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Biochemistry, Biophysics, and Structural Biology Commons

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Retrospective Theses and Dissertations

Physical Sciences and Mathematics

Analytic

Publication Year

Articles 1 - 6 of 6

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Improved Resins And Novel Materials And Methods For Solid Phase Extraction And High Performance Liquid Chromatography , Ronald Carl Freeze Jan 1997

Improved Resins And Novel Materials And Methods For Solid Phase Extraction And High Performance Liquid Chromatography , Ronald Carl Freeze

Retrospective Theses and Dissertations

Solid-phase extraction (SPE) has grown to be one of the most widely used methods for isolation and preconcentration of a vast range of compounds from aqueous solutions. By modifying polymeric SPE resins with chelating functional groups, the selective uptake of metals was accomplished. By reducing the size of the resin beads and optimizing reaction conditions, resins were produced which had excellent capacities both in the packed-column and the suspension mode. By reducing bead sizes to 1 [mu]m, direct injection of the beads into an inductively coupled plasma (ICP) torch was possible. The resin, along with adsorbed metals, was vaporized ...


Single Cell Analysis By Capillary Electrophoresis With Laser-Induced Native Fluorescence Detection , Sheri Joanne Lillard Jan 1996

Single Cell Analysis By Capillary Electrophoresis With Laser-Induced Native Fluorescence Detection , Sheri Joanne Lillard

Retrospective Theses and Dissertations

Individual mammalian cells were analyzed by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). This technique was chosen due to its high separation efficiencies, small sample volumes and sensitive detection. Native fluorescence was used, in which the analyte was not tagged with a fluorophore. 275-nm excitation gave attomole (amol = 10-18 mol) detection limits for the intracellular species of interest. Two projects are described in which hemoglobin (Hb) variants were determined in single red blood cells. In the third experiment, individual mast cells were degranulated on-column, and exocytosis and serotonin release were monitored temporally;First, single red blood cells, in which Hb ...


Applications Of Partial Filling Micellar Electrokinetic Chromatography: From Electrospray Mass Spectrometry To Fluorescence Polarization Immunodetection , Wendy Marie Nelson Jan 1996

Applications Of Partial Filling Micellar Electrokinetic Chromatography: From Electrospray Mass Spectrometry To Fluorescence Polarization Immunodetection , Wendy Marie Nelson

Retrospective Theses and Dissertations

Mechanistic studies of partial filling micellar electrokinetic chromatography PF-MEKC) and a theoretical model for the prediction of separation behavior in PF-MEKC was developed in this work. Along with the theoretical development of PF-MEKC, two different detection modes were applied to PF-MEKC, in contrast to the traditional UV detection used in micellar electrokinetic chromatography (MEKC). The first detection mode coupled PF-MEKC with eletrospray ionization mass spectrometry. The second detection mode developed in this work is PF-MEKC coupled with fluorescence polarization immunodetection;First, in the mechanistic studies of PF-MEKC a theoretical model was proposed for predicting the separation behavior of triazine herbicides ...


Optimization Of Separation And Detection Schemes For Dna With Pulsed Field Slab Gel And Capillary Electrophoresis , David Arthur Mcgregor Jan 1993

Optimization Of Separation And Detection Schemes For Dna With Pulsed Field Slab Gel And Capillary Electrophoresis , David Arthur Mcgregor

Retrospective Theses and Dissertations

The purpose of the Human Genome Project is outlined followed by a discussion of electrophoresis in both slab gels and capillaries and its application to deoxyribonucleic acid (DNA). Techniques used to modify electroosmotic flow in capillaries are also addressed. Several separation and detection schemes for DNA via gel and capillary electrophoresis are described. Emphasis is placed on the elucidation of DNA fragment size in real time as well as shortening separation times to approximate real time monitoring;First, the migration of DNA fragment bands through a slab gel can be monitored by UV absorption at 254 nm and imaged by ...


Novel Detection Schemes And Automated Image Analysis Algorithms For Planar Chromatography And Gel Electrophoresis , Lance Bryant Koutny Jan 1992

Novel Detection Schemes And Automated Image Analysis Algorithms For Planar Chromatography And Gel Electrophoresis , Lance Bryant Koutny

Retrospective Theses and Dissertations

After a detailed introduction to Charge Coupled Devices (CCDs) and a discussion of personal computer capabilities, examples of their applications involving novel analytical techniques are presented;A new laser-based indirect fluorometric detection method for thin-layer chromatography is demonstrated with digitally controlled two-dimensional scanning and computer-aided image acquisition and vision. Utilizing this mode, universal detection is possible without derivatization, with a detection limit of 6 pg; 100 times lower than with human vision;A detection scheme for DNA in agarose slab gel electrophoresis based on ultraviolet absorption allows for convenient in situ monitoring of conventional gels via a CCD camera system ...


Fluorescence Detection For Gel And Capillary Electrophoresis , Barry L. Hogan Jan 1992

Fluorescence Detection For Gel And Capillary Electrophoresis , Barry L. Hogan

Retrospective Theses and Dissertations

Several separation and detection schemes for gel and capillary electrophoresis are described. Emphasis is placed upon direct and indirect fluorescence detection systems as utilized for the determination of proteins, peptides, and intracellular analytes;First, an indirect fluorescence detection system for the separation of proteins via gel electrophoresis is described. Quantities as low as 50 nanograms of bovine serum albumin and soybean trypsin inhibitor are separated and detected visually without the need for staining of the analytes. This is very similar to levels of protein commonly separated with gel electrophoresis;A separation and detection system utilizing indirect fluorescence detection with capillary ...