG switch regions, recombinational hot spots and the HIV RNA dimerization domain. The functions of these G-rich sequences rely in part on guanine self-recognition. G-rich sequences can adopt a quadruple helical conformation in the presence of specific monovalent and divalent metal cations which are also required for maintaining the quadruplex stability. The structural basis of the quadruplex is a cyclic Hoogsteen hydrogen bonded guanine tetrad known as the G-quartet. Sequences capable of forming G-quartets are classified as ...


Telomeric Nucleic Acids: C-Strand Structure And A Telomerase Rna Mutant , Shawn Cameron Ahmed Jan 1994

Telomeric Nucleic Acids: C-Strand Structure And A Telomerase Rna Mutant , Shawn Cameron Ahmed

Retrospective Theses and Dissertations

Telomeres, the ends of linear chromosomes, are composed of simple tandem repeats which are usually G·C rich. Telomeres are essential for chromosome stability, organizing the nuclear architecture and ensuring complete replication of the chromosomal terminus. To understand how telomeres carry out these fundamental cellular roles, one must understand the structural and dynamic properties of telomeric repeat sequences. Structural and genetic approaches were taken to learn more about telomeric nucleic acids;The structural portion of my research concerned an unusual DNA structure formed by the C-rich strand of telomeric DNA. Telomeric C-strand sequences form non-Watson-Crick structures in supercoiled plasmids at ...


Soybean Cultivar Development: A Genome Perspective , Lisa Lou Lorenzen Jan 1994

Soybean Cultivar Development: A Genome Perspective , Lisa Lou Lorenzen

Retrospective Theses and Dissertations

The purpose of this dissertation was to retrospectively analyze the process of soybean cultivar development from a molecular perspective, by following the inheritance of RFLP markers in a pedigree analysis. Five methods of analysis are included in this dissertation;Sixty-four plant introductions, cultivars, and breeding lines were analyzed to define a core set of 97 probes with a high likelihood of detecting a polymorphism in elite germplasm. The ability to trace regions of chromosomes from parent to offspring and the application of pedigree-based mapping was demonstrated, which is the cosegregation of phenotypic traits and RFLP markers based on pedigree information ...