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Articles 1 - 30 of 31

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Genetic Profiling Of Mycoplasma Hyopneumoniae , Melissa L. Madsen Jan 2005

Genetic Profiling Of Mycoplasma Hyopneumoniae , Melissa L. Madsen

Retrospective Theses and Dissertations

A microarray was constructed and applied to transcriptional profiling and genetic variation studies of Mycoplasma hyopneumoniae. The genome sequence enabled the construction of the microarray to allow a global approach to understanding fundamental processes in M. hyopneumoniae . These studies focused on whether M. hyopneumoniae regulates its genes under different environmental conditions and if genetic changes can be correlated with virulence. The microarray consisted of 632 open reading frames represented by polymerase chain reaction products and were used in a two-color experimental design. Data were analyzed using a mixed linear statistical model. Unique features implemented in these studies included the printing ...


The Role Of Rev-Sr Protein Interactions In The Regulation Of Equine Infectious Anemia Virus Replication , Gregory Saang Park Jan 2003

The Role Of Rev-Sr Protein Interactions In The Regulation Of Equine Infectious Anemia Virus Replication , Gregory Saang Park

Retrospective Theses and Dissertations

Equine infectious anemia virus (EIAV) is a member of the lentivirus subfamily of retroviruses that produces a variable clinical disease course characterized as acute, chronic, and inapparent. The clinical signs can vary according to the stage of disease, and generally correlate with levels of virus replication. As with other retroviruses, EIAV utilizes both RNA and proteins to produce alternatively spliced transcripts required for virus replication. EIAV encodes a protein called Rev, which functions by binding unspliced and singly spliced viral mRNAs in the nucleus at a sequence called the Rev responsive element (RRE) and exporting them into the cytoplasm. Rev ...


Genetic And Phenotypic Variation Of The Equine Infectious Anemia Virus Surface Unit Envelope Glycoprotein During Disease Progression , Brett Alan Sponseller Jan 2003

Genetic And Phenotypic Variation Of The Equine Infectious Anemia Virus Surface Unit Envelope Glycoprotein During Disease Progression , Brett Alan Sponseller

Retrospective Theses and Dissertations

Lentiviruses are single-stranded RNA viruses generally associated with chronic diseases of the immune and central nervous systems. In contrast to the insidious, progressive nature of most lentiviral diseases, equine infectious anemia virus (EIAV) infection results in rapid onset of a variable disease course in equids. Acute disease is accompanied with high-titered viremia, thrombocytopenia, fever, depression, and inappetance. The chronic stage is usually characterized by recurrent episodes of disease. Equids that survive recurrent disease episodes progress to the inapparent stage of disease where no clinical signs are evident; however, there is persistent, ongoing virus replication. Lentiviruses exist within the host as ...


A Candidate Gene Analysis For Response To Salmonella Enteritidis Challenge Or Vaccination In Young Chicks , Wei Liu Jan 2002

A Candidate Gene Analysis For Response To Salmonella Enteritidis Challenge Or Vaccination In Young Chicks , Wei Liu

Retrospective Theses and Dissertations

Salmonella enteritidis (SE) contamination of poultry products is a major cause of food-borne disease worldwide. The candidate gene approach for identification of quantitative trait loci (QTL) for chicken response to Salmonella enteritidis (SE) was applied in the current study. The Iowa Salmonella Response Resource Population (ISRRP), formed by crossing outbred broiler sires with three diverse, highly inbred dam lines (two major histocompatibility complex-congenic Leghorn lines G-B1 and G-B2 and one Fayoumi line) was studied. The F1 generation was evaluated as young chicks for either bacterial load in spleen and cecum after pathogenic SE inoculation, or antibody level after SE vaccination ...


Phospholipase C Of Clostridium Hemolyticum: Gene Characterization And Proof Of Its Role As A Protective Immunogen In Guinea Pigs , Paul Joseph Hauer Jan 2001

Phospholipase C Of Clostridium Hemolyticum: Gene Characterization And Proof Of Its Role As A Protective Immunogen In Guinea Pigs , Paul Joseph Hauer

Retrospective Theses and Dissertations

The phospholipase C (PLPC) gene from Clostridium hemolyticum was cloned using the polymerase chain reaction. An open reading frame which encodes a 399-amino acid protein, containing a 27-amino acid signal sequence, was identified. The molecular weight of the active protein was 42,869 daltons. A 16-amino acid N-terminal sequence determined by Edman degradation exactly matched the putative amino acid sequence of the gene product. Comparison of the nucleotide and amino acid sequences with Genebank databases demonstrated that the beta toxin of C. hemolyticum exhibits high homology with other bacterial PLPCs. The N-terminal portion of the beta toxin contains the zinc-binding ...


Genetic Variation And Evolution Of Equine Infectious Anemia Virus Rev Quasispecies During Long Term Persistent Infection , Prasith Baccam Jan 2000

Genetic Variation And Evolution Of Equine Infectious Anemia Virus Rev Quasispecies During Long Term Persistent Infection , Prasith Baccam

Retrospective Theses and Dissertations

Genetic variation has been observed in many viruses. Viruses that carry their genetic information in the form of RNA exhibit high mutation rates because the viral polymerase lacks proof-reading mechanisms commonly found in DNA polymerase complexes. The combination of high mutation rates, small genome size, and high replication rates results in a population of closely related viral genotypes, which are commonly referred to as a quasispecies. A consequence of the genetic variation in viruses is possible variation in viral phenotype of the quasispecies population. Furthermore, changes in viral phenotype may be a biologically important factor in progression of disease. Here ...


Characterization Of [Alpha]-Adrenoceptor-Mediated Contractile Responses In The Isolated Bovine Tail Artery And Vein And An Investigation Of Nerve-Mediated Contraction In The Bovine Tail Artery In Response To Electrical Field Stimulation , Marina Val Ioudina Jan 2000

Characterization Of [Alpha]-Adrenoceptor-Mediated Contractile Responses In The Isolated Bovine Tail Artery And Vein And An Investigation Of Nerve-Mediated Contraction In The Bovine Tail Artery In Response To Electrical Field Stimulation , Marina Val Ioudina

Retrospective Theses and Dissertations

The purpose of this study was to identify alpha-adrenoreceptor types and subtypes mediating contractile responses to adrenergic agonists in the bovine tail artery and vein and to investigate nerve-mediated contraction in the bovine tail artery in response to electrical field stimulation. Isolated tissue bath methods were used to study isometric contractile responses in vessel rings. Electrical field stimulation, under constant current conditions, was used to study vessel contraction as a result of neurotransmitter(s) release from sympathetic nerve terminals;Evidence was obtained for the presence of functional alpha1A- and alpha1D-adrenoceptors in the tail artery. Whether functional alpha 1B-adrenoceptors are present ...


Expression Of Bovine Mammary Gland Ca2+Atpases , Siriwan Prapong Jan 2000

Expression Of Bovine Mammary Gland Ca2+Atpases , Siriwan Prapong

Retrospective Theses and Dissertations

Control of transcellular Ca2+ fluxes associated with lactation are critical to mammary function and Ca2+ homeostasis. Mammary Ca2+ transport and storage may contribute to the pathological hypocalcemia seen in dairy cows at parturition. RT-PCR and Western Blotting were used to identify which Ca2+ ATPases are present in the bovine mammary gland and in milk fat globule membranes (MFGM). PMCA1, PMCA2 and PMCA4 are plasma membrane Ca2+ ATPases expressed in the bovine mammary gland. A putative Golgi Ca2+ ATPase (bSPCA) is also found in significant amounts in the mammary gland. The expression of Ca2+ ATPases in the bovine mammary gland is ...


Characterization Of Intracellular Survival Of Mycobacterium Avium Subspecies Paratuberculosis In J774 Murine Macrophages , Jesse Michael Hostetter Jan 2000

Characterization Of Intracellular Survival Of Mycobacterium Avium Subspecies Paratuberculosis In J774 Murine Macrophages , Jesse Michael Hostetter

Retrospective Theses and Dissertations

Mycobacterium avium subspecies paratuberculosis (M. a. ptb) is the causative agent of Johne's disease, a progressive diarrheal disease of wild and domestic ruminants. Like other pathogenic species of mycobacteria, M. a. ptb is an intracellular pathogen of monocytes and monocyte-derived macrophages. In this dissertation we tested the hypothesis that M. a. ptb survives within macrophages by inhibition of acidification and maturation of the phagosomal compartment in which it resides;Chapter 1 consists of a general introduction and a literature review of recent reports concerning normal phagosomal maturation and maturation of the mycobacterial phagosome. Chapter 2 characterizes acidification of the ...


Genetic And Molecular Characterization Of The Rev Protein Of Equine Infectious Anemia Virus , Michael Andrew Belshan Jan 1999

Genetic And Molecular Characterization Of The Rev Protein Of Equine Infectious Anemia Virus , Michael Andrew Belshan

Retrospective Theses and Dissertations

Members of the lentivirus subfamily of retroviruses are characterized as causing slow, chronic disease. Atypical of other lentiviruses, such as human immunodeficiency virus type 1, equine infectious anemia virus (EIAV) may produce a rapid, variable disease course in horses. Infected horses may undergo an, acute episode of disease involving viremia, fever, and thrombocytopenia. Following this acute period, horses may resolve to an inapparent infection or suffer a chronic period of additional cycles of viremia, fever, and thrombocytopenia. Horses which survive clinical episodes usually become inapparent carriers of the virus for life. Numerous virus and host factors contribute to the phenotypic ...


The Effects Of Neutrophil-Mediated Damage In Acute Mannheimia (Pasteurella) Haemolytica Pneumonia , Zaher Ahmad Khalil Radi Jan 1999

The Effects Of Neutrophil-Mediated Damage In Acute Mannheimia (Pasteurella) Haemolytica Pneumonia , Zaher Ahmad Khalil Radi

Retrospective Theses and Dissertations

ICAM-1 expression in cattle with Bovine Leukocyte Adhesion Deficiency (BLAD) vs. cattle without BLAD in Mannheimia haemolytica (MH) pneumonia at 2 or 4 hours PI was assessed using in situ hybridization. There was a significant increase in ICAM-1 in bronchiolar and alveolar epithelium and endothelium of arteries and veins in both BLAD and non-BLAD MH-inoculated cattle compared to controls. Highest intensity of ICAM-1 was present in bronchioles while bronchi had the lowest. Most alveolar macrophages and neutrophils in infected lungs expressed ICAM-1. ICAM-1 expression was generally increased in infected BLAD cattle at 2 hours PI compared to non-BLAD but not ...


Sequence And Functional Analysis Of The P97 Swine Cilium Adhesin Gene Of Mycoplasma Hyopneumoniae , Tsungda Hsu Jan 1997

Sequence And Functional Analysis Of The P97 Swine Cilium Adhesin Gene Of Mycoplasma Hyopneumoniae , Tsungda Hsu

Retrospective Theses and Dissertations

Mycoplasma hyopneumoniae causes swine enzootic pneumonia, an important disease in the swine industry. Adherence of M. hyopneumoniae to the cilia of the tracheal epithelial cells is required to establish infection. Previous studies have identified a 97-kDa protein (P97) as the putative ciliary adhesin. To further characterize the P97 protein, the gene has been cloned, its DNA sequence analyzed, and the function of the P97 protein expressed in Escherichia coli studied. These results demonstrated that recombinant P97 has swine ciliary adherence activity. Further analysis revealed that the P97 gene was translated as a 124.9-kilodalton (kDa) protein and subjected to proteolytic ...


Cloning, Expression And Bioactivity Of Porcine Soluble Tnf Receptor 1 And The Comparison Of Pk(15) And Wehi 164(13)-Based Tnf Bioassays , Nancy A. Maroushek Boury Jan 1997

Cloning, Expression And Bioactivity Of Porcine Soluble Tnf Receptor 1 And The Comparison Of Pk(15) And Wehi 164(13)-Based Tnf Bioassays , Nancy A. Maroushek Boury

Retrospective Theses and Dissertations

Tumor necrosis factor (TNF) is a pro-inflammatory cytokine that activates both leukocytes and endothelium and facilitates the movement of inflammatory cells from circulation to sites of infection or injury. This activation may be beneficial and aid in host defense, or may be detrimental and mediate part of the pathophysiology of disease. Several porcine diseases, such as salmonellosis and mycoplasmal pneumonia, cause an increase in TNF production, which may be either beneficial or harmful to swine health. Two related studies were completed to develop assays and reagents to further study the role of TNF in porcine disease;In the first study ...


Cloning Of Bovine Intercellular Adhesion Molecules And Characterization Of Expression Of Neutrophil Adhesion Molecules In Periparturient Cows And Calves , Eunkyung Lee Jan 1996

Cloning Of Bovine Intercellular Adhesion Molecules And Characterization Of Expression Of Neutrophil Adhesion Molecules In Periparturient Cows And Calves , Eunkyung Lee

Retrospective Theses and Dissertations

The interactions of circulating leukocytes with the endothelium represent a key point in the effector functions of the immune system by regulating the specificity and strength of cell-cell interactions. The recruitment of neutrophils to sites of inflammation is initiated by chemoattractants. The initial contact of neutrophils on vascular endothelial cells is a rolling interaction mediated by members of the selectin family. These include E-selectin and P-selectin on the surface of activated endothelial cells, and L-selectin on neutrophils. The subsequent activation of leukocyte [beta]2 integrins, which bind to the glycoprotein intercellular adhesion molecule-1 (ICAM-1) on endothelial cells, is essential for ...


Characterization Of Membrane-Associated Nuclease Activity In Mycoplasma Pulmonis , Karalee Jean Jarvill-Taylor Jan 1996

Characterization Of Membrane-Associated Nuclease Activity In Mycoplasma Pulmonis , Karalee Jean Jarvill-Taylor

Retrospective Theses and Dissertations

Mycoplasmas are deficient in numerous biosynthetic pathways. Consequently, growth is dependent upon the acquisition of structural and macromolecular precursors from the environment. These precursors include phospholipids, fatty acids and nucleotide bases. DNases and RNases are postulated to be involved in the acquisition of nucleotides. The goals of this study were to determine if nuclease activities are universally associated with mycoplasmas, characterize the nucleases by subcellular distribution and apparent molecular weight, and to identify and analyze the nuclease gene(s) in Mycoplasma pulmonis;Nuclease activity was present in all mycoplasma species tested. The level of activity did not correlate with virulence ...


Genetic And Biochemical Characterization Of Brucella Abortus Strain Rb51 , Allen Elroy Jensen Jan 1995

Genetic And Biochemical Characterization Of Brucella Abortus Strain Rb51 , Allen Elroy Jensen

Retrospective Theses and Dissertations

Brucella abortus strain RB51, a naturally derived rough variant, has been proposed to replace strain 19 for use as a vaccine against brucellosis in cattle. Utilizing fingerprinting and standard biotyping techniques, it was possible to differentiate RB51 from other brucellae and to ascertain the stability of RB51. Standardization of a genomic fingerprinting procedure using restriction endonuclease profiles of total DNA from Brucella are described. Although the focus was principally on B. abortus isolates from cattle, the investigation included field isolates from bison and elk, reference strains, vaccinal strains, and genetically-engineered brucellae;Pulsed-field gel electrophoresis profiles of the reference type strains ...


Characterization Of A Rat Clonal [Beta]-Cell Line, Rinm5f, In A Passage- And Morphology-Dependent Manner , Bum-Sup Lee Jan 1995

Characterization Of A Rat Clonal [Beta]-Cell Line, Rinm5f, In A Passage- And Morphology-Dependent Manner , Bum-Sup Lee

Retrospective Theses and Dissertations

A novel insulin-like immunoreactivity, adrenergic receptors (ARs) and voltage-dependent Ca[superscript]2+ channels (VDCCs) were characterized in a clonal [beta]-cell line, RINm5F, in a passage- and morphology-dependent manner. The cells exhibited two morphological features; the differentiated cells (DCs) with long neuron-like processes and the undifferentiated cells (UCs) without the processes;As morphology of the cells changed from DCs to UCs, amount of secreted insulin gradually decreased; however, insulin reacted to antiserum vs. bovine insulin (ABI) gradually increased from barely detectable amounts. Immunoblotting and immunocytochemistry showed that antiserum vs. rat insulin reacted with insulin from both cell types. ABI did ...


The Relationship Between The Calpain Enzyme System And The Postmortem Degradation Of Selected Myofibrillar Proteins , Elisabeth Jane Huff Lonergan Jan 1995

The Relationship Between The Calpain Enzyme System And The Postmortem Degradation Of Selected Myofibrillar Proteins , Elisabeth Jane Huff Lonergan

Retrospective Theses and Dissertations

The protease [mu]-calpain degrades the myofibrillar proteins titin, nebulin, filamin, desmin and troponin-T. Titin and nebulin have been reported to be affected by sample preparation. This study showed that there was little difference in the rate of degradation of the intact forms due to sample preparation (whole muscle preparations vs. purified myofibrils). The postmortem degradation of titin, nebulin, filamin, desmin, and troponin-T was monitored using SDS-PAGE and Western blotting techniques at 0, 1, 3, 7, 14, 28, and 56 days postmortem. Postmortem aged samples with lower shear force values exhibited faster degradation of the five myofibrillar proteins. Similar degradation ...


The Role(S) Of Arginine-Specific Mono(Adp-Ribosyl)Transferase In Skeletal Muscle Cells , Hui-Yu Huang Jan 1995

The Role(S) Of Arginine-Specific Mono(Adp-Ribosyl)Transferase In Skeletal Muscle Cells , Hui-Yu Huang

Retrospective Theses and Dissertations

The overall goal of this research project was to examine the biological function of arginine-specific mono(ADP-ribosyl) transferase in skeletal muscle cells. To better understand the function of this enzyme in skeletal muscle, the experimental approach to accomplish this goal consisted of two specific objectives. The first objective was to identify the protein substrates for this enzyme in myogenic cell cultures and cellular fractions. Embryonic chick primary muscle cell cultures were used as a working system for this purpose. In membrane fractions of 96-h myotube cultures incubated with [[superscript]32 P]NAD under conditions selected for mono(ADP-ribosyl)ation, proteins ...


Molecular Characterization Of Porcine Respiratory Coronavirus Isolates With Varying Pathogenicity , Eric Martin Vaughn Jan 1994

Molecular Characterization Of Porcine Respiratory Coronavirus Isolates With Varying Pathogenicity , Eric Martin Vaughn

Retrospective Theses and Dissertations

Three new isolates of porcine respiratory coronavirus (PRCV) were isolated and characterized. Two of the PRCV isolates, AR310 and LEPP, were shown to produce moderate interstitial pneumonia, whereas the third PRCV isolate, IA1894, was only mildly pathogenic in five-week-old specific pathogen free pigs. The previously described PRCV isolate ISU-1, a mildly pathogenic PRCV isolate, was also included in this study. These PRCV isolates with varying pathogenicity provided an opportunity to determine if there are certain genes that correlate with virulence, as the genes 3 and 3-1 have been hypothesized to be virulence determinants for transmissible gastroenteritis virus (TGEV) isolates. Using ...


Characterization And Immunogenicity Of The Heat Shock Protein, Hsp60 Of Brucella Abortus , Dennis Gor Jan 1994

Characterization And Immunogenicity Of The Heat Shock Protein, Hsp60 Of Brucella Abortus , Dennis Gor

Retrospective Theses and Dissertations

Brucella abortus is the causative agent of bovine brucellosis, a disease that causes spontaneous abortion in cows. The most effective form of protection against this pathogen is immunization; however, Brucella abortus strain 19 (the standard vaccine against Brucella abortus) is not completely protective. The vaccination of laboratory mice with the heat shock protein, hsp60 derived from Brucella abortus, using live attenuated recombinant Salmonella typhimurium expressing this protein (hsp60) as a delivery system, and the characterization of the immune response to Brucella abortus hsp60 over a 12 week time course following vaccination is described;Infection of BALB/c strain of laboratory ...


Molecular Mechanisms Of Mycoplasma Hyopneumoniae Adherence To Swine Respiratory Epithelial Cells, Qijing Zhang Jan 1994

Molecular Mechanisms Of Mycoplasma Hyopneumoniae Adherence To Swine Respiratory Epithelial Cells, Qijing Zhang

Retrospective Theses and Dissertations

A microtiter plate adherence assay for Mycoplasma hyopneumoniae was established by using purified swine tracheal cilia which are the natural targets for the mycoplasma. M. hyopneumoniae bound specifically to solubilized cilia immobilized onto microtiter plates. Dextran sulfate, heparin, chondroitin sulfate, laminin, mucin, and fucoidan significantly inhibited binding of the mycoplasmas to cilia. Heparin, mucin, fucoidan, and chondroitin sulfate interacted with the adhesins on the surface of mycoplasmas, whereas laminin blocked the receptors in cilia. Treatment of cilia with neuraminidase appeared to promote adherence of the mycoplasmas; whereas, treatment of cilia with sodium metaperiodate decreased the binding. In the second study ...


Purification, Characterization And Cloning Of A Periplasmic Catalase From B Abortus And The Role It Plays In The Pathogenesis Of Brucella , Zheng Yu Sha Jan 1994

Purification, Characterization And Cloning Of A Periplasmic Catalase From B Abortus And The Role It Plays In The Pathogenesis Of Brucella , Zheng Yu Sha

Retrospective Theses and Dissertations

A periplasmic catalase has been identified and purified from Brucella abortus using a procedure which fractionates periplasmic B. abortus proteins from other cellular components while retaining biological activity. The biochemical characteristics of the purified enzyme were studied and the gene encoding the enzyme cloned and sequenced. SDS polyacrylamide gel electrophoresis of the purified enzyme revealed only a single subunit with a molecular weight of 59,000. Gel filtration indicates the native protein is a tetramer. The native protein has a visible absorption spectrum with maxima at 407, 501, and 628 nm which is typical of mammalian, but not some bacterial ...


Characterization Of The 31-Kda Antigen Gene Of Haemophilus Somnus And Its Product , Jonghwa Won Jan 1993

Characterization Of The 31-Kda Antigen Gene Of Haemophilus Somnus And Its Product , Jonghwa Won

Retrospective Theses and Dissertations

Consistent immunoreactivities of the 31-, 40- and 78-kDa proteins of Haemophilus somnus were observed in immunoblots with bovine antisera to H. somnus. A genomic library of H. somnus 8025 DNA was constructed in plasmid pUC19 and 45 recombinants expressed immunoreactive proteins in Western blots using bovine antiserum. Ten of the recombinants expressing a 31-kDa protein caused lysis of bovine erythrocytes. Restriction endonuclease mapping indicated that the hemolytic recombinants shared an approximately 1.7-kb BglII fragment. Southern blot analysis using the BglII fragment as a probe revealed homology among the recombinants and the presence of an identically sized BglII fragment in ...


Safety And Potency Testing Of Veterinary Vaccines , Louise Mary Henderson Jan 1993

Safety And Potency Testing Of Veterinary Vaccines , Louise Mary Henderson

Retrospective Theses and Dissertations

Prior to licensing of veterinary vaccines, it is essential to demonstrate that the use of the product is safe (e.g., use of the product will not have unexpected or undesirable effects on animals exposed to the agent or on the environment) and potent (e.g., produces the desired result). The projects described in this dissertation demonstrate research directed toward the development and improvement of safety and potency testing of veterinary vaccines from a regulatory perspective;In the first study, recombination between vaccine strains of pseudorabies virus (PRV) resulting in the creation of strains with undesirable virulence and antigenic combinations ...


Tissue-Specific Regulation Of 1,25-Dihydroxyvitamin D Action In The Target Tissues Of Rats , Matthew John Beckman Jan 1993

Tissue-Specific Regulation Of 1,25-Dihydroxyvitamin D Action In The Target Tissues Of Rats , Matthew John Beckman

Retrospective Theses and Dissertations

Tissue response to 1,25(OH)[subscript]2D[subscript]3 action was evaluated by examining vitamin D-dependent VDR, 24-OHase, and CaBP mRNA expressions, 24-OHase activities, and VDR concentrations in kidney and intestine. Of 4 groups of rats, 2 groups were fed an adequate vitamin D (300-600 IU/wk), 1.0-1.2% calcium (Ca) diet, designated NC, and 2 groups were fed an adequate vitamin D, 0.02% Ca diet, designated LC. One group from each diet was treated with excess vitamin D[subscript]3 (75,000 IU/wk). These groups were designated NCT and LCT, respectively;Plasma 1,25(OH ...


Molecular Mechanisms Controlling Bovine Lymphocyte Homing , Brad Thomas Bosworth Jan 1991

Molecular Mechanisms Controlling Bovine Lymphocyte Homing , Brad Thomas Bosworth

Retrospective Theses and Dissertations

The cDNA clones encoding for the bovine glycoproteins CD44 and LECAM-1 (lectin cell adhesion molecule-1) were isolated and sequenced. The predicted amino acid sequences of bovine CD44 and LECAM-1 had high identities with those of human and murine CD44 and LECAM-1. The high identities of the predicted amino acid sequences of these glycoproteins in the bovine, human and mouse suggest they may have similar functions in all three species. While the overall amino acid identities were high, bovine CD44 had an area of low identity with human and murine CD44 in the extracellular domain and the cytoplasmic tail of bovine ...


Avena Phytochrome Mrna: In Vivo Localization, Degradation Products, And Half-Life , Kevin Andrew Seeley Jan 1991

Avena Phytochrome Mrna: In Vivo Localization, Degradation Products, And Half-Life , Kevin Andrew Seeley

Retrospective Theses and Dissertations

The purpose of this research is to determine the role that post-transcriptional processes play in the regulation of phytochrome (phy) steady-state messenger RNA (mRNA) levels. The control of etiolated phytochrome (phyA) and chlorophyll a/b binding protein (cab) gene transcription and steady-state mRNA levels are known to be regulated by the phyA signal transduction pathway. I performed a number of control experiments that indicate the degradation products detected in northern analysis of phyA mRNA were produced in vivo and were not an artifact of RNA isolation techniques. I have also shown that infrared (IR) irradiation has no appreciable effect on ...


Metabolism Of Cellulose By Microorganisms Of The Rumen , Warren D. Kitts Jan 1953

Metabolism Of Cellulose By Microorganisms Of The Rumen , Warren D. Kitts

Retrospective Theses and Dissertations

No abstract provided.


Dihydroxyphenylalanine Metabolism By Kidney Tissue , Robert Edward Clegg Jan 1948

Dihydroxyphenylalanine Metabolism By Kidney Tissue , Robert Edward Clegg

Retrospective Theses and Dissertations

No abstract provided.