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Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Retrospective Theses and Dissertations

1994

Botany (Physiology and molecular biology)

Discipline

Articles 1 - 2 of 2

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Expression Of Chlamydomonas Reinhardtii Periplasmic Carbonic Anhydrase In Escherichia Coli And Transgenic Tobacco Plants , Cyril Sebastian Roberts Jan 1994

Expression Of Chlamydomonas Reinhardtii Periplasmic Carbonic Anhydrase In Escherichia Coli And Transgenic Tobacco Plants , Cyril Sebastian Roberts

Retrospective Theses and Dissertations

Carbonic anhydrase (CA) catalyzes the reversible hydration of CO2. The periplasmic CA gene CAH1 of Chlamydomonas reinhardtii codes for a highly processed secreted glycoprotein. The primary translation product of the CAH1 gene is targeted to the algae's ER, where it is proteolytically processed to yield two different subunits, glycosylated, assembled into an active heterotetramer, and secreted. Expression of CAH1 in Escherichia coli and tobacco plants were effected in this study. For bacterial expression, two constructs, one with and another without, the algal target leader sequence were used. Similarly, after replacing the algal's target leader sequence with that from ...


Molecular And Ultrastructural Aspects Of The Adaptation Of Chlamydomonas Reinhardtii To Limiting Co2 , Anne Margaret Geraghty Jan 1994

Molecular And Ultrastructural Aspects Of The Adaptation Of Chlamydomonas Reinhardtii To Limiting Co2 , Anne Margaret Geraghty

Retrospective Theses and Dissertations

Change occurring in Chlamydomonas reinhardtii as cells adapt to limiting CO2 conditions were examined at both a molecular level and an ultrastructural level. It was demonstrated using antibodies that the time course of the de novo synthesis of LIP-21, a 21 kD limiting-CO2 induced polypeptide, correlates with the appearance of CO2 concentrating activity and that expression of the nuclear gene for LIP-21 is regulated at the level of translatable message. These results are consistent with LIP-21 being a component of an adaptive response to limiting CO2, such as the induction of the CO2 concentrating mechanism. Phase separation of membrane proteins ...