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Articles 1 - 29 of 29

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Tailoring The Ph Dependence Of Glucoamylase From Aspergillus Awamori By Mutagenesis , Ufuk Bakir Jan 1993

Tailoring The Ph Dependence Of Glucoamylase From Aspergillus Awamori By Mutagenesis , Ufuk Bakir

Retrospective Theses and Dissertations

A program of cassette mutagenesis was undertaken in the active site of Aspergillus awamori glucoamylase, mainly to alter the effect of pH dependence of the enzyme but also to determine possible functions of the mutated residues. For this purpose, nine single amino acid mutants were prepared and expressed in Saccharomyces cerevisiae, and their effects were determined by using steady-state enzyme kinetics;Three different groups of mutations were designed. In the first group, Glu179 → Asp, Asp176 → Glu, and Glu180 → Asp mutations were prepared. The Glu179 → Asp mutation led to a very large decrease in k[subscript] cat but no change in ...


Identification And Characterization Of Mycoplasma Promoters , Kevin Lee Knudtson Jan 1993

Identification And Characterization Of Mycoplasma Promoters , Kevin Lee Knudtson

Retrospective Theses and Dissertations

The development of a reporter gene system for mycoplasmas was sought to be able to examine gene regulation in mycoplasmas. Initial studies were designed to examine whether the Escherichia coli lacZ gene could be used as a reporter gene in mycoplasmas. The test system examined the ability of the Mycoplasma capricolum rrnA P2 or E. coli rrnB P1 promoters to initiate the transcription of trp[superscript]'-lacZYA in Acholeplasma ISM1499. The results showed that the M. capricolum, but not the E. coli promoter, could promote the expression of lacZ. Because the test system was based on a translational fusion that ...


Characterization Of The 31-Kda Antigen Gene Of Haemophilus Somnus And Its Product , Jonghwa Won Jan 1993

Characterization Of The 31-Kda Antigen Gene Of Haemophilus Somnus And Its Product , Jonghwa Won

Retrospective Theses and Dissertations

Consistent immunoreactivities of the 31-, 40- and 78-kDa proteins of Haemophilus somnus were observed in immunoblots with bovine antisera to H. somnus. A genomic library of H. somnus 8025 DNA was constructed in plasmid pUC19 and 45 recombinants expressed immunoreactive proteins in Western blots using bovine antiserum. Ten of the recombinants expressing a 31-kDa protein caused lysis of bovine erythrocytes. Restriction endonuclease mapping indicated that the hemolytic recombinants shared an approximately 1.7-kb BglII fragment. Southern blot analysis using the BglII fragment as a probe revealed homology among the recombinants and the presence of an identically sized BglII fragment in ...


Refinement Of The Modified Relative Dose Response (Mrdr) Assay As A Method To Assess Vitamin A Status Of Humans , Sherry Ann Tanumihardjo Jan 1993

Refinement Of The Modified Relative Dose Response (Mrdr) Assay As A Method To Assess Vitamin A Status Of Humans , Sherry Ann Tanumihardjo

Retrospective Theses and Dissertations

Vitamin A deficiency continues to be a public health problem in developing countries. Although overt clinical signs of deficiency are disappearing as industrialization progresses, a marginal vitamin A status is still prevalent;Because plasma retinol concentrations are homeostatically controlled throughout a wide range of liver reserves of vitamin A, serum retinol concentrations alone are not a good indicator of vitamin A status. Several methods have been developed, including the modified relative dose response (MRDR) assay, which diagnose the vitamin A status of both communities and individuals. The current work refines the use of the MRDR in assessing the vitamin A ...


Molecular Analysis Of Protein Kinase C In The Sea Urchin Lytechinus Pictus , Terese Louise Rakow Jan 1993

Molecular Analysis Of Protein Kinase C In The Sea Urchin Lytechinus Pictus , Terese Louise Rakow

Retrospective Theses and Dissertations

Protein kinase C (PKC) was originally described as a serine threonine kinase in brain tissue. This activity was found to be dependent upon Ca[superscript]2+ and phosphatidyl serine. Today protein kinase C consists of a family of kinases with nine known members. Some members are Ca[superscript]2+-independent isozymes, and all of them have subtle activation differences. The multiple isozymes with varying activities in addition to different tissue distributions lead to the idea that members of the family have different functions. PKC has been well studied in mammalian systems and it is concluded that PKC is activated in ...


Characterization Of Cis-Acting Replication Control Elements In Ribosomal Rna Genes Of Tetrahymena Thermophila , Wen-Ling Shaiu Jan 1993

Characterization Of Cis-Acting Replication Control Elements In Ribosomal Rna Genes Of Tetrahymena Thermophila , Wen-Ling Shaiu

Retrospective Theses and Dissertations

This dissertation describes the identification and characterization of specific cis-acting replication control elements in the replication origin of Tetrahymena rDNA. Earlier studies of a mutant rDNA allele, C3-rmm1, identified a cis-acting sequence (the Type I repeat) within the 5[superscript]' non-transcribed spacer of rDNA that affects rDNA replication (Larson et al. 1986). Characterization of two additional, independently-isolated mutants (C3-rmm3 and C3-rmm4) and a revertant of C3-rmm4 strongly argue that the Type I repeat is indeed a cis-acting replication control element. Mutations causing rDNA replication defects were identified in copies of the Type I repeat that are separated by >600 bp ...


Isolation And Identification Of Root-Inhibiting Compounds From Corn Gluten Meal , Dianna Lan-Ying Liu Jan 1993

Isolation And Identification Of Root-Inhibiting Compounds From Corn Gluten Meal , Dianna Lan-Ying Liu

Retrospective Theses and Dissertations

Public awareness and concern for environmental protection and human safety has led to the search for naturally occurring compounds that are able to inhibit growth and development of weed plants. It has been reported that corn gluten meal is useful as a natural preemergence herbicide and fertilizer material for various plant production systems. The active components responsible for the herbicidal activity of corn gluten meal could potentially be used as natural herbicides;Based on the results from greenhouse and growth chamber bioassays using three species of grass plants, it was found that samples of hydrolyzed protein from corn gluten meal ...


Enhancement Of Polyelectrolyte Precipitation Through The Genetic Fusion Of Charged Polypeptides To Enzymes , Mark Quinn Niederauer Jan 1993

Enhancement Of Polyelectrolyte Precipitation Through The Genetic Fusion Of Charged Polypeptides To Enzymes , Mark Quinn Niederauer

Retrospective Theses and Dissertations

The focus of this work was to investigate the enhancement of polyelectrolyte precipitation through the genetic alteration of the target protein. The dissertation is divided into three primary chapters: a literature review of precipitation techniques which display high selectivity, experimental work including the characterization and precipitation of the fusion enzymes, and modeling of the precipitation process;The literature review of selective precipitation techniques focuses on those techniques for which relatively high purifications can be achieved. The techniques include precipitation with polyelectrolytes, affinity ligands, metal ions, and protein-binding dyes. Topics discussed include the mechanisms of selectivity and precipitation, degrees of purification ...


Gene Expression Strategies Of Barley Yellow Dwarf Virus , S. P. Dinesh-Kumar Jan 1993

Gene Expression Strategies Of Barley Yellow Dwarf Virus , S. P. Dinesh-Kumar

Retrospective Theses and Dissertations

Barley yellow dwarf virus (BYDV) has a positive sense, 5.7kb RNA genome encoding at least six open reading frames (ORFs). BYDV uses variety of unusual translational strategies to express its genes. These include frameshifting in the polymerase (60K) ORF, leaky scanning at the AUGs of the overlapping coat protein (CP) and 17K ORFs, and readthrough of the CP stop codon;Full-length cDNA clone of BYDV-PAV has been constructed to understand functions of different viral genes and the roles of specific sequences in translation, replication and encapsidation. Translation in cell-free extracts using in vitro synthesized full-length transcripts resulted in expected ...


Isolation And Characterization Of Maize (Zea Mays L.) Mitochondrial Polyribosomes , Virginia Cox Crane Jan 1993

Isolation And Characterization Of Maize (Zea Mays L.) Mitochondrial Polyribosomes , Virginia Cox Crane

Retrospective Theses and Dissertations

Maize mitochondrial polyribosomes were isolated and characterized in the first and so far most complete study of the polysomal component of the translational apparatus of higher plant mitochondrial. Higher plant mitochondria are unique in that more than one, and often many, transcripts for a mitochondrial particular gene are found in abunDance; This made the characterization of the mRNA content of maize mitochondrial polyribosomes crucial to the understanding of information storage and use in these organelles;The protein gene transcript content of mitochondrial polysomes was described and compared to whole mitochondrial transcript content for seven maize mitochondrial genes. It was found ...


In Vitro Analysis Of The Self-Cleaving Satellite Rna Of Barley Yellow Dwarf Virus , Stanley Livingstone Silver Jan 1993

In Vitro Analysis Of The Self-Cleaving Satellite Rna Of Barley Yellow Dwarf Virus , Stanley Livingstone Silver

Retrospective Theses and Dissertations

The goals of this research are to begin to define key biological elements involved in the propagation of the satellite RNA associated with barley yellow dwarf virus, serotype RPV (sBYDV) and to design target-specific ribozymes against BYDV genomic RNA. sBYDV RNA is 322 nucleotides long, contains no significant open reading frames, requires BYDV for replication, encapsidation and spread and self-cleaves in the presence of Mg[superscript]2+ and neutral pH. Sequence comparison of several self-cleaving RNAs revealed the presence of a hammerhead-shaped structure spanning the cleavage site. The hammerhead is composed of 11 highly conserved, predominately single-stranded nucleotides and 3 ...


Characterization Of Dna-Binding Proteins That Recognize The Replication Origin Of The Rrna Genes Of Tetrahymena Thermophila , Angela Rae Umthun Jan 1993

Characterization Of Dna-Binding Proteins That Recognize The Replication Origin Of The Rrna Genes Of Tetrahymena Thermophila , Angela Rae Umthun

Retrospective Theses and Dissertations

Proteins that bind to specific sequences in replication origins have been shown to be important components of both prokaryotic and eukaryotic DNA replication systems. An origin of DNA replication is located within the 5[superscript]' non-transcribed spacer region of the rRNA genes (rDNA) of Tetrahymena thermophila. Previous work has shown that several cis-acting mutations responsible for rDNA replication defects lie in upstream repeats of a transcriptional promoter element, the Type I repeat;Using FE(II)EDTA cleavage footprinting and gel-mobility shift assays, I identified and characterized two distinct DNA-binding activities that interact with the Type I repeat. One of these ...


Root-Preferential Mrna Accumulation In Zea Mays , Bruce Marvin Held Jan 1993

Root-Preferential Mrna Accumulation In Zea Mays , Bruce Marvin Held

Retrospective Theses and Dissertations

To investigate the molecular basis of root development and function, I helped isolate several cDNA clones that corresponded to mRNAs that accumulated preferentially in the root of Zea mays L. (John, 1991; John et al., 1992). The purposes of the research described in this dissertation were: (1) to characterize one of these cDNA clones, pZRP4; and (2) to analyze the transcriptional regulation of root-preferential mRNA accumulation for another cDNA clone, pZRP2;Genomic Southern analysis indicated that the zrp4 gene was represented once in the corn genome. The ZRP4 mRNA accumulated preferentially in young roots, and was detected only at low ...


Characterization Of The Periplasmic Flagella Proteins Of Leptospira Spp And Cloning Of A Related Gene , Gabriel Antonio Trueba Jan 1993

Characterization Of The Periplasmic Flagella Proteins Of Leptospira Spp And Cloning Of A Related Gene , Gabriel Antonio Trueba

Retrospective Theses and Dissertations

The structure and composition of periplasmic flagella (PF) of Leptospira interrogans were analyzed. Electron microscopic observations showed that leptospiral PFs are complex structures composed of an 11.3 nm diameter core and two sheaths. Image analysis revealed sheathed PFs with two different diameters; one of 21.5 nm diameter and the other 42 nm diameter. Two dimensional protein electrophoresis of purified PFs demonstrated the presence of at least seven proteins. The molecular masses of the PF proteins ranged from 31.5 kDa to 36 kDa. Monoclonal antibodies against PF proteins and polyclonal antibodies against gel-purified PF proteins were developed. Immunoelectron ...


The Function Of Free Sulfhydryl Groups In The Catalytic Subunit Of Phosphorylase Kinase , Chiun-Jye Yuan Jan 1993

The Function Of Free Sulfhydryl Groups In The Catalytic Subunit Of Phosphorylase Kinase , Chiun-Jye Yuan

Retrospective Theses and Dissertations

Phosphorylase kinase, a serine/threonine type kinase, has been recently found to contain tyrosine kinase activity by phosphorylating angiotensin II. The tyrosine kinase activity of phosphorylase kinase requires Mn[superscript]2+. In the presence of saturable metal-nucleotide complex, MgATP, the serine kinase activity is inhibited by Mn[superscript]2+, while tyrosine kinase activity is activated. The tyrosine kinase activity in holoenzyme is Ca[superscript]2+-independent but can be regulated by autophosphorylation. One active site with two conformations is proposed; one favors seryl phosphorylation, and the second tyrosyl phosphorylation which is caused by the binding of second metal ion;The ...


Safety And Potency Testing Of Veterinary Vaccines , Louise Mary Henderson Jan 1993

Safety And Potency Testing Of Veterinary Vaccines , Louise Mary Henderson

Retrospective Theses and Dissertations

Prior to licensing of veterinary vaccines, it is essential to demonstrate that the use of the product is safe (e.g., use of the product will not have unexpected or undesirable effects on animals exposed to the agent or on the environment) and potent (e.g., produces the desired result). The projects described in this dissertation demonstrate research directed toward the development and improvement of safety and potency testing of veterinary vaccines from a regulatory perspective;In the first study, recombination between vaccine strains of pseudorabies virus (PRV) resulting in the creation of strains with undesirable virulence and antigenic combinations ...


Genetic And Physical Analysis Of The Staphylococcus Aureus Chromosome Using Two Tn917 Derivatives , Laura Lea Hruska Jan 1993

Genetic And Physical Analysis Of The Staphylococcus Aureus Chromosome Using Two Tn917 Derivatives , Laura Lea Hruska

Retrospective Theses and Dissertations

Tandem chromosomal duplications offer a means of genetic and physical mapping in microorganisms which lack defined conjugal systems. To determine if tandem chromosomal duplications occur in S. aureus, two libraries of transposon-induced auxotrophic mutations with different resistance phenotypes were needed;To complement the existing library of Tn551-induced auxotrophs, two Tn917lac derivatives were constructed: Tn917G, which contains the gentamicin-resistance gene of Tn4001, and Tn917T, which contains the tetracycline-resistance gene of pT181 and sequences of the Gram-negative cloning vector p34H. Transposon mutagenesis performed with Tn917G and Tn917T demonstrated that these two constructs are useful tools in the genetic analysis of the S ...


Genetical And Physiological Mechanisms Of Face Fly, Musca Autumnalis Degeer, Diapause , Yonggyun Kim Jan 1993

Genetical And Physiological Mechanisms Of Face Fly, Musca Autumnalis Degeer, Diapause , Yonggyun Kim

Retrospective Theses and Dissertations

Face flies, Musca autumnalis DeGeer (Diptera: Muscidae), overwinter as adults in reproductive diapause. The onset of diapause in face fly populations varies with longitude and is regulated by photoperiod. Therefore, the threshold of diapause response must be under genetic control. The mode of diapause inheritance in face flies was analyzed by crosses between high and low diapause strains. The endocrinology of diapause and reproductive developments was investigated by using in vivo and in vitro hormonal applications to see how juvenile hormone and ecdysteroid levels are regulated;Face fly diapause was polygenic with a small number of genes. Diapause inheritance did ...


Optimization Of Separation And Detection Schemes For Dna With Pulsed Field Slab Gel And Capillary Electrophoresis , David Arthur Mcgregor Jan 1993

Optimization Of Separation And Detection Schemes For Dna With Pulsed Field Slab Gel And Capillary Electrophoresis , David Arthur Mcgregor

Retrospective Theses and Dissertations

The purpose of the Human Genome Project is outlined followed by a discussion of electrophoresis in both slab gels and capillaries and its application to deoxyribonucleic acid (DNA). Techniques used to modify electroosmotic flow in capillaries are also addressed. Several separation and detection schemes for DNA via gel and capillary electrophoresis are described. Emphasis is placed on the elucidation of DNA fragment size in real time as well as shortening separation times to approximate real time monitoring;First, the migration of DNA fragment bands through a slab gel can be monitored by UV absorption at 254 nm and imaged by ...


Tissue-Specific Regulation Of 1,25-Dihydroxyvitamin D Action In The Target Tissues Of Rats , Matthew John Beckman Jan 1993

Tissue-Specific Regulation Of 1,25-Dihydroxyvitamin D Action In The Target Tissues Of Rats , Matthew John Beckman

Retrospective Theses and Dissertations

Tissue response to 1,25(OH)[subscript]2D[subscript]3 action was evaluated by examining vitamin D-dependent VDR, 24-OHase, and CaBP mRNA expressions, 24-OHase activities, and VDR concentrations in kidney and intestine. Of 4 groups of rats, 2 groups were fed an adequate vitamin D (300-600 IU/wk), 1.0-1.2% calcium (Ca) diet, designated NC, and 2 groups were fed an adequate vitamin D, 0.02% Ca diet, designated LC. One group from each diet was treated with excess vitamin D[subscript]3 (75,000 IU/wk). These groups were designated NCT and LCT, respectively;Plasma 1,25(OH ...


Site-Directed Mutagenesis To Enhance Thermostability Of Aspergillus Awamori Glucoamylase Expressed In Saccharomyces Cerevisiae , Hsiu-Mei Chen Jan 1993

Site-Directed Mutagenesis To Enhance Thermostability Of Aspergillus Awamori Glucoamylase Expressed In Saccharomyces Cerevisiae , Hsiu-Mei Chen

Retrospective Theses and Dissertations

A program of site-directed mutagenesis was undertaken to enhance thermostability of Aspergillus awamori glucoamylase. Nine mutations were designed, based on three different irreversible thermoinactivation mechanisms of glucoamylase. Each mutant was expressed in Saccharomyces cerevisiae, and their production, activities, and irreversible thermoinactivation rates were determined;First, the Asn182 and Asn395 residues were each replaced with Gln to reduce their deamidation rates. Asn395, but not Asn182, was N-glycosylated by yeast. Deletion of the N-glycan linked to Asn395 did not affect specific activity, but greatly decreased enzyme secretion and thermostability. Replacement of Asn182 by Gln did not change glucoamylase specific activity or thermostability ...


Cloning, Sequencing, And Expression Of Brucella Abortus Heat Shock 70 Gene , Emily C. L. Chin Jan 1993

Cloning, Sequencing, And Expression Of Brucella Abortus Heat Shock 70 Gene , Emily C. L. Chin

Retrospective Theses and Dissertations

Brucella abortus is a gram negative bacteria that can infect and cause serious disease in many mammals including humans. Any organism, including Brucella, that can survive as an intracellular parasite must be able to survive the stress of a foreign and often hostile environment of the host. Stress usually results in a dramatic change in gene expression including an elevated synthesis of heat shock proteins;We have subcloned, sequenced and expressed the Brucella HSP70 and the upstream sequences. The gene was put under the control of the strong "tac" promoter. However, when expressed, we saw a high accumulation of a ...


Cloning And Molecular Characterization Of Two Nuclear Genes For Zea Mays Mitochondrial Chaperonin 60 , Pamela Sue Close Jan 1993

Cloning And Molecular Characterization Of Two Nuclear Genes For Zea Mays Mitochondrial Chaperonin 60 , Pamela Sue Close

Retrospective Theses and Dissertations

Hsp60 is a mitochondrial protein first identified in Tetrahymena thermophila and Saccharomyces cerevisiae. It belongs to a class of proteins called "molecular chaperones" which prevent aggregation and precipitation of partially or completely unfolded polypeptides;Two cDNAs for maize cpn60 were identified and sequenced. Southern blots of maize DNA showed two bands hybridizing with mt-cpn60 in maize inbreds. One copy was present in Arabidopsis. Both maize genes are contained within the nuclear genome. The chromosomal location of the cpn60 genes have been mapped by RFLPs. A maize B73 genomic library has been constructed and screened for cpn60 clones and two major ...


Molecular Cloning, Expression, And Protein Interaction Of Avian Muscle Titin , Kuan Onn Tan Jan 1993

Molecular Cloning, Expression, And Protein Interaction Of Avian Muscle Titin , Kuan Onn Tan

Retrospective Theses and Dissertations

Titin (also called connectin) is a megadalton protein that constitutes up to 10% of the myofibrillar protein in skeletal and cardiac muscles. The objectives of this study were: (1) to determine a portion of the primary structure of titin; and (2) to identify interactions of cloned titin fragments with other myofibrillar proteins;A 0.2 kb cDNA clone, initially isolated by antibody screening of a chick cardiac muscle cDNA library, was used to screen a cDNA library constructed from 16-day embryonic chick cardiac muscle RNA size-selected for large RNAs. Three overlapping clones, containing 2.4 kb of titin sequence, were ...


Age, Gender And Diet Effects On Gene Expression Regulated By 1,25-Dihydroxyvitamin D3 , Julie Ann Johnson Jan 1993

Age, Gender And Diet Effects On Gene Expression Regulated By 1,25-Dihydroxyvitamin D3 , Julie Ann Johnson

Retrospective Theses and Dissertations

Age-related changes often occur in factors involved in regulation of bone mineral metabolism, including 1,25-dihydroxyvitamin D[subscript]3 (1,25(OH)[subscript]2D[subscript]3) and parathyroid hormone (PTH) in plasma, 1,25(OH)[subscript]2D[subscript]3 receptors (VDR) and vitamin D[subscript]3-dependent calcium (Ca) binding proteins (CaBP-28K and CaBP-9K) in target tissues, and the 1[alpha],25-dihydroxyvitamin D-24-hydroxylase (24-hydroxylase);Response to exogenous 1,25(OH)[subscript]2D[subscript]3 was studied in 2.5- and 18-mo-old male and female rats. Additionally, response to dietary Ca deficiency was examined in one- and 18-mo-old male rats;Plasma 1 ...


Characterization Of [Beta]-Methylcrotonyl-Coa Carboxylase Of Tomato: A Newly Identified Biotin Enzyme In Plants , Xun Wang Jan 1993

Characterization Of [Beta]-Methylcrotonyl-Coa Carboxylase Of Tomato: A Newly Identified Biotin Enzyme In Plants , Xun Wang

Retrospective Theses and Dissertations

Biotin-containing enzymes catalyze critical reactions in diverse metabolic processes. In contrast to the extensive characterizations of biotin-containing enzymes from animal and microbial sources, little is known about these enzymes from plants. I have isolated and sequenced cDNA clones coding for the biotin-containing subunit [beta]-methylcrotonyl-CoA carboxylase (MCCase) from tomato, the first molecular cloning of this enzyme from any organism, and the first molecular cloning of a plant biotin-containing enzyme. Comparison of the deduced amino acid sequence from these clones with other biotin-containing enzymes suggests that the 78 kD subunit carries biotin carboxylase and biotin carboxyl carrier functions. I have shown ...


Genetic Characterization And Molecular Cloning Of Genes Involved In Kernel Development In Zea Mays , Michael John Scanlon Jan 1993

Genetic Characterization And Molecular Cloning Of Genes Involved In Kernel Development In Zea Mays , Michael John Scanlon

Retrospective Theses and Dissertations

This dissertation describes the genetic and molecular characterization of maize genes that are required for kernel development. Maize stocks in which the Robertson's Mutator (Mu) transposable element system are especially active were used to generate 75 mutations affecting kernel development. Sixty-four kernel mutations were located to chromosome arm, and 17 were placed to more precise locations on the maize genetic map. Viability tests of kernels homozygous for defective kernel (dek) mutations revealed numerous pleiotropic effects on plants obtained from mutant kernels. Fifteen putative Mutator-induced mutations tested negative for allelism to any known gene required for kernel development, and thus ...


Acceptor Reactions And Mechanism Of Leuconostoc Mesenteroides B-512fm Dextransucrase , Aziz Tanriseven Jan 1993

Acceptor Reactions And Mechanism Of Leuconostoc Mesenteroides B-512fm Dextransucrase , Aziz Tanriseven

Retrospective Theses and Dissertations

Although Hanes-Woolf plot showed that methyl [alpha]-D-glucopyranoside was an apparent competitive inhibitor for dextransucrase, a Michaelis-Menten plot using methyl [alpha]-D-glucopyranoside in the presence of high concentration of sucrose showed that it was not a real competitive inhibitor. We have also found that methyl 6-deoxy-[alpha]-D-glucopyranoside and methyl 6-deoxy-6-fluoro-[alpha]-D-glucopyranoside were weak inhibitors, whereas the corresponding sucrose analogues 6-deoxysucrose and 6-deoxy-6-fluorosucrose are very potent inhibitors for dextransucrase. From these findings, it is concluded that the acceptor, methyl [alpha]-D-glucopyranoside, does not compete with sucrose for the sucrose binding-sites and it binds at a separate acceptor binding-site;A ...


Molecular Cloning And Characterization Of 3-Methylcrotonyl-Coa Carboxylase From Soybean , Jianping Song Jan 1993

Molecular Cloning And Characterization Of 3-Methylcrotonyl-Coa Carboxylase From Soybean , Jianping Song

Retrospective Theses and Dissertations

A cDNA clone and genes coding for the 85 kDa biotin-containing polypeptide of soybean has been isolated and sequenced. This 85 kDa polypeptide has been identified as the biotin-containing subunit of 3-methylcrotonyl-CoA carboxylase (MCCase). This is the first plant gene coding for a biotin-containing enzyme to be cloned. It is also the first time that the primary sequence of the biotin-containing subunit of MCCase has been determined;The deduced amino acid sequence of the biotin-containing subunit of MCCase of soybean shows high homology with other known biotin-containing enzymes. Amino acid sequence homology between the soybean MCCase and other biotin enzymes ...