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Articles 31 - 60 of 73

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Hole Burning With Pressure And Electric Field: A Window On The Electronic Structure And Energy Transfer Dynamics Of Bacterial Antenna Complexes , Hsing-Mei Wu Jan 1998

Hole Burning With Pressure And Electric Field: A Window On The Electronic Structure And Energy Transfer Dynamics Of Bacterial Antenna Complexes , Hsing-Mei Wu

Retrospective Theses and Dissertations

Light-harvesting complexes of cyclic symmetry from purple bacteria are studied using absorption and high pressure- and Stark-hole burning spectroscopies. The B800→B850 energy transfer rate of (~2 ps)-1 determined by hole burning and pump-probe spectroscopies is weakly dependent on B800-B850 energy gap varied by pressure and temperature. This resilience is explained in terms of a modified Forster theory with the spectral overlap provided by the B800 fluorescence origin band and weak vibronic absorption of B850. Explanations for the additional sub-picosecond relaxation channel of B800 with excitation on the blue side are given. Pressure and temperature dependent studies show that ...


Theoretical Simulations And Ultrafast Pump-Probe Spectroscopy Experiments In Pigment-Protein Photosynthetic Complexes , Daniel Richard Buck Jan 1998

Theoretical Simulations And Ultrafast Pump-Probe Spectroscopy Experiments In Pigment-Protein Photosynthetic Complexes , Daniel Richard Buck

Retrospective Theses and Dissertations

Theoretical simulations and ultrafast pump-probe laser spectroscopy experiments were used to study photosynthetic pigment-protein complexes and antennae found in green sulfur bacteria such as Prosthecochloris aestuarii, Chloroflexus aurantiacus, and Chlorobium tepidum. The work focused on understanding structure-function relationships in energy transfer processes in these complexes through experiments and trying to model that data as we tested our theoretical assumptions with calculations;Theoretical exciton calculations on tubular pigment aggregates yield electronic absorption spectra that are superimpositions of linear J-aggregate spectra. The electronic spectroscopy of BChl c/d/e antennae in light harvesting chlorosomes from Chloroflexus aurantiacus differs considerably from J-aggregate spectra ...


The Poplar-Leaf Rust Pathosystem: Inheritance Of Resistance And Pathogenic Variability , Girma Melesse Tabor Jan 1998

The Poplar-Leaf Rust Pathosystem: Inheritance Of Resistance And Pathogenic Variability , Girma Melesse Tabor

Retrospective Theses and Dissertations

Among diseases that cause serious yield reduction on Populus deltoides is leaf rust caused by Melampsora medusae. The objectives of this project were to study the pathogenic variability of M. medusae, the genetic basis of inheritance of M. medusae resistance in a family of P. deltoides; and to identify molecular markers linked to a leaf-rust resistant locus. Three pathotypes of M. medusae were identified on the basis of their differential reaction to three Populus clones. Pathotypes D-93, F-93, and IL-48 were compatible with clone 1-488 (P. X euramericana). F-93 and IL48 were also compatible with clone 57-276 (P. deltoides X ...


X-Ray Crystallographic Studies On Adenylosuccinate Synthetase From Escherichia Coli , Zhenglin Hou Jan 1998

X-Ray Crystallographic Studies On Adenylosuccinate Synthetase From Escherichia Coli , Zhenglin Hou

Retrospective Theses and Dissertations

Adenylosuccinate synthetase catalyzes the first committed step in de novo biosyntheses of AMP from IMP and aspartate, using GTP as the energy source. Metal cations, ligand-induced conformational transitions and metabolic effectors significantly influence the catalytic potential of the synthetase. The Mg2+, Mn2+, or Ca 2+ bind to the same site, coordinating alpha-, beta-, and gamma-phosphoryl groups of GTP. The level of catalysis supported by each cation is linked to its influence on the basicity of Asp13, a residue which abstracts the proton from N1 of IMP. Zn2+, a potent inhibitor of the synthetase, coordinates the beta- and gamma-phosphoryl groups of ...


On-Line Coupling Of Capillary Electrophoresis And Liquid Chromatography With Electrospray Ionization Mass Spectrometry For Protein Characterization , Liyu Yang Jan 1998

On-Line Coupling Of Capillary Electrophoresis And Liquid Chromatography With Electrospray Ionization Mass Spectrometry For Protein Characterization , Liyu Yang

Retrospective Theses and Dissertations

On-line coupling of capillary isoelectric focusing (CIEF) and liquid chromatography (LC) with electrospray ionization mass spectrometry (ESIMS) is utilized for protein characterization;A microdialysis junction, based on a microdialysis membrane connecting both a separation capillary and a short, sharply tapered microelectrospray emitter capillary, is demonstrated for CIEF-ESIMS. Besides the electrical connection across the microdialysis junction, post-run acidification via the dialysis membrane is demonstrated for enhancing the protonation and the ionization efficiency of focused proteins while maintaining separation efficiency, resolution, and sensitivity in CIEF-ESIMS;On-line combination of CIEF with ESI-FTICRMS is demonstrated for high resolution analysis of model proteins, human hemoglobin ...


An Integrated Multiple Capillary Array Electrophoresis System For High-Throughput Dna Sequencing , Xiandan Lu Jan 1998

An Integrated Multiple Capillary Array Electrophoresis System For High-Throughput Dna Sequencing , Xiandan Lu

Retrospective Theses and Dissertations

Capillary array electrophoresis system was chosen to perform DNA sequencing because of several advantages such as rapid heat dissipation, multiplexing capabilities, gel matrix filling simplicity, and the mature nature of the associated manufacturing technologies;There are two major concerns for the multiple capillary systems. One concern is inter-capillary cross-talk and the other concern is excitation and detection efficiency. Cross-talk is eliminated through proper optical coupling, good focusing and immersing capillary array into index matching fluid. A side-entry excitation scheme with orthogonal detection was established for large capillary array. Two 100 capillary array formats were used for DNA sequencing. One format ...


Monitoring Protein Refolding Using Diverse Separation And Spectroscopy Techniques , Pamela Kay Jensen Jan 1997

Monitoring Protein Refolding Using Diverse Separation And Spectroscopy Techniques , Pamela Kay Jensen

Retrospective Theses and Dissertations

Rapid growth in the biotechnology industry has led to a dramatic increase in attention to the protein folding problem. Understanding of protein folding is essential to the production of biopharmaceuticals since commercial production of recombinant proteins often requires a protein refolding process to recover high yields;Capillary zone electrophoresis (CZE) equipped with laser induced fluorescence detection (LIFD) is developed as a tool for monitoring the refolding of a model protein, phage P22 tailspike endorhamnosidase. Intermediates on the folding pathway are separated in CZE and monitored by their intrinsic tryptophan fluorescence. Monitoring refolding of tailspike by traditional methods, sodium dodecyl sulfate-polyacrylamide ...


Improved Resins And Novel Materials And Methods For Solid Phase Extraction And High Performance Liquid Chromatography , Ronald Carl Freeze Jan 1997

Improved Resins And Novel Materials And Methods For Solid Phase Extraction And High Performance Liquid Chromatography , Ronald Carl Freeze

Retrospective Theses and Dissertations

Solid-phase extraction (SPE) has grown to be one of the most widely used methods for isolation and preconcentration of a vast range of compounds from aqueous solutions. By modifying polymeric SPE resins with chelating functional groups, the selective uptake of metals was accomplished. By reducing the size of the resin beads and optimizing reaction conditions, resins were produced which had excellent capacities both in the packed-column and the suspension mode. By reducing bead sizes to 1 [mu]m, direct injection of the beads into an inductively coupled plasma (ICP) torch was possible. The resin, along with adsorbed metals, was vaporized ...


Characterization Of Biomacromolecules By Nuclear Magnetic Resonance Spectroscopy , Wayne Robert Baker Jan 1996

Characterization Of Biomacromolecules By Nuclear Magnetic Resonance Spectroscopy , Wayne Robert Baker

Retrospective Theses and Dissertations

Two proteins were examined by Nuclear Magnetic Resonance (NMR) spectroscopy. The Emb-1 protein of Daucus carota was found to have few spectral characteristics indicative of a stable tertiary structure. In particular, line-widths were narrow, chemical shift dispersion was poor and nuclear Overhauser effect spectra were sparse. The NMR spectra of Ribonuclease A was examined at a range of pH values and the chemical shifts of a selected number of resonances was plotted as a function of the pH. From these titrations curves pKa values were determined and assigned to specific ionizing groups within the protein based, in part, on physical ...


Single Cell Analysis By Capillary Electrophoresis With Laser-Induced Native Fluorescence Detection , Sheri Joanne Lillard Jan 1996

Single Cell Analysis By Capillary Electrophoresis With Laser-Induced Native Fluorescence Detection , Sheri Joanne Lillard

Retrospective Theses and Dissertations

Individual mammalian cells were analyzed by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). This technique was chosen due to its high separation efficiencies, small sample volumes and sensitive detection. Native fluorescence was used, in which the analyte was not tagged with a fluorophore. 275-nm excitation gave attomole (amol = 10-18 mol) detection limits for the intracellular species of interest. Two projects are described in which hemoglobin (Hb) variants were determined in single red blood cells. In the third experiment, individual mast cells were degranulated on-column, and exocytosis and serotonin release were monitored temporally;First, single red blood cells, in which Hb ...


Bacterial Physiology And Enzymology Of The Membrane-Associated Methane Monooxygenase And Ammonia Oxidation System From Methylococcus Capsulatus Bath, 1992-1996 , James Allan Zahn Jan 1996

Bacterial Physiology And Enzymology Of The Membrane-Associated Methane Monooxygenase And Ammonia Oxidation System From Methylococcus Capsulatus Bath, 1992-1996 , James Allan Zahn

Retrospective Theses and Dissertations

The enzymology and microbial physiology of methane and ammonia oxidation by the membrane-associated methane monooxygenase (pMMO) from the obligate methylotroph, Methylococcus capsulatus Bath were studied. Conditions leading to the stabilization of the pMMO were defined, and an active preparation of the pMMO from M. capsulatus Bath was isolated by ion exchange and hydrophobic interaction chromatography using dodecyl-[beta]-D-maltoside as the detergent. The active preparation consisted of three major polypeptides with molecular masses of 47,000 and 27,000, and 25,000 Da and contained 2.5 iron and 14.5 copper atoms per mole of enzyme. The 27,000 ...


Applications Of Partial Filling Micellar Electrokinetic Chromatography: From Electrospray Mass Spectrometry To Fluorescence Polarization Immunodetection , Wendy Marie Nelson Jan 1996

Applications Of Partial Filling Micellar Electrokinetic Chromatography: From Electrospray Mass Spectrometry To Fluorescence Polarization Immunodetection , Wendy Marie Nelson

Retrospective Theses and Dissertations

Mechanistic studies of partial filling micellar electrokinetic chromatography PF-MEKC) and a theoretical model for the prediction of separation behavior in PF-MEKC was developed in this work. Along with the theoretical development of PF-MEKC, two different detection modes were applied to PF-MEKC, in contrast to the traditional UV detection used in micellar electrokinetic chromatography (MEKC). The first detection mode coupled PF-MEKC with eletrospray ionization mass spectrometry. The second detection mode developed in this work is PF-MEKC coupled with fluorescence polarization immunodetection;First, in the mechanistic studies of PF-MEKC a theoretical model was proposed for predicting the separation behavior of triazine herbicides ...


Line Narrowing Spectroscopic Studies Of Dna-Carcinogen Adducts And Dna-Dye Complexes , Myungkoo Suh Jan 1995

Line Narrowing Spectroscopic Studies Of Dna-Carcinogen Adducts And Dna-Dye Complexes , Myungkoo Suh

Retrospective Theses and Dissertations

Laser-induced fluorescence line narrowing (FLN) and non-line narrowing (NLN) spectroscopic methods were applied to conformational studies of stable DNA adducts of the 7[beta],8[alpha]-dihydoxy-9[alpha],10[alpha]-epoxy-7,8,9, 10-tetrahydrobenzo (a) pyrene (anti-BPDE). Stereochemically distinct (+)-trans-, (-)-trans-, (+)-cis- and (-)-cis adducts of anti-BPDE bound to exocyclic amino group of the central guanine in the oligonucleotide, 5'-d(CCATCGCTACC) · (GGTAGCGATGG), exist in a mixture of conformations in frozen aqueous buffer matrices. The (+)-trans adduct adopts primarily an external conformation with a smaller fraction (~25%) exists in a partially base-stacked conformation. Both cis adducts were found to be ...


Spectral Hole Burning Studies Of Photosystem Ii , Hai-Chou Chang Jan 1995

Spectral Hole Burning Studies Of Photosystem Ii , Hai-Chou Chang

Retrospective Theses and Dissertations

Low temperature absorption and hole burning spectroscopies were applied to the D1-D2-cyt b[subscript]559, and the CP47 and CP43 antenna protein complexes of Photosystem II from higher plants. Low temperature transient and persistent hole-burning data and theoretical calculations on the kinetics and temperature dependence of the P680 hole profile are presented and provide convincing support for the linker model. Implicit in the linker model is that the 684-nm-absorbing Chl a serve to shuttle energy from the proximal antenna complex to reaction center. The stoichiometry of isolated Photosystem II Reaction Center (PSII RC) in several different preparations is also discussed ...


Effects Of Ear Developmental Temperature On Fine Structure Of Maize Starch , Ting-Jang Lu Jan 1995

Effects Of Ear Developmental Temperature On Fine Structure Of Maize Starch , Ting-Jang Lu

Retrospective Theses and Dissertations

Two genetically unrelated normal dent maize inbreds, ICI63 and ICI92, with different heterotic backgrounds were used for studying environmental temperature effects on grain yield and quality. These two maize inbreds are used in commercially available hybrids and are proprietary to ICI Seeds. The maize inbreds were grown in a greenhouse to control the growing condition of the plants. After 14 days post-pollination, the ears were wrapped in temperature control devices set at 25 or 35∘ C during the grain-filling period. Grain yield, kernel weight, and kernel density were less for ears developing at 35∘ C than for those at 25 ...


Photophysics And Activity Of Biological Systems Containing The Optical Probes 7-Azatryptophan And Its Analogs , Rebecca Lynne Rich Jan 1995

Photophysics And Activity Of Biological Systems Containing The Optical Probes 7-Azatryptophan And Its Analogs , Rebecca Lynne Rich

Retrospective Theses and Dissertations

A major use of photophysical apparatus is the study of biological systems, for example, peptides, proteins, and DNA, that contain one or more chromophores. In recent years the incorporation of nonnatural fluorescent compounds into these systems has gained great popularity. A number of optical 'tags' have been suggested as detection tools of structure and function; we propose the use of the tryptophan derivative, 7-azatryptophan, as an intrinsic probe in photophysical analyses of proteins. Not only is this chromophore spectroscopically distinct from tryptophan and all other natural amino acids, but we have demonstrated the successful incorporation of 7-azatryptophan into peptide sequences ...


Monolayer Studies Of Novel Synthetic Compounds And Biological Systems , Jingyuan Wang Jan 1994

Monolayer Studies Of Novel Synthetic Compounds And Biological Systems , Jingyuan Wang

Retrospective Theses and Dissertations

A general review on preparation and methods of characterization of monolayers was given in Chapter 1. Different projects were undertaken in the research of this dissertation. Neutron and X-ray reflection studies of fullerene C[subscript]60 and its derivative monolayers were performed in Chapter 1. Neutron and X-ray reflection measurements and surface pressure isotherms of spread films of C[subscript]60-dodecylamine adduct C[subscript]60-(NH[subscript]2(CH[subscript]2)[subscript]11CH[subscript]3][subscript]x and C[subscript]60-propylamine adduct C[subscript]60-(NH[subscript]2C[subscript]3H[subscript]7][subscript]x indicate that these C[subscript]60 ...


Cooperative Binding By A Self-Assembled Receptor: Metal In A Structural And Functional Role , Jin-Ho Lee Jan 1994

Cooperative Binding By A Self-Assembled Receptor: Metal In A Structural And Functional Role , Jin-Ho Lee

Retrospective Theses and Dissertations

Metal complexes of the amino acid, 4,4[superscript]'-phosphinylidine bis phenylalanine (PBP*, 1), self-assemble to produce a macrocyclic receptor (Co[subscript]2PBP[subscript]2, 2) with a hydrophobic cavity capable of binding aromatic compounds from water. Here we demonstrate that the metal can play a structural and a functional role: the receptor binds substrates bearing both aromatic and polar functional groups ditopically, using the hydrophobic pocket as well as the metal itself. The doubly anionic Co[superscript]2+ macrocyclic complex binds neutral aromatic guests more tightly than it does most anionic guests, as would be expected. However, carboxylates tethered ...


Photophysics Of A Novel Optical Probe-7-Azatryptophan And Its Application In The Study Of Protein-Protein Interactions , Yu Chen Jan 1994

Photophysics Of A Novel Optical Probe-7-Azatryptophan And Its Application In The Study Of Protein-Protein Interactions , Yu Chen

Retrospective Theses and Dissertations

Time-resolved and steady-state fluorescence study of the biological optical probe, 7-azatryptophan, its chromophoric moiety 7-azaindole are performed in order to explain the observation that the fluorescence spectrum of 7-azaindole (7-azatryptophan) apparently consists of one band in water ([lambda][subscript]sp\max em = 386nm) whereas in alcohols the spectrum is bimodal (eg. for methanol, [lambda][subscript]sp\max em = 374, 505nm). Careful measurements of the fluorescence decay as a function of emission wavelength indicate a small amplitude of an ~70 ps decaying component at the bluer wavelength and a rising component of the same duration at the redder wavelengths. The small ...


Sensitive Detection And Study Of Catalytic Species For Bimolecular Reactions , James William Lane Jan 1994

Sensitive Detection And Study Of Catalytic Species For Bimolecular Reactions , James William Lane

Retrospective Theses and Dissertations

In our lab, we have developed a sensitive and convenient assay for the detection of catalysts for bimolecular reactions. We have shown that catalysis of peptide synthesis by as little as 2.5 fmol (2.5 x 10[superscript]-15 mol) of a damaged enzyme can be detected using our assay. An interesting feature of our procedure is that it requires no complex apparatus, and may be carried out in parallel on many samples. We have used the assay to detect the presence of an enzyme catalyst, however the basic assay design is very versatile and may also be used ...


Optimization Of Separation And Detection Schemes For Dna With Pulsed Field Slab Gel And Capillary Electrophoresis , David Arthur Mcgregor Jan 1993

Optimization Of Separation And Detection Schemes For Dna With Pulsed Field Slab Gel And Capillary Electrophoresis , David Arthur Mcgregor

Retrospective Theses and Dissertations

The purpose of the Human Genome Project is outlined followed by a discussion of electrophoresis in both slab gels and capillaries and its application to deoxyribonucleic acid (DNA). Techniques used to modify electroosmotic flow in capillaries are also addressed. Several separation and detection schemes for DNA via gel and capillary electrophoresis are described. Emphasis is placed on the elucidation of DNA fragment size in real time as well as shortening separation times to approximate real time monitoring;First, the migration of DNA fragment bands through a slab gel can be monitored by UV absorption at 254 nm and imaged by ...


Isolation And Identification Of Root-Inhibiting Compounds From Corn Gluten Meal , Dianna Lan-Ying Liu Jan 1993

Isolation And Identification Of Root-Inhibiting Compounds From Corn Gluten Meal , Dianna Lan-Ying Liu

Retrospective Theses and Dissertations

Public awareness and concern for environmental protection and human safety has led to the search for naturally occurring compounds that are able to inhibit growth and development of weed plants. It has been reported that corn gluten meal is useful as a natural preemergence herbicide and fertilizer material for various plant production systems. The active components responsible for the herbicidal activity of corn gluten meal could potentially be used as natural herbicides;Based on the results from greenhouse and growth chamber bioassays using three species of grass plants, it was found that samples of hydrolyzed protein from corn gluten meal ...


Novel Detection Schemes And Automated Image Analysis Algorithms For Planar Chromatography And Gel Electrophoresis , Lance Bryant Koutny Jan 1992

Novel Detection Schemes And Automated Image Analysis Algorithms For Planar Chromatography And Gel Electrophoresis , Lance Bryant Koutny

Retrospective Theses and Dissertations

After a detailed introduction to Charge Coupled Devices (CCDs) and a discussion of personal computer capabilities, examples of their applications involving novel analytical techniques are presented;A new laser-based indirect fluorometric detection method for thin-layer chromatography is demonstrated with digitally controlled two-dimensional scanning and computer-aided image acquisition and vision. Utilizing this mode, universal detection is possible without derivatization, with a detection limit of 6 pg; 100 times lower than with human vision;A detection scheme for DNA in agarose slab gel electrophoresis based on ultraviolet absorption allows for convenient in situ monitoring of conventional gels via a CCD camera system ...


Fluorescence Detection For Gel And Capillary Electrophoresis , Barry L. Hogan Jan 1992

Fluorescence Detection For Gel And Capillary Electrophoresis , Barry L. Hogan

Retrospective Theses and Dissertations

Several separation and detection schemes for gel and capillary electrophoresis are described. Emphasis is placed upon direct and indirect fluorescence detection systems as utilized for the determination of proteins, peptides, and intracellular analytes;First, an indirect fluorescence detection system for the separation of proteins via gel electrophoresis is described. Quantities as low as 50 nanograms of bovine serum albumin and soybean trypsin inhibitor are separated and detected visually without the need for staining of the analytes. This is very similar to levels of protein commonly separated with gel electrophoresis;A separation and detection system utilizing indirect fluorescence detection with capillary ...


Selective Labeling Of Peptides, Metalloproteins, And Proteolytic Enzymes With Pt(Ii) Complexes And Electron-Transfer Reactions Of Native And Cross-Linked Metalloproteins , Herb M. Brothers Ii Jan 1991

Selective Labeling Of Peptides, Metalloproteins, And Proteolytic Enzymes With Pt(Ii) Complexes And Electron-Transfer Reactions Of Native And Cross-Linked Metalloproteins , Herb M. Brothers Ii

Retrospective Theses and Dissertations

The transition-metal complex (Pt(trpy)Cl) [superscript]+ selectively labels the amino acids Cys and His, but is unreactive toward Met. The (Pt(trpy)Cl) [superscript]+ complex also forms complexes with nonbiological ligands to form novel compounds with metal-metal or [pi]-[pi] interactions;The homologous gold reagent, (Au(trpy)Cl) [superscript]2+ reacts with Trp, Cys, His, and Met. The oxidative properties of the gold complex makes it an invasive reagent toward proteins, however;In reactions with proteins, the complex (Pt(trpy)Cl) [superscript]+ exhibits unexpected selectivity toward amino acid side chains in cytochromes c. His residues are labeled in greater ...


Study Of Secondary Structure Of Protein Sequences By Linear Algebra , Wei-Hua Hsieh Jan 1991

Study Of Secondary Structure Of Protein Sequences By Linear Algebra , Wei-Hua Hsieh

Retrospective Theses and Dissertations

Using encoding schemes, we study the relation between amino acids and protein structure in linear spaces;Local protein-structure prediction schemes use the amino acid sequences in a short subchain of a protein to predict the protein secondary structure of the middle residue of the chain;Two relatively reliable, objective and quantitative local prediction schemes are Robson et al.'s information theory method and and Qian & Sejnowski's neural network models. The latter achieved 64% accuracy for three-state predictions in 1989, the best prediction performance yet recorded;We assign to the 20 amino acids, through a one-to-one correspondence, the 20 columns (unit vectors) of the 20 x 20 identity matrix. Then any chain of k amino acids may be represented as a ...


Excited State Structure, Energy And Electron Transfer Dynamics Of Photosynthetic Reaction Centers: A Hole Burning Study , Deming Tang Jan 1990

Excited State Structure, Energy And Electron Transfer Dynamics Of Photosynthetic Reaction Centers: A Hole Burning Study , Deming Tang

Retrospective Theses and Dissertations

The excited state structure, early time energy and electron transfer dynamics for bacterial photosynthetic reaction centers of Rhodoseudomonas viridis, Rhodobacter sphaeroides and green plant photosynthetic reaction center of Photosystem II (PS II) have been determined by hole burning spectroscopy. Transient hole burned spectra of the bacterial reaction centers reveal a special pair Franck-Condon marker mode progression with a superimposed zero phonon hole. Such progression is found to be absent in green plant Photosystem II which raises the question of structural similarities between the PS II and bacterial reaction centers. The excited state decay times are obtained for all systems and ...


Spectral Hole Burning Studies Of Photosystem 1, J. Kevin Gillie Jan 1989

Spectral Hole Burning Studies Of Photosystem 1, J. Kevin Gillie

Retrospective Theses and Dissertations

Persistent spectral hole burning is applied to the reaction center, P700, and the light harvesting chlorophyll protein complexes of Photosystem I. A theory for solid state spectral hole burning is developed that is valid for arbitrarily strong linear electron-phonon coupling within the Condon approximation. Persistent photochemical hole burning of the reaction center P700, reveals that a broad (~300 cm[superscript] -1) hole can be burned into the adsorption profile. The hole profile and its maximum position and intensity dependence on burn wavelength are adequately fit by the electron-phonon coupling theory. The results indicate that the absorption and hole profile are ...


Mold Bran And Submerged Culture Fungal Preparations For Saccharifying Corn Fermentation Mashes , Edwin Lewis Pool Jan 1952

Mold Bran And Submerged Culture Fungal Preparations For Saccharifying Corn Fermentation Mashes , Edwin Lewis Pool

Retrospective Theses and Dissertations

No abstract provided.


Chemistry Of The [Beta]-Phenylserines, Kenneth N.F Shaw Jan 1951

Chemistry Of The [Beta]-Phenylserines, Kenneth N.F Shaw

Retrospective Theses and Dissertations

No abstract provided.