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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Probing Carbohydrate Biocatalysis Through The Use Of Electrospray Ionization Mass Spectrometry And Isothermal Titration Calorimetry , Corbin James Zea
Retrospective Theses and Dissertations
Electrospray ionization mass spectrometry,(ESI-MS)-based assays have been newly developed to study three classes of enzymes (sugar nucleotidyltransferases, glycosyltransferases, and phosphorylases) from the three distinct families of life: archaea, eukaryotes, and prokaryotes. These three classes of enzymes play vital roles in carbohydrate storage, degradation and synthesis. The sugar nucleotidyltransferases from Escherichia coli and yeast that biosynthesize UDP-glucose and ADP-glucose respectively were studied using the first ESI-MS assay. Nonlinear regression of the kinetic data was comparable to those determined from the traditional UDP-glucose dehydrogenase coupled assay. This technique allowed us to determine that the yeast sugar nucleotidyltransferase has one binding ...