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Biochemistry, Biophysics, and Structural Biology Commons

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Articles 1 - 3 of 3

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Purification, Characterization And Cloning Of A Periplasmic Catalase From B Abortus And The Role It Plays In The Pathogenesis Of Brucella , Zheng Yu Sha Jan 1994

Purification, Characterization And Cloning Of A Periplasmic Catalase From B Abortus And The Role It Plays In The Pathogenesis Of Brucella , Zheng Yu Sha

Retrospective Theses and Dissertations

A periplasmic catalase has been identified and purified from Brucella abortus using a procedure which fractionates periplasmic B. abortus proteins from other cellular components while retaining biological activity. The biochemical characteristics of the purified enzyme were studied and the gene encoding the enzyme cloned and sequenced. SDS polyacrylamide gel electrophoresis of the purified enzyme revealed only a single subunit with a molecular weight of 59,000. Gel filtration indicates the native protein is a tetramer. The native protein has a visible absorption spectrum with maxima at 407, 501, and 628 nm which is typical of mammalian, but not some bacterial ...


Characterization And Immunogenicity Of The Heat Shock Protein, Hsp60 Of Brucella Abortus , Dennis Gor Jan 1994

Characterization And Immunogenicity Of The Heat Shock Protein, Hsp60 Of Brucella Abortus , Dennis Gor

Retrospective Theses and Dissertations

Brucella abortus is the causative agent of bovine brucellosis, a disease that causes spontaneous abortion in cows. The most effective form of protection against this pathogen is immunization; however, Brucella abortus strain 19 (the standard vaccine against Brucella abortus) is not completely protective. The vaccination of laboratory mice with the heat shock protein, hsp60 derived from Brucella abortus, using live attenuated recombinant Salmonella typhimurium expressing this protein (hsp60) as a delivery system, and the characterization of the immune response to Brucella abortus hsp60 over a 12 week time course following vaccination is described;Infection of BALB/c strain of laboratory ...


Molecular Mechanisms Of Mycoplasma Hyopneumoniae Adherence To Swine Respiratory Epithelial Cells, Qijing Zhang Jan 1994

Molecular Mechanisms Of Mycoplasma Hyopneumoniae Adherence To Swine Respiratory Epithelial Cells, Qijing Zhang

Retrospective Theses and Dissertations

A microtiter plate adherence assay for Mycoplasma hyopneumoniae was established by using purified swine tracheal cilia which are the natural targets for the mycoplasma. M. hyopneumoniae bound specifically to solubilized cilia immobilized onto microtiter plates. Dextran sulfate, heparin, chondroitin sulfate, laminin, mucin, and fucoidan significantly inhibited binding of the mycoplasmas to cilia. Heparin, mucin, fucoidan, and chondroitin sulfate interacted with the adhesins on the surface of mycoplasmas, whereas laminin blocked the receptors in cilia. Treatment of cilia with neuraminidase appeared to promote adherence of the mycoplasmas; whereas, treatment of cilia with sodium metaperiodate decreased the binding. In the second study ...