Articles 1 - 1 of 1
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Oat Phya Containing Transgenic Tobacco: An Examination Of Oat Phya Mrna Stability And The Role Of Oat Phya In Germination Of Tobacco Seed , Linda Joanne Barnes
Retrospective Theses and Dissertations
The regulation of oat phytochrome A (phyA) mRNA stability and the photoregulation of germination of tobacco seeds containing the oat phyA gene was examined using oat phyA containing transgenic tobacco. Oat phyA mRNA has been previously shown to be a relatively unstable mRNA. The intent of this research was to identify elements within the phyA mRNA responsible for rendering the message unstable. The approach taken was to use tobacco transformed with either the full-length or truncated versions of the oat phyA gene and examine the half-lives of the resulting oat phyA mRNAs in transgenic tobacco. RNA blot analysis of oat ...