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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Molecular Mechanisms Of Mycoplasma Hyopneumoniae Adherence To Swine Respiratory Epithelial Cells, Qijing Zhang Jan 1994

Molecular Mechanisms Of Mycoplasma Hyopneumoniae Adherence To Swine Respiratory Epithelial Cells, Qijing Zhang

Retrospective Theses and Dissertations

A microtiter plate adherence assay for Mycoplasma hyopneumoniae was established by using purified swine tracheal cilia which are the natural targets for the mycoplasma. M. hyopneumoniae bound specifically to solubilized cilia immobilized onto microtiter plates. Dextran sulfate, heparin, chondroitin sulfate, laminin, mucin, and fucoidan significantly inhibited binding of the mycoplasmas to cilia. Heparin, mucin, fucoidan, and chondroitin sulfate interacted with the adhesins on the surface of mycoplasmas, whereas laminin blocked the receptors in cilia. Treatment of cilia with neuraminidase appeared to promote adherence of the mycoplasmas; whereas, treatment of cilia with sodium metaperiodate decreased the binding. In the second study ...


Molecular Characterization Of Porcine Respiratory Coronavirus Isolates With Varying Pathogenicity , Eric Martin Vaughn Jan 1994

Molecular Characterization Of Porcine Respiratory Coronavirus Isolates With Varying Pathogenicity , Eric Martin Vaughn

Retrospective Theses and Dissertations

Three new isolates of porcine respiratory coronavirus (PRCV) were isolated and characterized. Two of the PRCV isolates, AR310 and LEPP, were shown to produce moderate interstitial pneumonia, whereas the third PRCV isolate, IA1894, was only mildly pathogenic in five-week-old specific pathogen free pigs. The previously described PRCV isolate ISU-1, a mildly pathogenic PRCV isolate, was also included in this study. These PRCV isolates with varying pathogenicity provided an opportunity to determine if there are certain genes that correlate with virulence, as the genes 3 and 3-1 have been hypothesized to be virulence determinants for transmissible gastroenteritis virus (TGEV) isolates. Using ...