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Biochemistry, Biophysics, and Structural Biology Commons

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Retrospective Theses and Dissertations

1994

Cellular

Cell and Developmental Biology

Articles 1 - 3 of 3

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Identification Of Potential Oat Phytochrome A Mrna Degradation Mechanisms , David Charles Higgs Jan 1994

Identification Of Potential Oat Phytochrome A Mrna Degradation Mechanisms , David Charles Higgs

Retrospective Theses and Dissertations

The phytochrome plant photoreceptor is important for perceiving the light environment and initiating plant developmental and environmental responses. When dark-grown oat seedlings are treated with light the phytochrome A (PHYA) mRNA abundance rapidly declines due, in part, to the inherent instability of the PHYA mRNA. Messenger RNA stability is an important process in regulating eukaryotic gene expression. My primary goal was to investigate both the mechanism of oat PHYA mRNA degradation and cis-acting sequences involved in regulating oat PHYA mRNA degradation. An oat protoplast system was established to investigate its use as a quick and reliable system to study plant ...


Regulation Of Mitogen Regulated Protein/Proliferin Gene Expression In 3t3 Cells By Basic Fibroblast Growth Factor , Manzoor Ali P. K. Mohideen Jan 1994

Regulation Of Mitogen Regulated Protein/Proliferin Gene Expression In 3t3 Cells By Basic Fibroblast Growth Factor , Manzoor Ali P. K. Mohideen

Retrospective Theses and Dissertations

Mitogen regulated protein/proliferin (MRP/PLF) is a murine uterine growth factor belonging to the prolactin/growth hormone gene superfamily. Basic fibroblast growth factor (bFGF) regulates its expression in 3T3 cells and is speculated to be among its regulators in vivo. In order to gain insights into its regulation, experiments were performed to elucidate the mechanisms by which bFGF regulates mrp/plf gene expression in 3T3 cells. RT-PCR analyses demonstrated that of the three different forms of MRP/PLF proteins only one, PLF1, is expressed in these cells in response to bFGF. PLF1 could be the product of either plf42 ...


Genetic Analysis Of Pseudohyphal Growth In The Budding Yeast Saccharomyces Cerevisiae , Melissa Jo Blacketer Jan 1994

Genetic Analysis Of Pseudohyphal Growth In The Budding Yeast Saccharomyces Cerevisiae , Melissa Jo Blacketer

Retrospective Theses and Dissertations

The budding yeast Saccharomyces cerevisiae is a dimorphic organism that can assume either a yeast-like or pseudohyphal form. Nitrogen limitation induces pseudohyphal growth, which is characterized by branched chains of elongated cells. Pseudohyphal cells can grow invasively in agar medium, whereas yeast-like cells do not. To identify factors involved in morphologic differentiation, S. cerevisiae mutants exhibiting a constitutive cell elongation morphology were isolated. Genetic analysis identified 28 recessive and 2 semi-dominant mutations that cause abnormal morphology, and placed these in 14 distinct gene loci, termed ELM, for ELongated Morphology. Many elm mutations cause multiple aspects of pseudohyphal growth, and thus ...